%A Fang Liu, Yinghong Tang, Youmei Yuan, Qingquan Guo, Fan Shen, Jianrong Chen %T Tissue Culture of the Succulent Plant Sedum clavatum %0 Journal Article %D 2016 %J Chinese Bulletin of Botany %R 10.11983/CBB15036 %P 251-256 %V 51 %N 2 %U {https://www.chinbullbotany.com/CN/abstract/article_2759.shtml} %8 2016-03-01 %X

The leaf of Sedum clavatum was used to induce callus; through callus induction, bud induction, rooting and transplatation, aseptic-frequency regeneration systems were established. The optimal medium for induction was MS+3.0 mg·L–1 6-BA+0.1 mg·L–1 NAA+1.0 mg·L–1 KT; for redifferentiation 3/4MS+3.0 mg·L–1 6-BA+0.3mg·L–1 NAA; and for rooting 1/2MS+0.03 mg·L–1 NAA. The callus induction, redifferentiation and rooting rate was 95.7%, 80% and 94.89%, respectively. Using perlite:vermiculite (1:2) as a matrix, the transplant survival rate was >90%. We have established a high-frequency regeneration system for S. clavatum.