Tissue Culture of the Succulent Plant Sedum clavatum

doi:10.11983/CBB15036

Abstract

Abstract: The leaf of Sedum clavatum was used to induce callus; through callus induction, bud induction, rooting and transplatation, aseptic-frequency regeneration systems were established. The optimal medium for induction was MS+3.0 mg·L^-1 6-BA+0.1 mg·L^-1NAA+1.0 mg·L^-1 KT; for redifferentiation 3/4MS+3.0 mg·L^-1 6-BA+0.3mg·L^-1 NAA; and for rooting 1/2MS+0.03 mg·L^-1 NAA. The callus induction, redifferentiation and rooting rate was 95.7%, 80% and 94.89%, respectively. Using perlite:vermiculite (1:2) as a matrix, the transplant survival rate was >90%. We have established a high-frequency regeneration system for S. clavatum.

Key words: Sedum clavatum, callus induction, plant regeneration

CLC Number:

Q945.52

Fang Liu, Yinghong Tang, Youmei Yuan, Qingquan Guo, Fan Shen, Jianrong Chen. Tissue Culture of the Succulent Plant Sedum clavatum[J]. Chinese Bulletin of Botany, 2016, 51(2): 251-256.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB15036

https://www.chinbullbotany.com/EN/Y2016/V51/I2/251

Figures/Tables 8

References 15

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Level	Factor (mg·L^-1)
Level	6-BA (A)	NAA (B)	KT (C)
1	0.5	0	0
2	1.5	0.1	0.5
3	3.0	0.2	1.0
4	4.5	0.3	1.5

Level	Factor (mg·L^-1)
Level	6-BA (A)	NAA (B)	KT (C)
1	0.5	0	0
2	1.5	0.1	0.5
3	3.0	0.2	1.0
4	4.5	0.3	1.5

No.	Concentrations of plant growth regulator (mg·L^-1)			Callus induction (%)	State of growth
No.	6-BA (A)	NAA (B)	KT (C)	Callus induction (%)	State of growth
1	0.5	0	0	20.0	Less calli could be induced; calli grew slowly and became yellow; lumps were small
2	0.5	0.1	0.5	32.5	Less calli could be induced; calli grew slowly and became yellow; lumps were small
3	0.5	0.2	1.0	47	Less calli could be induced; calli grew slowly and became light green; lumps were small
4	0.5	0.3	1.5	32.3	Less calli could be induced; calli grew slowly and became yellow; lumps were small
5	1.5	0	0.5	71.8	Less calli could be induced; calli grew quickly and became light green; lumps were small
6	1.5	0.1	0	74.9	Less calli could be induced; calli grew quickly and became light green; lumps were small
7	1.5	0.2	1.5	76.7	Less calli could be induced; calli grew quickly and became light green; lumps were small
8	1.5	0.3	1.0	74.1	Less calli could be induced; calli grew quickly and became light green; lumps were small
9	3	0	1.0	83.9	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
10	3	0.1	1.5	95.7	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
11	3	0.2	0	85.6	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
12	3	0.3	0.5	81	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
13	4.5	0	1.5	82.1	More calli could be induced; the way of callus induction is longitudinal expansion; calli grew quickly and became yellow green; lumps were larger
14	4.5	0.1	1.0	83.3	More calli could be induced; the way of callus induction is longitudinal expansion; calli grew quickly and became yellow green; lumps were larger
15	4.5	0.2	0.5	70.0	Less calli could be induced; calli could go into medium; calli grew quickly and became fuchsia; lumps were small
16	4.5	0.3	0	55.0	Less calli could be induced; calli could go into medium; calli grew quickly and became fuchsia; lumps were small

No.	Concentrations of plant growth regulator (mg·L^-1)			Callus induction (%)	State of growth
No.	6-BA (A)	NAA (B)	KT (C)	Callus induction (%)	State of growth
1	0.5	0	0	20.0	Less calli could be induced; calli grew slowly and became yellow; lumps were small
2	0.5	0.1	0.5	32.5	Less calli could be induced; calli grew slowly and became yellow; lumps were small
3	0.5	0.2	1.0	47	Less calli could be induced; calli grew slowly and became light green; lumps were small
4	0.5	0.3	1.5	32.3	Less calli could be induced; calli grew slowly and became yellow; lumps were small
5	1.5	0	0.5	71.8	Less calli could be induced; calli grew quickly and became light green; lumps were small
6	1.5	0.1	0	74.9	Less calli could be induced; calli grew quickly and became light green; lumps were small
7	1.5	0.2	1.5	76.7	Less calli could be induced; calli grew quickly and became light green; lumps were small
8	1.5	0.3	1.0	74.1	Less calli could be induced; calli grew quickly and became light green; lumps were small
9	3	0	1.0	83.9	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
10	3	0.1	1.5	95.7	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
11	3	0.2	0	85.6	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
12	3	0.3	0.5	81	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
13	4.5	0	1.5	82.1	More calli could be induced; the way of callus induction is longitudinal expansion; calli grew quickly and became yellow green; lumps were larger
14	4.5	0.1	1.0	83.3	More calli could be induced; the way of callus induction is longitudinal expansion; calli grew quickly and became yellow green; lumps were larger
15	4.5	0.2	0.5	70.0	Less calli could be induced; calli could go into medium; calli grew quickly and became fuchsia; lumps were small
16	4.5	0.3	0	55.0	Less calli could be induced; calli could go into medium; calli grew quickly and became fuchsia; lumps were small

K	6-BA (A)	NAA (B)	KT (C)
K₁	32.95	64.45	58.88
K₂	74.38	71.60	63.83
K₃	88.55	69.83	72.08
K₄	72.6	60.60	71.70
R	53.6	11.00	13.20
The optimum level	A₃	B₂	C₃
The optimum assembly	A₃B₂C₃