Progress of Cloning and Breeding Application of Blast Resistance Genes in Rice and Avirulence Genes in Blast Fungi

doi:10.11983/CBB18194

Abstract

Abstract: Rice blast, caused by Magnaporthe oryzae, is one of the most destructive diseases of rice worldwide. The identification and utilization of resistance genes are the basis and key factors for breeding resistance rice cultivars. With the availability of genomic sequences of both Oryza sativa and M. oryzae, rice has become one of the model systems for dissecting the molecular interactions between plants and pathogens. In this paper, we summarize the current status of genetics, mapping, cloning and breeding application of the genes resistant to blast. Using bioinformatics analysis, we analyzed the distribution of all NBS-LRR type of disease resistance genes on the 12 chromosomes of the rice genome and also preliminarily summarized the identified avirulence genes as well as interactions between the resistance proteins and the avirulence proteins. Finally, we analyzed and discussed the problems in rice breeding and prospects for research. This review will provide useful suggestions for further research on rice blast resistance breeding and disease resistance mechanisms.

Key words: rice blast, mapping, cloning, breeding application, avirulence genes

Dewei Yang,Mo Wang,Libo Han,Dingzhong Tang,Shengping Li. Progress of Cloning and Breeding Application of Blast Resistance Genes in Rice and Avirulence Genes in Blast Fungi[J]. Chinese Bulletin of Botany, 2019, 54(2): 265-276.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB18194

https://www.chinbullbotany.com/EN/Y2019/V54/I2/265

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References 100

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基因	染色体	供体	编码蛋白	参考文献
Pi37	1	St. No. 1	NBS-LRR	Lin et al., 2007
Pish	1	日本晴	NBS-LRR	Takahashi et al., 2010b
Pit	1	K59	NBS-LRR	Hayashi et al., 2010
Pi35	1	Hokkai 188	NBS-LRR	Fukuoka et al., 2014
Pi64	1		NBS-LRR	Ma et al., 2015
Pib	2	IR24, BL1	NBS-LRR	Wang et al., 1999
Bsrd-1	3	地谷	MYB转录因子	Li et al., 2017
pi-21	4	Owarihatamochi	富含脯氨酸蛋白	Fukuoka et al., 2009
Pi-63	4	Kahei	NBS-LRR	Xu et al., 2014
Pi2	6	Jefferson	NBS-LRR	Zhou et al., 2006
Pi9	6	75-1-127	NBS-LRR	Qu et al., 2006
Piz-t	6	Zenith	NBS-LRR	Zhou et al., 2006
Pid-2	6	地谷	类受体激酶蛋白	Chen et al., 2006
Pid-3	6	地谷	NBS-LRR	Shang et al., 2009
Pi-25	6	谷梅2号	NBS-LRR	Chen et al., 2011
Pid3-A4	6	A4 (普通野生稻)	NBS-LRR	Lv et al., 2013
Pi50	6	Er-Ba-zhan (EBZ)	NBS-LRR	Zhu et al., 2012
Pigm	6	谷梅4号	NBS-LRR	Deng et al., 2017
Pi-36	8	Q61	NBS-LRR	Liu et al., 2007
Pi-5/Pi-3/Pii	9	Tetep	NBS-LRR	Lee et al., 2009
Pii	9	Hitomebore	NBS-LRR	Takagi et al., 2013
Pi56	9	三黄占2号	NBS-LRR	Liu et al., 2013
Pikh/Pi-54	11	K3	NBS-LRR	Sharma et al., 2005
Pik-m	11	Tsuyuake	NBS-LRR	Ashikawa et al., 2008
Pb1	11	Modan	NBS-LRR	Hayashi et al., 2010
Pik	11	Kusabue	NBS-LRR	Zhai et al., 2011
Pik-p	11	K60	NBS-LRR	Yuan et al., 2011
Pia	11	Aichi Asahi	NBS-LRR	Hutin et al., 2016
Pi-1	11	LAC (根茎稻)	NBS-LRR	Hua et al., 2012
Pi54rh	11	nrcpb 002	NBS-LRR	Das et al., 2012
Pi-CO39	11	CO39 (药用野生稻)	NBS-LRR	Chauhan et al., 2002
Pi54of	11	nrcpb004	NBS-LRR	Devanna et al., 2014
PiK-h	11	K3	NBS-LRR	Zhai et al., 2014
Pike	11	籼早143	NBS-LRR	Chen et al., 2015
Piks	11	未知	NBS-LRR	GenBank: AET36547.1
Pita	12	Yashiro-mochi	NBS-LRR	Bryan et al., 2000
Ptr	12	M2353	膜蛋白	Zhao et al., 2018

基因	染色体	供体	编码蛋白	参考文献
Pi37	1	St. No. 1	NBS-LRR	Lin et al., 2007
Pish	1	日本晴	NBS-LRR	Takahashi et al., 2010b
Pit	1	K59	NBS-LRR	Hayashi et al., 2010
Pi35	1	Hokkai 188	NBS-LRR	Fukuoka et al., 2014
Pi64	1		NBS-LRR	Ma et al., 2015
Pib	2	IR24, BL1	NBS-LRR	Wang et al., 1999
Bsrd-1	3	地谷	MYB转录因子	Li et al., 2017
pi-21	4	Owarihatamochi	富含脯氨酸蛋白	Fukuoka et al., 2009
Pi-63	4	Kahei	NBS-LRR	Xu et al., 2014
Pi2	6	Jefferson	NBS-LRR	Zhou et al., 2006
Pi9	6	75-1-127	NBS-LRR	Qu et al., 2006
Piz-t	6	Zenith	NBS-LRR	Zhou et al., 2006
Pid-2	6	地谷	类受体激酶蛋白	Chen et al., 2006
Pid-3	6	地谷	NBS-LRR	Shang et al., 2009
Pi-25	6	谷梅2号	NBS-LRR	Chen et al., 2011
Pid3-A4	6	A4 (普通野生稻)	NBS-LRR	Lv et al., 2013
Pi50	6	Er-Ba-zhan (EBZ)	NBS-LRR	Zhu et al., 2012
Pigm	6	谷梅4号	NBS-LRR	Deng et al., 2017
Pi-36	8	Q61	NBS-LRR	Liu et al., 2007
Pi-5/Pi-3/Pii	9	Tetep	NBS-LRR	Lee et al., 2009
Pii	9	Hitomebore	NBS-LRR	Takagi et al., 2013
Pi56	9	三黄占2号	NBS-LRR	Liu et al., 2013
Pikh/Pi-54	11	K3	NBS-LRR	Sharma et al., 2005
Pik-m	11	Tsuyuake	NBS-LRR	Ashikawa et al., 2008
Pb1	11	Modan	NBS-LRR	Hayashi et al., 2010
Pik	11	Kusabue	NBS-LRR	Zhai et al., 2011
Pik-p	11	K60	NBS-LRR	Yuan et al., 2011
Pia	11	Aichi Asahi	NBS-LRR	Hutin et al., 2016
Pi-1	11	LAC (根茎稻)	NBS-LRR	Hua et al., 2012
Pi54rh	11	nrcpb 002	NBS-LRR	Das et al., 2012
Pi-CO39	11	CO39 (药用野生稻)	NBS-LRR	Chauhan et al., 2002
Pi54of	11	nrcpb004	NBS-LRR	Devanna et al., 2014
PiK-h	11	K3	NBS-LRR	Zhai et al., 2014
Pike	11	籼早143	NBS-LRR	Chen et al., 2015
Piks	11	未知	NBS-LRR	GenBank: AET36547.1
Pita	12	Yashiro-mochi	NBS-LRR	Bryan et al., 2000
Ptr	12	M2353	膜蛋白	Zhao et al., 2018

无毒基因	供体菌株	对应R基因	功能	编码分泌蛋白	参考文献
PWL1	WGG-FA40	未知	富含甘氨酸的带有信号肽的疏水蛋白	是	Kang et al., 1995
PWL2	Guy11	未知	富含甘氨酸的带有信号肽的疏水蛋白	是	Sweigard et al., 1995
PWL3	WGG-FA40	未知	富含甘氨酸的带有信号肽的疏水蛋白	是	Kang et al., 1995
PWL4	WGG-FA40	未知	富含甘氨酸的带有信号肽的疏水蛋白	是	Kang et al., 1995
Avr-CO39	K76-79	C039	功能未知, 仅在宿主细胞质中转录	是	Farman and Leong, 1998 Farman et al., 2002
Avr-Pita	O-137	Pi-ta	含金属蛋白酶结构域, 作用于宿主细胞质	是	Orbach et al., 2000
ACE1	Guy11	Pi-33	编码一个非核糖体多聚乙酰合酶, 该酶参与代谢的产物能够被Pi-33识别并激活免疫反应	未知	Fudal et al., 2005
Avr-Pia	Ina168	Pia	功能未知, 作用于宿主细胞质	是	Yoshida et al., 2009
Avr-Pii	Ina168	Pii	功能未知, 作用于宿主细胞质	是	Yasuda et al., 2006
Avr-Pik/km/kp	Ina168	Pik/km/kp	功能未知, 作用于宿主细胞质	是	Yoshida et al., 2009
Avr-Piz-t	81278ZB15	Piz-t	功能未知蛋白, 对基础抗性具有抑制作用	是	Li et al., 2009
Avr-Pi9	R01-1	Pi9	在水稻受到侵染时, 被转运到宿主细胞, 并且在侵染初期高表达	是	Wu et al., 2015
Avr-Pib	CHL42	Pib	编码75个残基蛋白, 包括信号肽	是	Zhang et al., 2015b
Avr-Pita1	O-137	Pita1	产生16个功能蛋白	是	Takahashi et al., 2010a
AVR-Pi54	MG-79	Pi54	功能未知, 作用于宿主细胞质	是	Ray et al., 2016
BAS1, BAS2, BAS3, BAS4	O-137	未知	它们以不同的模式分泌到寄主细胞中, 但以相容方式互作	是	Mosquera et al., 2009

无毒基因	供体菌株	对应R基因	功能	编码分泌蛋白	参考文献
PWL1	WGG-FA40	未知	富含甘氨酸的带有信号肽的疏水蛋白	是	Kang et al., 1995
PWL2	Guy11	未知	富含甘氨酸的带有信号肽的疏水蛋白	是	Sweigard et al., 1995
PWL3	WGG-FA40	未知	富含甘氨酸的带有信号肽的疏水蛋白	是	Kang et al., 1995
PWL4	WGG-FA40	未知	富含甘氨酸的带有信号肽的疏水蛋白	是	Kang et al., 1995
Avr-CO39	K76-79	C039	功能未知, 仅在宿主细胞质中转录	是	Farman and Leong, 1998 Farman et al., 2002
Avr-Pita	O-137	Pi-ta	含金属蛋白酶结构域, 作用于宿主细胞质	是	Orbach et al., 2000
ACE1	Guy11	Pi-33	编码一个非核糖体多聚乙酰合酶, 该酶参与代谢的产物能够被Pi-33识别并激活免疫反应	未知	Fudal et al., 2005
Avr-Pia	Ina168	Pia	功能未知, 作用于宿主细胞质	是	Yoshida et al., 2009
Avr-Pii	Ina168	Pii	功能未知, 作用于宿主细胞质	是	Yasuda et al., 2006
Avr-Pik/km/kp	Ina168	Pik/km/kp	功能未知, 作用于宿主细胞质	是	Yoshida et al., 2009
Avr-Piz-t	81278ZB15	Piz-t	功能未知蛋白, 对基础抗性具有抑制作用	是	Li et al., 2009
Avr-Pi9	R01-1	Pi9	在水稻受到侵染时, 被转运到宿主细胞, 并且在侵染初期高表达	是	Wu et al., 2015
Avr-Pib	CHL42	Pib	编码75个残基蛋白, 包括信号肽	是	Zhang et al., 2015b
Avr-Pita1	O-137	Pita1	产生16个功能蛋白	是	Takahashi et al., 2010a
AVR-Pi54	MG-79	Pi54	功能未知, 作用于宿主细胞质	是	Ray et al., 2016
BAS1, BAS2, BAS3, BAS4	O-137	未知	它们以不同的模式分泌到寄主细胞中, 但以相容方式互作	是	Mosquera et al., 2009