Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (1): 77-89.DOI: 10.11983/CBB22138

Special Issue: 杂粮生物学专辑 (2023年58卷1期)

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Genome-wide Association Analysis of Resistance to Acanthoscelides obtectus in Common Bean

Xiaoming Li1, Lanfen Wang1, Yongsheng Tang2, Yujie Chang1, Juxiang Zhang2, Shumin Wang1, Jing Wu1,*()   

  1. 1Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    2Qujing Academy of Agricultural Sciences, Qujing 655000, China
  • Received:2022-06-30 Accepted:2022-09-19 Online:2023-01-01 Published:2023-01-05
  • Contact: *E-mail:
  • About author:These authors contributed equally to this paper

Abstract: Common beans (Phaseolus vulgaris) have rich nutritional value, and Acanthoscelides obtectus are major pests during the storage. It is a safe, economical and effective method to control the damage of A. obtectus by screening the germplasm resources of bruchid resistance. In this study, a total of 625 common bean germplasms were identified for resistance to A. obtectus by using improved indoor artificial inoculation method. Among them, two germplasms showed stable resistance in both two repeated identifications, with the percentage of damaged seeds less than 10%. Genome-wide association analysis was conducted based on 3 767 432 SNP and phenotypic data of the percentage of damaged seeds and the number of perforations from 625 common beans. Fifteen significant QTLs were associated with the percentage of damaged seeds and 8 significant QTLs were associated with the number of perforations, explaining 4.54% to 5.56% of the phenotypic variation. A total of 20 candidate genes were screened from all loci, including the genes encoding protease inhibitors, lectin, peroxidase and so on, which were related to the defense of bruchids.

Key words: common bean, Acanthoscelides obtectus, genome-wide association analysis, quantitative trait locus, resistant genes