花粉外被蛋白B类小肽, 花粉打开柱头大门的一把钥匙

doi:10.11983/CBB21048

植物学报 ›› 2021, Vol. 56 ›› Issue (2): 147-150.DOI: 10.11983/CBB21048 cstr: 32102.14.CBB21048

花粉外被蛋白B类小肽, 花粉打开柱头大门的一把钥匙

王伟, 孙蒙祥()

武汉大学生命科学学院, 武汉 430072

收稿日期:2021-03-15 接受日期:2021-03-24 出版日期:2021-03-01 发布日期:2021-04-09
通讯作者: 孙蒙祥
作者简介:^*E-mail: mxsun@whu.edu.cn
基金资助:
国家自然科学基金(31800264)

POLLENCOAT PROTEIN B-class Peptides (PCP-Bs), a Key of Compatible Pollen to Open the Gate of Stigma

Wei Wang, Mengxiang Sun()

College of Life Sciences, Wuhan University, Wuhan 430072, China

Received:2021-03-15 Accepted:2021-03-24 Online:2021-03-01 Published:2021-04-09
Contact: Mengxiang Sun

摘要/Abstract

摘要： 植物有性生殖过程中, 花粉-柱头间的识别作用是确保亲和花粉萌发、完成受精并保持后代遗传稳定性的重要环节, 也是农业生产上杂交育种的一个障碍。相关研究历来备受关注。然而, 经过几十年的研究, 亲和花粉如何被识别仍是未解之谜。最近, 华东师范大学李超团队的研究成果, 揭示花粉外被B类小肽PCP-Bs与柱头分泌的RALF23/33小肽竞争性地与柱头乳突细胞质膜上的ANJ-FER受体激酶复合体直接结合, 通过下游RAC/ROP-NADPH氧化酶信号途径调节柱头活性氧水平, 从而调节亲和花粉水合作用的机制。这一发现是认识花粉与柱头识别机制的重要突破。

关键词: 花粉-柱头识别, 花粉外被蛋白, ANJ-FER受体激酶复合体, 小肽, 活性氧

Abstract: During sexual plant reproduction, pollen-stigma recognition is a critical step for the germination of compatible pollen to ensure successful fertilization and genetic stability of offspring. It is also the first barrier for interspecific hybridization in crop breeding. Thus, great efforts have been made in relevant investigations during past decades. However, how the compatible pollen is recognized by the stigma remains a mystery. Recently, Chao Li’s group from East China Normal University published their work inScience, which reveals that POLLEN COAT PROTEIN B-class peptides (PCP-Bs) could compete with stigma RALF23/33 for binding to the ANJ-FER complex on stigma surface, resulting in a decline of stigmatic reactive oxygen species (ROS) that facilitates compatible pollen hydration. This finding represents a breakthrough in the field.

Key words: pollen-stigma recognition, pollen coat protein, ANJ-FER receptor kinase complex, peptide, reactive oxygen species (ROS)

王伟, 孙蒙祥. 花粉外被蛋白B类小肽, 花粉打开柱头大门的一把钥匙. 植物学报, 2021, 56(2): 147-150.

Wei Wang, Mengxiang Sun. POLLENCOAT PROTEIN B-class Peptides (PCP-Bs), a Key of Compatible Pollen to Open the Gate of Stigma. Chinese Bulletin of Botany, 2021, 56(2): 147-150.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB21048

https://www.chinbullbotany.com/CN/Y2021/V56/I2/147

图/表 1

图1 拟南芥花粉与柱头的相互作用 1: 花粉落到柱头上; 2, 3: 花粉黏附与水合; 4: 花粉萌发。花粉黏附到柱头上之后, 形成花粉足(红色部分), 花粉足是花粉与柱头之间信号交流的通道。目前已知在花粉中参与水合的因子包括花粉外壁脂质以及相关的脂质酶、脂质结合蛋白和PCP-Bs小肽等。柱头中涉及水合的因子包括钙离子和Exo 70A1 (囊泡运输)等。

Figure 1 Pollen-stigma interaction in Arabidopsis 1: Pollen landing on the stigma; 2, 3: Pollen adhesion and hydration; 4: Pollen germination. After pollen adhesion a foot structure (red) established, which bridges pollen and papillar cells for the signaling between them. Currently, the known pollen factors involved in its hydration include pollen exine lipid and relevant lipidase, lipid binding proteins, and PCP-Bs, etc. The stigma factors involved in the hydration include cal-cium ion and Exo70A1 (vesicle trafficking), etc.

参考文献 13

[1]	Cabrillac D, Cock JM, Dumas C, Gaude T (2001). The S-locus receptor kinase is inhibited by thioredoxins and activated by pollen coat proteins. Nature 410,220-223.
[2]	Chapman LA, Goring DR (2010). Pollen-pistil interactions regulating successful fertilization in the Brassicaceae. J Exp Bot 61, 1987-1999.
[3]	Doucet J, Lee HK, Goring DR (2016). Pollen acceptance or rejection: a tale of two pathways. Trends Plant Sci 21,1058-1067.
[4]	Heslop-Harrison J (1975). Incompatibility and the pollen- stigma interaction. Annu Rev Plant Physiol 26,403-425.
[5]	Heslop-Harrison Y (1977). The pollen-stigma interaction: pollen-tube penetration in Crocus. Ann Bot 41,913-922.
[6]	Lin Z, Eaves DJ, Sanchez-Moran E, Franklin FCH, Frank-alin-Tong VE (2015). The Papaver rhoeas S determinants confer self-incompatibility to Arabidopsis thaliana in plan- ta. Science 350,684-687.
[7]	Liu C, Shen L, Xiao Y, Vyshedsky D, Peng C, Sun X, Liu Z, Cheng L, Zhang H, Han Z, Chai J, Wu HM, Cheung AY, Li C (2021). PCP-B peptides unlock a stigma 1 pep-tide-receptor kinase gated mechanism for pollination. Science doi: 10.1126/science.abc6107
[8]	Mattsson O, Knox RB, Heslop-Harrison J, Heslop-Harri-son Y (1974). Protein pellicle of stigmatic papillae as a probable recognition site in incompatibility reactions. Nature 247,298-300.
[9]	Nasrallah ME, Liu P, Nasrallah JB (2002). Generation of self-incompatible Arabidopsis thaliana by transfer of two S locus genes from A. lyrata. Science 297,247-249.
[10]	Schopfer CR, Nasrallah ME, Nasrallah JB (1999). The male determinant of self-incompatibility in Brassica. Science 286, 1697-1700.
[11]	Takayama S, Shiba H, Iwano M, Shimosato H, Che FS, Kai N, Watanabe M, Suzuki G, Hinata K, Isogai A (2000). The pollen determinant of self-incompatibility in Brassica campestris. Proc Natl Acad Sci USA 97, 1920- 1925.
[12]	Wang LD, Clarke LA, Eason RJ, Parker CC, Qi BX, Scott RJ, Doughty J (2017). PCP-B class pollen coat proteins are key regulators of the hydration checkpoint in Arabidopsis thaliana pollen-stigma interactions. New Phytol 213,764-777.
[13]	Wheeler MJ, de Graaf BHJ, Hadjiosif N, Perry RM, Poulter NS, Osman K, Vatovec S, Harper A, Franklin FCH, Franklin-Tong VE (2009). Identification of the pollen self-incompatibility determinant in Papaver rhoeas. Nature 459,992-995.

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花粉外被蛋白B类小肽, 花粉打开柱头大门的一把钥匙

POLLENCOAT PROTEIN B-class Peptides (PCP-Bs), a Key of Compatible Pollen to Open the Gate of Stigma

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