植物学报 ›› 2014, Vol. 49 ›› Issue (6): 643-652.DOI: 10.3724/SP.J.1259.2014.00643 cstr: 32102.14.SP.J.1259.2014.00643
• 研究报告 • 下一篇
王翠丽1,2†, 田海霞1,2†, 汪姣姣2†, 齐天从2, 黄煌2, 任春梅1, 谢道昕2, 宋素胜2*
收稿日期:
2013-09-24
修回日期:
2014-06-02
出版日期:
2014-11-01
发布日期:
2014-11-21
通讯作者:
宋素胜
基金资助:
国家自然科学基金;国家自然科学基金;中国博士后科学基金特别资助;中国博士后科学基金特别资助
Cuili Wang1,2†, Haixia Tian1,2†, Jiaojiao Wang2†, Tiancong Qi2, Huang Huang2, Chunmei Ren1, Daoxin Xie2, Susheng Song2*
Received:
2013-09-24
Revised:
2014-06-02
Online:
2014-11-01
Published:
2014-11-21
Contact:
Susheng Song
摘要: 植物激素茉莉素作为抗性信号调控植物对腐生性病原菌和昆虫的抗性, 作为发育信号调控植物根的生长、雄蕊发育、表皮毛形成和叶片衰老。茉莉素受体COI1识别茉莉素分子, 进而与JAZ蛋白互作并诱导其降解, 继而调控多种茉莉素反应。拟南芥(Arabidopsis thaliana) IIId亚组bHLH转录因子(bHLH3、bHLH13、bHLH14和bHLH17)是JAZ的一类靶蛋白。与野生型相比, IIId亚组bHLH转录因子的单突变体对灰霉菌和甜菜夜蛾的抗性无明显差异, 而四突变体对灰霉菌和甜菜夜蛾的抗性增强。该文通过高表达bHLH17并研究其对灰霉菌和甜菜夜蛾的抗性反应, 结果显示, 被灰霉菌侵染的bHLH17高表达植株较野生型表现出更严重的病症。取食bHLH17高表达植株叶片的甜菜夜蛾幼虫体重大于取食野生型叶片的幼虫体重。bHLH17高表达抑制了茉莉素诱导的抗性相关基因(Thi2.1)和伤害响应基因(VSP2、AOS、JAZ1、JAZ9和JAZ10)的表达。原生质体转化实验显示bHLH17通过其N端行使转录抑制功能。研究结果表明, IIId亚组bHLH转录抑制因子bHLH17高表达会负调控茉莉素介导的对灰霉菌和甜菜夜蛾的抗性。
王翠丽, 田海霞, 汪姣姣, 齐天从, 黄煌, 任春梅, 谢道昕, 宋素胜. 拟南芥转录因子bHLH17负调控茉莉素介导的抗性反应. 植物学报, 2014, 49(6): 643-652.
Cuili Wang, Haixia Tian, Jiaojiao Wang, Tiancong Qi, Huang Huang, Chunmei Ren, Daoxin Xie, Susheng Song. bHLH17 Negatively Regulates Jasmonate-mediated Plant Defense Responses. Chinese Bulletin of Botany, 2014, 49(6): 643-652.
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