Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (5): 606-619.DOI: 10.11983/CBB19053

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Gene Mapping and Candidate Gene Analysis of Rice Early Senescence Mutant LS-es1

Chun Zhou1,Ran Jiao1,Ping Hu2,Han Lin1,Juan Hu1,Na Xu1,Xianmei Wu2,Yuchun Rao1,*(),Yuexing Wang2,*()   

  1. 1. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China
    2. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
  • Received:2019-03-20 Accepted:2019-06-20 Online:2019-09-01 Published:2020-03-10
  • Contact: Yuchun Rao,Yuexing Wang

Abstract: Senescence is an autonomous and irreversible adaptive response at the end of plant development. The molecular mechanism related to premature senescence of leaves is important for rice genetic improvement and breeding of anti-aging varieties. LS-es1 is a stable hereditary premature early senescence mutant obtained by EMS mutagenesis of indica variety TP309. Phenotypic observation, physiological and biochemical analysis of LS-es1 and its wild type TP309 found that LS-es1 accumulated a large amount of reactive oxygen species and more cell death, while the yield-related agronomic traits of LS-es1 were significantly decreased compared to wild type TP309, which also verified the early senescence characteristics of LS-es1. Exogenous hormone treatment of LS-es1 and TP309 seedlings showed that LS-es1 was more sensitive to salicylic acid (SA), abscisic acid (ABA) and methyl jasmonate (MeJA). The LS-es1 gene was mapped to the 46.2 kb region of the long arm of rice chromosome 7 by map-based cloning, which included 8 open reading frames (ORFs). Bioinformatics analysis of the genes in this interval revealed that two candidate functional genes, Os07g0275300 and Os07g0276000, were associated with the early senescence pathway, and the expression levels of these two genes were significantly different between wild type and mutant. The results laid the foundation for further cloning of the LS-es1 gene and in-depth study of its biological function.

Key words: rice, early senescence, physiological characteristics, fine mapping, candidate genes