植物学报 ›› 2019, Vol. 54 ›› Issue (5): 634-641.DOI: 10.11983/CBB18193

• 技术方法 • 上一篇    下一篇

不同激素对青钱柳外植体和愈伤组织褐化的影响

冯莹1,*(),钱莲文1,林庆良2   

  1. 1. 泉州师范学院资源与环境学院, 泉州 362000
    2, 福建农林大学, 福州 350002
  • 收稿日期:2018-09-11 接受日期:2019-02-19 出版日期:2019-09-01 发布日期:2020-03-10
  • 通讯作者: 冯莹
  • 基金资助:
    福建省科技厅引导性项目(2017N0028);泉州市科技计划(No.2018N007);泉州市科技计划(No.2016N002);泉港区科技计划(No.2016N06)

The Effect of Different Hormones on Explant Browning and Callus Browning in Cyclocarya paliurus

Ying Feng1,*(),Lianwen Qian1,Qingliang Lin2   

  1. 1. School of Resource and Environmental Science, Quanzhou Normal University, Quanzhou 362000, China
    2. Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2018-09-11 Accepted:2019-02-19 Online:2019-09-01 Published:2020-03-10
  • Contact: Ying Feng

摘要: 以青钱柳(Cyclocarya paliurus)叶片为研究材料, 采用单因素完全随机试验法比较不同激素(6-BA、GA3和NAA)浓度组合对叶片外植体褐化的影响; 在此基础上, 分别采用二因素、单因素完全随机试验法比较6-BA+NAA不同浓度组合、不同基本培养基对愈伤组织褐化的影响。结果表明, 改良MS+1.0 mg∙L -1 6-BA+1.0 mg∙L -1 GA3+0.3 mg∙L -1 NAA是抑制青钱柳叶片外植体褐化的最佳培养基, 其褐化率为0, 愈伤组织诱导率100%; 改良MS+0.5 mg∙L -1 6-BA+0.2 mg∙L -1 NAA是抑制愈伤组织褐化的最佳培养基, 其褐化率为0, 愈伤组织增殖倍数达4.80倍, 愈伤组织呈黄绿色或黄色, 颗粒状, 颗粒小而紧密、质硬且表面干燥。该方法有效解决了青钱柳叶片外植体和愈伤组织褐化问题, 为青钱柳组织培养褐化控制提供了一条简单高效的离体培养途径, 也为青钱柳叶片离体再生体系的建立奠定基础。

关键词: 褐化, 青钱柳, 愈伤组织, 诱导, 增殖

Abstract: To determine the effect of different hormones on explant browning and callus browning in Cyclocarya paliurus, leaf browning was controlled on medium with different kinds and content of hormones (6-BA, GA3, NAA) by a single-factor completely random test. Callus browning was controlled on the medium with different content of 6-BA+NAA and different basic medium by a double- and single-factor completely random test, respectively. The rate of leaf browning reached 0, and the rate of callus induction was up to 100% on the improved MS medium with 1.0 mg∙L -1 6-BA and 1.0 mg∙L -1 GA3 and 0.3 mg∙L -1 NAA. The rate of callus browning reached 0, and the callus propagation was up to 4.80 times on the improved MS medium with 0.5 mg∙L -1 6-BA and 0.2 mg∙L -1NAA. The callus was yellow with green or yellow, granular, small and compact, hard and dry. This system effectively solved the problem of leaf browning and callus browning and provided a simple and effective way for tissue culture and a solid foundation for the regeneration system from leaf of C. paliurus.

Key words: browning, Cyclocarya paliurus, callus, induction, propagation