Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (1): 140-149.DOI: 10.11983/CBB22177

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Identification of the NF-YC Genes in Hordeum vulgare and Expression Analysis Under Salt Stress

Feifei Wang, Zhenxiang Zhou, Yi Hong, Yangyang Gu, Chao Lü, Baojian Guo, Juan Zhu, Rugen Xu*()   

  1. Institutes of Agricultural Science, Yangzhou University/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou 225009, China
  • Received:2022-07-31 Accepted:2022-10-24 Online:2023-01-01 Published:2023-01-05
  • Contact: *E-mail:
  • About author:These authors contributed equally to this paper


Nuclear factor-Y (NF-Y) is a ubiquitous transcription factor in eukaryotes, which consists of three subunits, NF-YA, NF-YB and NF-YC. NF-Y is widely involved in the regulation of plant growth and development and responses to abiotic stress. In this study, we comprehensively analyzed the NF-YC gene family in barley using bioinformatics methods. First, 11 HvNF-YC members were identified based on the barley genome database, and they are distributed on 6 chromosomes of barley except the second chromosome with 0-5 introns. Phylogenetic analysis showed that the NF-YC family members of barley, Arabidopsis and rice could be divided into five groups. Gene replication analysis showed that six HvNF-YC genes had fragment duplication, and three HvNF-YC genes had tandem duplication. Analysis of cis-acting elements in promoter revealed that most HvNF-YC contained cis-acting elements related to abiotic stress and hormone responses. Analysis of the expression patterns of HvNF-YC members in different tissues at different stages showed that there were significant differences in the spatial and temporal expression of different members, among which HvNF-YC9 and 11 may play important roles in the early stage of grain development. By analyzing the changes of HvNF-YC expression in roots and leaves in salt-tolerant and salt-sensitive barley varieties, and HvNF-YC3, HvNF-YC6 and HvNF-YC10 genes were pivotal in the early stage of salt stress response; while HvNF-YC9 gene played essential roles in the late stage of long-term salt treatment. Therefore, we speculated that HvNF-YC9, 10, and 11 genes could be used as candidates to further explore the mechanism of NF-YC functions in salt tolerance, which will lay the foundation for further analysis of the function of HvNF-YC in barley salt tolerance.

Key words: NF-YC, gene family analysis, barley, salt stress, gene expression