白菜类作物抗TuMV研究进展

doi:10.11983/CBB22061

摘要/Abstract

摘要： 白菜类作物主要包括大白菜(Brassica rapa subsp. pekinensis)、不结球白菜(B. rapa subsp. chinensis)和芜菁(B. rapa subsp. rapa)等, 可用作蔬菜、油料及饲料。白菜类作物在生产过程中常受到病毒病的危害, 其中芜菁花叶病毒(TuMV)是主要病原物。近年来, 在TuMV抗性基因的遗传分析、定位克隆及分子机制方面取得了一定研究进展, 为培育优质抗病新品种奠定了基础。该文对国内外关于白菜类作物抗TuMV的遗传分析、抗性基因与分子标记、生物信息学与组学分析以及基因工程技术在抗TuMV研究中的应用进行综述, 以期为白菜类作物抗TuMV研究和技术利用提供科学依据。

关键词: 白菜, 芜菁花叶病毒, 遗传分析, 抗性基因, 研究进展

Abstract: Brassica rapa plants, including B. rapa subsp. pekinensis, B. rapa subsp. chinensis, B. rapa subsp. rapa and so on, serve as vegetables, oil crops, and fodder. They are constantly suffering from viral diseases, among which turnip mosaic virus (TuMV) is the primary pathogen. In recent years, some research progress has been made in genetic analysis, mapping, cloning, and molecular mechanism elucidation of TuMV resistance genes, which lay the foundation for breeding disease-resistant and high-quality new varieties. We reviewed the inheritance analysis, resistance genes and molecular markers, bioinformatics, omics analysis, and the application of genetic engineering technology involved in TuMV resistance. We also made suggestions for future work.

Key words: Brassica rapa, turnip mosaic virus, inheritance analysis, resistance genes, research progress

吕善武, 张昌伟, 侯喜林, 邓书林. 白菜类作物抗TuMV研究进展. 植物学报, 2023, 58(2): 335-346.

Shanwu Lü, Changwei Zhang, Xilin Hou, Shulin Deng. Research Progress of TuMV Resistance for Brassica rapa. Chinese Bulletin of Botany, 2023, 58(2): 335-346.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB22061

https://www.chinbullbotany.com/CN/Y2023/V58/I2/335

图/表 2

参考文献 95

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抗性基因	植物材料	抗性品种	遗传/克隆情况	TuMV株系	参考文献
显性基因/标记
ConTR01	大白菜	RLR22	定位于R04, 候选基因可能是eIF(iso)4E	1、3、4、7、 8、9和12	Rusholme et al., 2007
Tu1-Tu4	大白菜	91-112	4个QTLs, Tu1位于LG5连锁群, Tu2位于LG10连锁群, Tu3位于LG3连锁群, Tu4位于LG4连锁群	C4	Zhang et al., 2008
TuR1-TuR4	大白菜	A52-2	TuR1和TuR2位于LG3连锁群, TuR3和TuR4位于LG7连锁群	C3	张俊华等, 2008
Tu1-Tu3	大白菜	Y195-93	Tu1位于R03连锁群, Tu2位于R04连锁群, Tu3位于R06连锁群	C4	张晓伟等, 2009
Rnt1-1	大白菜	AS9	定位于R06连锁群, 与内源抗坏血酸和脱氢抗坏血酸合成相关	UK1	Fujiwara et al., 2011, 2016
TuRBCH01	不结球白菜	Q048	定位于R06连锁群	C5	Wang et al., 2011
TuRB01b	大白菜	TD34-S1	定位于A06染色体, 与油菜TuRB01位于同一位点或互为等位基因	UK1	Lydiate et al., 2014
TuRB07	大白菜	VC1/VC40	定位于A06染色体, 候选基因是Bra018863, 属于CC-NBS-LRR基因	C4	Jin et al., 2014
TuMV-R	大白菜	VC40	定位于A06染色体的0.34 Mb区域, 有6个候选基因, 4个CC-NBS-LRRs基因, 2个病程相关基因	C4	Chung et al., 2014
TuRBCS01	大白菜	8407	定位于A04染色体的1.98 Mb区域	C4	Li et al., 2015
隐性基因/标记
retr01	大白菜	RLR22	定位于R04连锁群, 候选基因可能是eIF(iso)4E	1、3、4、7、 8、9和12	Rusholme et al., 2007
retr02	大白菜	BP8407	定位于A04染色体, 候选基因是Bra035393, 编码eIF(iso)4E蛋白	C4	Qian et al., 2013
trs	大白菜	SB18/SB22	定位于A04染色体, 可能与retr02紧密连锁或是其等位基因	CHN2、CHN3、CHN4和CHN5	Kim et al., 2013
retrcs03	大白菜	73	定位于A04染色体, 候选基因是Bra032679, 编码γ-干扰素诱导蛋白	C4	满卫萍, 2020
其它候选基因
BcTuR1	不结球白菜	短白梗	抗性候选基因, 受TuMV诱导高表达	C4	马景蕃等, 2009
BcTuR3	不结球白菜	短白梗	抗性候选基因, 受TuMV诱导高表达, 属于TIR- NB-LRR基因	C4	Ma et al., 2010
BcLRK01	不结球白菜	泰三抗	抗性候选基因, 受TuMV诱导高表达, 属于LRR- RLKs基因	C4	彭海涛等, 2012b
NhccGR1	不结球白菜	泰三抗	抗性候选基因, 受TuMV诱导高表达, 属于谷胱甘肽还原酶基因	C4	李彦肖等, 2012
BcTFIIIA	不结球白菜	NHCC001	TuMV抗性基因, 编码锌指转录因子	C4	Zhang et al., 2022b

抗性基因	植物材料	抗性品种	遗传/克隆情况	TuMV株系	参考文献
显性基因/标记
ConTR01	大白菜	RLR22	定位于R04, 候选基因可能是eIF(iso)4E	1、3、4、7、 8、9和12	Rusholme et al., 2007
Tu1-Tu4	大白菜	91-112	4个QTLs, Tu1位于LG5连锁群, Tu2位于LG10连锁群, Tu3位于LG3连锁群, Tu4位于LG4连锁群	C4	Zhang et al., 2008
TuR1-TuR4	大白菜	A52-2	TuR1和TuR2位于LG3连锁群, TuR3和TuR4位于LG7连锁群	C3	张俊华等, 2008
Tu1-Tu3	大白菜	Y195-93	Tu1位于R03连锁群, Tu2位于R04连锁群, Tu3位于R06连锁群	C4	张晓伟等, 2009
Rnt1-1	大白菜	AS9	定位于R06连锁群, 与内源抗坏血酸和脱氢抗坏血酸合成相关	UK1	Fujiwara et al., 2011, 2016
TuRBCH01	不结球白菜	Q048	定位于R06连锁群	C5	Wang et al., 2011
TuRB01b	大白菜	TD34-S1	定位于A06染色体, 与油菜TuRB01位于同一位点或互为等位基因	UK1	Lydiate et al., 2014
TuRB07	大白菜	VC1/VC40	定位于A06染色体, 候选基因是Bra018863, 属于CC-NBS-LRR基因	C4	Jin et al., 2014
TuMV-R	大白菜	VC40	定位于A06染色体的0.34 Mb区域, 有6个候选基因, 4个CC-NBS-LRRs基因, 2个病程相关基因	C4	Chung et al., 2014
TuRBCS01	大白菜	8407	定位于A04染色体的1.98 Mb区域	C4	Li et al., 2015
隐性基因/标记
retr01	大白菜	RLR22	定位于R04连锁群, 候选基因可能是eIF(iso)4E	1、3、4、7、 8、9和12	Rusholme et al., 2007
retr02	大白菜	BP8407	定位于A04染色体, 候选基因是Bra035393, 编码eIF(iso)4E蛋白	C4	Qian et al., 2013
trs	大白菜	SB18/SB22	定位于A04染色体, 可能与retr02紧密连锁或是其等位基因	CHN2、CHN3、CHN4和CHN5	Kim et al., 2013
retrcs03	大白菜	73	定位于A04染色体, 候选基因是Bra032679, 编码γ-干扰素诱导蛋白	C4	满卫萍, 2020
其它候选基因
BcTuR1	不结球白菜	短白梗	抗性候选基因, 受TuMV诱导高表达	C4	马景蕃等, 2009
BcTuR3	不结球白菜	短白梗	抗性候选基因, 受TuMV诱导高表达, 属于TIR- NB-LRR基因	C4	Ma et al., 2010
BcLRK01	不结球白菜	泰三抗	抗性候选基因, 受TuMV诱导高表达, 属于LRR- RLKs基因	C4	彭海涛等, 2012b
NhccGR1	不结球白菜	泰三抗	抗性候选基因, 受TuMV诱导高表达, 属于谷胱甘肽还原酶基因	C4	李彦肖等, 2012
BcTFIIIA	不结球白菜	NHCC001	TuMV抗性基因, 编码锌指转录因子	C4	Zhang et al., 2022b