植物学报 ›› 2020, Vol. 55 ›› Issue (4): 421-429.DOI: 10.11983/CBB19244

• 研究报告 • 上一篇    下一篇

拟南芥AtR8 lncRNA对盐胁迫响应及其对种子萌发的调节作用

张楠1,刘自广2,孙世臣3,刘圣怡4,林建辉1,彭疑芳5,张晓旭1,杨贺1,岑曦1,吴娟1,*()   

  1. 1东北林业大学生命科学学院, 东北盐碱植被恢复与重建教育部重点实验室, 哈尔滨 150040
    2黑龙江省农业科学院畜牧研究所, 哈尔滨 150028
    3黑龙江省农业科学院耕作栽培研究所, 哈尔滨 150028
    4牡丹江医学院, 牡丹江 1570115东北农业大学, 哈尔滨 150030
  • 收稿日期:2019-12-18 接受日期:2020-04-15 出版日期:2020-07-01 发布日期:2020-05-21
  • 通讯作者: 吴娟
  • 基金资助:
    黑龙江省科学基金(LH2019C007);中央高校基本科研业务费专项资金(2572019CT03);东北油田盐碱植被恢复与重建教育部重点实验室开放基金(SAVER1702)

Response of AtR8 lncRNA to Salt Stress and Its Regulation on Seed Germination in Arabidopsis

Nan Zhang1,Ziguang Liu2,Shichen Sun3,Shengyi Liu4,Jianhui Lin1,Yifang Peng5,Xiaoxu Zhang1,He Yang1,Xi Cen1,Juan Wu1,*()   

  1. 1Key Laboratory of Northeast Salinity and Vegetation Restoration and Reconstruction, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, China
    2Institute of Animal Science, Heilongjiang Academy of Ag-ricultural Sciences, Harbin 150028, China
    3Institute of Farming and Cultivation, Heilongjiang Academy of Agricultural Sciences, Harbin 150028, China
    4Mudanjiang Medical University, Mudanjiang 157011, China
  • Received:2019-12-18 Accepted:2020-04-15 Online:2020-07-01 Published:2020-05-21
  • Contact: Juan Wu

摘要: 长链非编码RNA (lncRNA)是一类长度大于200个核苷酸且不编码蛋白质的非编码RNA, 主要由RNA聚合酶II转录生成, 大量存在于生物体内并具有多种生物学功能。AtR8 lncRNA是拟南芥(Arabidopsis thaliana)中RNA聚合酶III转录的长链非编码RNA。前期研究发现, 水杨酸(SA)处理诱导萌发种子中AtR8 lncRNA的表达, AtR8 lncRNA缺失抑制SA胁迫下的种子萌发。进一步研究发现, AtR8 lncRNA转录区域内存在保守的盐胁迫响应元件(TCTTCTTCTTTA); NaCl处理抑制萌发种子中AtR8 lncRNA的表达; 与野生型相比, 高浓度NaCl处理明显抑制了atr8 (AtR8 lncRNA部分缺失型拟南芥)种子萌发。研究结果表明, AtR8 lncRNA在拟南芥种子萌发期的盐胁迫中起重要作用。

关键词: 长链非编码RNA, 拟南芥, 种子萌发, 盐胁迫

Abstract: Long non-coding RNA (lncRNA) is a type of non-coding RNA that is longer than 200 nucleotides and does not encode proteins. lncRNAs are mainly produced by the transcription of RNA polymerase II and are abundant in the organism and have various biological functions. AtR8 lncRNA is transcribed by RNA polymerase III in Arabidopsis thaliana. Previous studies revealed that Salicylic acid (SA) induces AtR8 lncRNA expression in germinated seeds and that the deletion of AtR8 lncRNA decreases seed germination under SA stress. In this study, we found a conserved salt-stress-responsive element (TCTTCTTCTTTA) in the transcriptional region of AtR8 lncRNA. NaCl treatment inhibited AtR8 lncRNA expression in the germinated seeds. High concentration of NaCl significantly inhibited seed germination of atr8, which had partial deletion of AtR8 lncRNA, compared to that of the wild type, indicating that AtR8 lncRNA plays an important role in regulating seed germination in response to salt stress.

Key words: long non-coding RNA, Arabidopsis thaliana, seed germination, salt stress