Methods for Identification and Resistance Evaluation of Barley Slow Rusting to Leaf Rust

doi:10.11983/CBB20074

Chinese Bulletin of Botany ›› 2020, Vol. 55 ›› Issue (5): 573-576.DOI: 10.11983/CBB20074 cstr: 32102.14.CBB20074

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Methods for Identification and Resistance Evaluation of Barley Slow Rusting to Leaf Rust

Mingzhe Che¹, Yajun Wang³, Chuangxin Ma¹^,², Xiaoquan Qi¹^,²^,^*()

¹Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
²University of Chinese Academy of Sciences, Beijing 100049, China
³Center for Desert Agriculture, Biological and Environmental Science & Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955, Saudi Arabia

Received:2020-05-08 Accepted:2020-07-21 Online:2020-09-01 Published:2020-09-03
Contact: Xiaoquan Qi

Abstract

Abstract: Successful pathogen inoculation and following accurate rating are the basis of barley disease resistance studies. Here, we summarized selected barley leaf rust inoculation methods including spraying and smearing, and two evaluation indexes which are widely used in barley slow rusting evaluation. Key issues worthy affecting the assays were also discussed.

Key words: barley, leaf rust, slow rusting, inoculation, rating

Mingzhe Che, Yajun Wang, Chuangxin Ma, Xiaoquan Qi. Methods for Identification and Resistance Evaluation of Barley Slow Rusting to Leaf Rust[J]. Chinese Bulletin of Botany, 2020, 55(5): 573-576.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB20074

https://www.chinbullbotany.com/EN/Y2020/V55/I5/573

Figures/Tables 4

Figure 1 Representative images of barley plants just after spray inoculation and moisture treatment (A) and plants 14 days later when unediniospores became apparent (B)

Figure 2 Images displaying barley seedlings in the process of inoculation preparation (A) and the settling tower instrument for inoculation (B)

Figure 3 Statistical results of leaf rust inoculation in different barley cultivars (A) Measurements of the relative latency period (RLP50); (B) Measurements of the relative infection frequency (RIF). ***P<0.001

Figure 4 A nursery in a greenhouse (A) and infected barley leaves (B) (B) Bar=1 cm

References 8

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Methods for Identification and Resistance Evaluation of Barley Slow Rusting to Leaf Rust

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