Investigation of SlHVA22l gene regulating drought tolerance in tomato

doi:10.11983/CBB23129

Abstract

Abstract: Plants face inevitably encounter a multitude of environmental stresses during the growth and development stages. Among them, drought stress significantly hampers crop growth and reduces yield. Plant HVA22 protein characterized by the TB2/DP1 structural domain and implicate in the modulation of plant growth, development, and responses to abiotic stress. However, its precise function in the context of drought stress response in tomato remains to be elucidated. Therefore, in this study, we investigated the functional role of the tomato SlHVA22l gene in drought tolerance. The results showed that amino acid of SlHVA22l exhibits a higher degree of sequence similarity to HVA22l homologous proteins found in other dicotyledonous plants. Furthermore, the expression pattern analysis revealed a significant up-regulation of the SlHVA22l gene in response to drought stress and phytohormones (ABA and MeJA). Moreover, the drought tolerance function of the SlHVA22l gene was subsequently initially verify by yeast heterologous expression and silencing the endogenous SlHVA22l gene in tomato through VIGS. The silenced plants exhibited higher H2O2 and MDA contents, as well as lower O2- scavenging after drought treatment. Meanwhile, the activities of SOD, POD, CAT, and APX were significantly decreased in silenced plants compared to control plants. Collectively, these results indicate that the SlHVA22l gene plays an important role in tomato resistance to drought stress.

Key words: tomato, SlHVA22, drought stress, VIGS, gene expression

Laipeng Zhao, Baike Wang, Tao Yang, Ning Li, Haitao Yang, Juan Wang, Huizhuan Yan. Investigation of SlHVA22l gene regulating drought tolerance in tomato[J]. Chinese Bulletin of Botany, DOI: 10.11983/CBB23129.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB23129

References

[1]Casaretto JA, Ho T-hD(2005).Transcriptional regulation by abscisic acid in barley (Hordeum vulgare Lseeds involves autoregulation of the transcription factor HvABI5.Plant Molecular Biology, 57:21-34. [2]Chaudhary J, Khatri P, Singla P, Kumawat S, Kumari A, Vikram A, Jindal SK, Kardile H, Kumar R, Sonah H (2019)(2019).Advances in omics approaches for abiotic stress tolerance in tomato.Biology, 8:90-109. [3]Chen C-N, Chu C-C, Zentella R, Pan S-M, David Ho T-H(2002).AtHVA22 gene family in Arabidopsis: phylogenetic relationship,ABA and stress regulation,and tissue-specific expression.Plant molecular biology, 49:631-642. [4]Choudhury FK, Rivero RM, Blumwald E, Mittler R(2017).Reactive oxygen species,abiotic stress and stress combination.The Plant Journal, 90:856-867. [5]De Antoni A, Schmitzová J, Trepte H-H, Gallwitz D, Albert St(2002).Significance of GTP hydrolysis in Ypt1p-regulated endoplasmic reticulum to Golgi transport revealed by the analysis of two novel Ypt1-GAPs.Journal of Biological Chemistry, 277:41023-41031. [6]Eggert E, Obata T, Gerstenberger A, Gier K, Brandt T, Fernie AR, Schulze W, Kühn C(2016).A sucrose transporter‐interacting protein disulphide isomerase affects redox homeostasis and links sucrose partitioning with abiotic stress tolerance.Plant, cell & environment, 39:1366-1380. [7]Ferreira MDG, Castro JA, Silva RJS, Micheli F(2019).HVA22 from citrus: A small gene family whose some members are involved in plant response to abiotic stress.Plant Physiology and Biochemistry, 142:395-404. [8]Fukuda T, Saigusa T, Furukawa K, Inoue K, Yamashita S-I, Kanki T (2023)(2023).Hva22, a REEP family protein in fission yeast, promotes reticulophagy in collaboration with a receptor protein. Autophagy, .Autophagy, 19:2657-2667. [9]Grzesiak MT, Hordyńska N, Maksymowicz A, Grzesiak S, Szechyńska-Hebda M (2019).Variation among spring wheat (triticum aestivum l.) genotypes in response to the drought stress. II—Root system structure. Plants 8, 584.., :-. [10]Guo W-J, David Ho T-H(2008).An abscisic acid-induced protein,HVA22,inhibits gibberellin-mediated programmed cell death in cereal aleurone cells.Plant physiology, 147:1710-1722. [11]Hu J, Shibata Y, Voss C, Shemesh T, Li Z, Coughlin M, Kozlov MM, Rapoport TA, Prinz WA(2008).Membrane proteins of the endoplasmic reticulum induce high-curvature tubules.Science, 319:1247-1250. [12]Krishna R, Karkute SG, Ansari WA, Jaiswal DK, Verma JP, Singh M(2019).Transgenic tomatoes for abiotic stress tolerance: status and way ahead.Biotech, 9:1-14. [13]Letunic I, Bork P (2021).Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation. Nucleic acids research 49, W293-W296.., :-. [14]Li Q, Shen H, Yuan S, Dai X, Yang C (2023).miRNAs and lncRNAs in tomato: Roles in biotic and abiotic stress responses. Frontiers in Plant Science 13, 1094459.., :-. [15]Liang M, Li H, Zhou F, Li H, Liu J, Hao Y, Wang Y, Zhao H, Han S(2015).Subcellular distribution of NTL transcription factors in Arabidopsis thaliana.Traffic, 16:1062-1074. [16]Livak KJ, Schmittgen TD(2001).Analysis of relative gene expression data using real-time quantitative PCR and the 2? ΔΔCT method.methods, 25:402-408. [17]Lu P-L(2013).Physiological functional analysis of a stress-induced protein,HVA22,in Escherichia coli.Access Intternational Journals, 1:14-23. [18]Meng F, Zhao Q, Zhao X, Yang C, Liu R, Pang J, Zhao W, Wang Q, Liu M, Zhang Z (2022).A rice protein modulates endoplasmic reticulum homeostasis and coordinates with a transcription factor to initiate blast disease resistance. Cell reports 39, ., :-. [19]Rombauts S, Déhais P, Van Montagu M, Rouzé P(1999).PlantCARE,a plant cis-acting regulatory element database.Nucleic acids research, 27:295-296. [20]Sharon K, Suvarna S(2017).Cloning of HVA22 homolog from Aloe vera and preliminary study of transgenic plant development.Int. J. Pure App. Biosci, 5:1113-1121. [21]Shen Q, Chen C-N, Brands A, Pan S-M, Tuan-Hua DH(2001).The stress-and abscisic acid-induced barley gene HVA22: developmental regulation and homologues in diverse organisms.Plant molecular biology, 45:327-340. [22]Shen Q, Uknes S, Ho T(1993).Hormone response complex in a novel abscisic acid and cycloheximide-inducible barley gene.Journal of Biological Chemistry, 268:23652-23660. [23]Sun H, Li J, Li X, Lv Q, Chen L, Wang B, Li L(2022).RING E3 ubiquitin ligase TaSADR1 negatively regulates drought resistance in transgenic Arabidopsis.Plant Physiology and Biochemistry, 170:255-265. [24]Tamura K, Stecher G, Kumar S(2021).MEGA11: molecular evolutionary genetics analysis version 11.Molecular biology and evolution, 38:3022-3027. [25]Wagih O(2017).ggseqlogo: a versatile R package for drawing sequence logos.Bioinformatics, 33:3645-3647. [26]Wai AH, Waseem M, Cho L-H, Kim S-T, Lee D-j, Kim C-K, Chung M-Y (2022).Comprehensive genome-wide analysis and expression pattern profiling of the SlHVA22 gene family unravels their likely involvement in the abiotic stress adaptation of tomato. International Journal of Molecular Sciences 23, 12222.., :-. [27]Wang M, Yuan J, Qin L, Shi W, Xia G, Liu S(2020).Ta CYP 81D5,one member in a wheat cytochrome P450 gene cluster,confers salinity tolerance via reactive oxygen species scavenging.Plant biotechnology journal, 18:791-804. [28]Wang Y, Cao X, Zhang D, Li Y, Wang Q, Ma F, Xu X, Zhan X, Hu T (2023).SlGATA17, A tomato GATA protein, interacts with SlHY5 to modulate salinity tolerance and germination. Environmental and Experimental Botany 206, 105191.., :-. [29]Xiong H, Yu J, Miao J, Li J, Zhang H, Wang X, Liu P, Zhao Y, Jiang C, Yin Z(2018).Natural variation in OsLG3 increases drought tolerance in rice by inducing ROS scavenging.Plant Physiology, 178:451-467. [30]Yang Y-G, Lv W-T, Li M-J, Wang B, Sun D-M, Deng X(2013).Maize membrane-bound transcription factor Zmbzip17 is a key regulator in the cross-talk of ER quality control and ABA signaling.Plant and cell physiology, 54:2020-2033. [31]Zhang H, Yuan Y, Xing H, Xin M, Saeed M, Wu Q, Wu J, Zhuang T, Zhang X, Mao L (2023).Genome-wide identification and expression analysis of the HVA22 gene family in cotton and functional analysis of GhHVA22E1D in drought and salt tolerance. Frontiers in Plant Science 14, 1139526.., :-. [32]Zhao L, Wang B, Yang T, Yan H, Yu Q, Wang J (2023).Genome-wide identification and analysis of the evolution and expression pattern of the HVA22 gene family in three wild species of tomatoes. PeerJ 11, e14844.., :-. [33]Zhou P, An Y, Wang Z, Du H, Huang B (2014).Characterization of gene expression associated with drought avoidance and tolerance traits in a perennial grass species. PLoS One 9, e103611.., :-.