Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (1): 108-121.DOI: 10.11983/CBB22155

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

The Regulatory Mechanism of Melatonin on Nucleic Acid Repairing of Common Bean (Phaseolus vulgaris) at the Sprout Stage Under Salt Stress

Qi Zhang1, Wenjing Zhang1, Xiankai Yuan1, Ming Li1, Qiang Zhao1, Yanli Du1, Jidao Du1,2,*()   

  1. 1College of Agriculture, Heilongjiang Bayi Agricultural Reclamation University, Daqing 163319, China
    2National Coarse Cereals Engineering Research Center, Daqing 163319, China
  • Received:2022-07-15 Accepted:2022-10-17 Online:2023-01-01 Published:2023-01-05
  • Contact: *E-mail: djdbynd@163.com

Abstract:

Common bean (Phaseolus vulgaris) is an important bean crop but it is highly susceptible to salt stress which causes yield decrease. Melatonin can improve the salt tolerance of plants. However, the mechanism of exogenous melatonin in regulating the salt tolerance of common bean has not been explored. In this study, the common bean variety Naihua common bean (GZ-YD014) was used as materials, and three treatments were set for comparisons, including water (W, control), salt stress (S), and salt stress+100 µmol∙L-1 melatonin (M+S). The results showed that salt stress inhibited the growth of sprouts, whose length, surface area, volume and diameter decreased significantly under salt stress conditions. Exogenous melatonin alleviated the inhibition of salt stress on the growth of common bean sprouts by signi-ficantly reducing the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) content, increasing protective enzymes such as peroxidase, superoxide dismutase, catalase and ascorbate peroxidase, osmotic regulators including soluble sugar and soluble protein content, auxin (IAA), gibberellin (GA), zeatin (ZT) content, and decreasing abscisic acid (ABA) content. Transcriptome analysis discovered 217 differentially expressed genes (DEGs), which were significantly enriched (P-value<0.05) in the nucleic acid-related entries by GO enrichment analysis and also in the nucleic acid damage repair (including base excision repair, mismatch repair and nucleotide excision repair) pathways by KEGG enrichment analysis (P-value<0.05). Real-time quantitative PCR and random amplified polymorphism analysis proved that the nucleic acid damage repair was a mechanism of exogenous melatonin regulating the salt tolerance of common bean. The study revealed the mechanism of exogenous melatonin regulating the salt tolerance of common bean sprouts, and provided a theoretical basis for the application of melatonin in common bean to increase yield under salt stress.

Key words: common bean, salt stress, melatonin, redox, base excision repair