Effect of LED in Different Light Qualities on Growth of Phalaenopsis Plantlets

doi:10.11983/CBB14196

Abstract

Abstract: We used the aseptic seedlings of 2 cultivars (Phalaenopsis ssp. cv. ‘Green Bear’ and Phalaenopsis ssp. cv. ‘Big Chilli’) to investigate the effect of LED in different light qualities on multiplication and rooting in vitro. The experimental design included 15 different light sources composed of six kinds of LED light, namely RED(630), R(630)8B2, R(630)7B3, R(630)5B5, R(630)3B7, R(630)2B8, BLUE, RED(660), R(660)7B3, R(660)5B5, CW5R(630)5, Warm W, R(630)6B3FR1, R(630)4B4FR2, R(630)3B6FR1, and WFL (control). Under red light illumination, seeding growth and the number of shoots were promoted, but chlorophyll content of leaves was reduced, but it was increased under the mixture of blue and red LEDs. Blue light had a positive effect on chlorophyll content. Root activity was increased with increasing ratio of blue in the combined red and blue LEDs and increased under the three light sources supplemented with far-red light. Blue and far-red light were more effective in root length. Shoots could be more successfully induced under Warm W light. Compared with WFL light, the number of shoots of the 2 varieties was increased by 53.17% and 46.37%, respectively. Therefore, the best light recipe for the multiplication and rooting of Phalaenopsis were Warm W and R(630)3B6FR1 light. The present study provides a proof of application of LEDs in Phalaenopsis tissue culture.

Key words: light-emitting diode, light quality, Phalaenopsis, tissue culture

Guiping Ren, Xiaojing Wang, Genfa Zhu. Effect of LED in Different Light Qualities on Growth of Phalaenopsis Plantlets[J]. Chinese Bulletin of Botany, 2016, 51(1): 81-88.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB14196

https://www.chinbullbotany.com/EN/Y2016/V51/I1/81

Figures/Tables 6

References 18

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Light treatment	Factors	Wavelength
RED(630)	Red	630 nm
R(630)8B2	Red:Blue=8:2	630 nm/450-480 nm
R(630)7B3	Red:Blue=7:3	630 nm/450-480 nm
R(630)5B5	Red:Blue=5:5	630 nm/450-480 nm
R(630)3B7	Red:Blue=3:7	630 nm/450-480 nm
R(630)2B8	Red:Blue=2:8	630 nm/450-480 nm
BLUE	Blue	450-480 nm
RED(660)	Red	660 nm
R(660)7B3	Red:Blue=7:3	660 nm/450-480 nm
R(660)5B5	Red:Blue=5:5	660 nm/450-480 nm
CW5R(630)5	Cold white:Red=5:5	630 nm/400-780 nm
Warm W	Warm white LED	400 nm-780 nm (the range of 500-780 nm accounts for 91.80%)
R(630)6B3FR1	Red:Blue:Far red=6:3:1	630 nm/450-480 nm/730 nm
R(630)4B4FR2	Red:Blue:Far red=4:4:2	630 nm/450-480 nm/730 nm
R(630)3B6FR1	Red:Blue:Far red=3:6:1	630 nm/450-480 nm/730 nm
WFL	White fluorescent tube	360-750 nm

Light treatment	Factors	Wavelength
RED(630)	Red	630 nm
R(630)8B2	Red:Blue=8:2	630 nm/450-480 nm
R(630)7B3	Red:Blue=7:3	630 nm/450-480 nm
R(630)5B5	Red:Blue=5:5	630 nm/450-480 nm
R(630)3B7	Red:Blue=3:7	630 nm/450-480 nm
R(630)2B8	Red:Blue=2:8	630 nm/450-480 nm
BLUE	Blue	450-480 nm
RED(660)	Red	660 nm
R(660)7B3	Red:Blue=7:3	660 nm/450-480 nm
R(660)5B5	Red:Blue=5:5	660 nm/450-480 nm
CW5R(630)5	Cold white:Red=5:5	630 nm/400-780 nm
Warm W	Warm white LED	400 nm-780 nm (the range of 500-780 nm accounts for 91.80%)
R(630)6B3FR1	Red:Blue:Far red=6:3:1	630 nm/450-480 nm/730 nm
R(630)4B4FR2	Red:Blue:Far red=4:4:2	630 nm/450-480 nm/730 nm
R(630)3B6FR1	Red:Blue:Far red=3:6:1	630 nm/450-480 nm/730 nm
WFL	White fluorescent tube	360-750 nm

Light treatment	Green Bear			Big Chilli
Light treatment	Multiplication coefficient	Ratio (%) (number of shoot≥3)	Ratio (%) (number of shoot≥4)	Multiplication coefficient	Ratio (%) (number of shoot≥3)
RED(630)	2.85±0.16 c	51.39	25.00	2.20±0.14 g	34.55
R(630)8B2	2.26±0.13 a	38.46	11.54	1.85±0.11 cdef	18.52
R(630)7B3	2.33±0.14 a	36.84	13.16	1.78±0.12 bcdef	12.73
R(630)5B5	2.19±0.15 a	32.43	9.46	1.63±0.09 abcde	9.26
R(630)3B7	2.14±0.12 a	25.71	10.00	1.69±0.11 abcdef	14.55
R(630)2B8	2.37±0.11 a	36.90	11.90	1.50±0.08 abc	3.57
BLUE	2.27±0.13 a	33.33	14.67	1.66±0.13 abcdef	16.00
RED(660)	2.80±0.15 bc	56.25	25.00	2.00±0.12 fg	32.14
R(660)7B3	2.13±0.13 a	35.94	9.38	1.86±0.10 defg	21.57
R(660)5B5	2.39±0.14 ab	40.26	18.18	1.75±0.11 bcdef	19.23
CW5R(630)5	2.43±0.15 ab	38.67	20.00	1.94±0.12 efg	20.75
Warm W	3.14±0.17 c	62.16	36.49	2.02±0.12 fg	26.98
R(630)6B3FR1	2.32±0.17 a	34.78	14.49	1.49±0.09 ab	6.38
R(630)4B4FR2	2.29±0.19 a	31.82	13.64	1.56±0.09 abcd	8.33
R(630)3B6FR1	2.35±0.11 a	37.84	10.81	1.45±0.09 ab	5.45
WFL	2.05±0.12 a	22.08	6.49	1.38±0.07 a	1.82

Light treatment	Green Bear			Big Chilli
Light treatment	Multiplication coefficient	Ratio (%) (number of shoot≥3)	Ratio (%) (number of shoot≥4)	Multiplication coefficient	Ratio (%) (number of shoot≥3)
RED(630)	2.85±0.16 c	51.39	25.00	2.20±0.14 g	34.55
R(630)8B2	2.26±0.13 a	38.46	11.54	1.85±0.11 cdef	18.52
R(630)7B3	2.33±0.14 a	36.84	13.16	1.78±0.12 bcdef	12.73
R(630)5B5	2.19±0.15 a	32.43	9.46	1.63±0.09 abcde	9.26
R(630)3B7	2.14±0.12 a	25.71	10.00	1.69±0.11 abcdef	14.55
R(630)2B8	2.37±0.11 a	36.90	11.90	1.50±0.08 abc	3.57
BLUE	2.27±0.13 a	33.33	14.67	1.66±0.13 abcdef	16.00
RED(660)	2.80±0.15 bc	56.25	25.00	2.00±0.12 fg	32.14
R(660)7B3	2.13±0.13 a	35.94	9.38	1.86±0.10 defg	21.57
R(660)5B5	2.39±0.14 ab	40.26	18.18	1.75±0.11 bcdef	19.23
CW5R(630)5	2.43±0.15 ab	38.67	20.00	1.94±0.12 efg	20.75
Warm W	3.14±0.17 c	62.16	36.49	2.02±0.12 fg	26.98
R(630)6B3FR1	2.32±0.17 a	34.78	14.49	1.49±0.09 ab	6.38
R(630)4B4FR2	2.29±0.19 a	31.82	13.64	1.56±0.09 abcd	8.33
R(630)3B6FR1	2.35±0.11 a	37.84	10.81	1.45±0.09 ab	5.45
WFL	2.05±0.12 a	22.08	6.49	1.38±0.07 a	1.82

Light treatment	Green Bear			Big Chilli
Light treatment	FW (g)	DW (mg)	Height (mm)	FW (g)	DW (mg)	Height (mm)
RED(630)	0.94±0.05 bcde	49.56±2.81 abc	38.16±1.31 ef	0.74±0.04 bc	41.28±5.24 ab	42.77±1.11 f
R(630)8B2	0.90±0.05 bcde	49.58±2.81 abc	36.63±1.13 cde	0.81±0.05 bcd	42.01±2.45 ab	36.61±1.03 cd
R(630)7B3	0.84±0.05 abc	47.30±3.33 ab	33.15±1.00 ab	0.75±0.05 bc	42.82±2.59 abc	34.21±0.99 bc
R(630)5B5	0.79±0.05 ab	47.86±3.34 ab	34.59±0.88 bcd	0.74±0.04 bcd	41.89±2.19 ab	34.20±0.86 bc
R(630)3B7	0.71±0.05 a	43.16±2.70 a	32.94±0.77 ab	0.73±0.04 bc	46.04±2.37 bc	28.98±1.09 a
R(630)2B8	0.80±0.06 ab	45.01±3.61 a	30.94±0.78 a	0.76±0.05 bcd	43.65±2.53 bc	32.53±0.85 b
BLUE	0.79±0.05 ab	53.30±3.35 abcd	39.68±0.89 f	0.74±0.04 bc	41.88±2.46 ab	37.79±1.01 de
RED(660)	0.83±0.05 abc	47.80±2.49 ab	37.41±0.90 def	0.89±0.05 d	53.17±7.59 c	42.78±1.26 f
R(660)7B3	1.04±0.05 e	56.86±1.77 bcde	37.18±0.92 def	0.68±0.04 b	40.16±2.74 ab	32.92±1.13 b
R(660)5B5	0.95±0.05 bcde	58.26±3.12 cde	32.93±0.76 ab	0.83±0.05 cd	42.41±2.14 ab	34.37±1.14 bc
CW5R(630)5	0.99±0.05 cde	56.80±3.06 bcde	34.94±0.96 bcd	0.75±0.04 bcd	47.30±2.94 bc	38.83±1.14 de
Warm W	1.04±0.06 e	60.25±3.61 de	36.37±1.08 cde	0.81±0.05 bc	41.76±2.27 ab	40.70±1.19 ef
R(630)6B3FR1	0.88±0.05 abcde	50.44±2.82 abcd	34.96±0.98 bcd	0.70±0.04 bc	43.67±2.11 bc	32.41±0.87 b
R(630)4B4FR2	1.04±0.08 de	63.37±4.68 e	34.47±1.22 bcd	0.81±0.03 bcd	49.82±2.56 bc	33.02±1.16 b
R(630)3B6FR1	0.86±0.04 abcd	51.89±2.46 abcd	34.69±0.91 bcd	0.69±0.04 bc	42.69±1.96 abc	31.55±0.98 ab
WFL	0.87±0.06 abcde	50.63±3.17 abcd	33.74±0.83 abc	0.53±0.03 a	32.59±1.60 a	31.32±0.98 ab