Chinese Bulletin of Botany ›› 2020, Vol. 55 ›› Issue (5): 573-576.DOI: 10.11983/CBB20074

• INVITED PROTOCOLS • Previous Articles     Next Articles

Methods for Identification and Resistance Evaluation of Barley Slow Rusting to Leaf Rust

Mingzhe Che1, Yajun Wang3, Chuangxin Ma1,2, Xiaoquan Qi1,2,*()   

  1. 1Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    2University of Chinese Academy of Sciences, Beijing 100049, China
    3Center for Desert Agriculture, Biological and Environmental Science & Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955, Saudi Arabia
  • Received:2020-05-08 Accepted:2020-07-21 Online:2020-09-01 Published:2020-09-03
  • Contact: Xiaoquan Qi

Abstract: Successful pathogen inoculation and following accurate rating are the basis of barley disease resistance studies. Here, we summarized selected barley leaf rust inoculation methods including spraying and smearing, and two evaluation indexes which are widely used in barley slow rusting evaluation. Key issues worthy affecting the assays were also discussed.

Key words: barley, leaf rust, slow rusting, inoculation, rating