水稻类钙调磷酸酶亚基B蛋白质在叶片生长和白叶枯病抗性反应中的表达

doi:10.3724/SP.J.1259.2012.00483

植物学报 ›› 2012, Vol. 47 ›› Issue (5): 483-490.DOI: 10.3724/SP.J.1259.2012.00483 cstr: 32102.14.SP.J.1259.2012.00483

水稻类钙调磷酸酶亚基B蛋白质在叶片生长和白叶枯病抗性反应中的表达

刘雨萌¹, 兰金苹¹, 曹英豪², 刘钊¹, 刘丽娟¹, 李莉云¹, 曹振伟¹, 刘国振^1*

¹河北农业大学生命科学学院, 保定 071001;
²中国科学院北京基因组研究所, 北京 101318

收稿日期:2011-12-16 修回日期:2012-05-01 出版日期:2012-09-01 发布日期:2012-07-24
通讯作者: 刘国振
基金资助:
MAPK 基因在Xa21 介导的水稻白叶枯病抗性反应中的功能

Expression of Calcineurin B-like Proteins in Rice Leaves and During Interaction Between Rice and Xanthomonas oryzae pv. oryzae

Yumeng Liu¹, Jinping Lan¹, Yinghao Cao², Zhao Liu¹, Lijuan Liu¹, Liyun Li¹, Zhenwei Cao¹, Guozhen Liu^1*

¹College of Life Sciences, Hebei Agricultural University, Baoding 071001, China

²Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101318, China

Received:2011-12-16 Revised:2012-05-01 Online:2012-09-01 Published:2012-07-24
Contact: Guozhen Liu

摘要/Abstract

摘要： 钙信号介导植物对多种外部刺激的反应并参与调控广泛的生理学过程。钙离子结合蛋白质, 如类钙调磷酸酶亚基B蛋白质(calcineurin B-like protein, CBL), 对感受和传递钙信号具有重要作用。根据基因组测序及注释分析, 水稻(Oryza sativa)基因组中有10个CBL家族成员。采用基于抗体的蛋白质组学策略, 利用免疫印迹方法研究了水稻CBL蛋白质在叶片生长不同时期的表达, 揭示了其在正常发育过程中的表达模式。然后对Xa21介导的水稻白叶枯病抗性反应不同时间点的蛋白质表达进行检测, 发现OsCBL-1、OsCBL-3、OsCBL-5、OsCBL-9和OsCBL-10这5个蛋白质的表达发生了变化; 进一步比较它们在抗病、感病和对照处理中的表达情况, 发现其在不同反应间的表达也有区别, 提示了CBL蛋白质在水稻-白叶枯病菌互作过程中的功能。该研究为深入了解水稻CBL蛋白质的功能提供了有价值的线索。

关键词: 白叶枯病菌, 类钙调磷酸酶亚基B蛋白质, 水稻, Xa21

Abstract: Ca²⁺ ions play an essential role in various plant developmental processes and in plant response to environmental stimuli. Plants have evolved a diversity of unique proteins that bind Ca²⁺. Calcineurin B-like proteins (CBLs) represent one of the main classes of Ca²⁺ sensors in plants. There are 10 members of CBLs in rice genome. Here, we used western blot analysis to examine the expression patterns of CBLs in rice leaves at different developmental stages under normal growth conditions. We then investigated the expression of CBLs at different time-points in rice incompatible responses against Xanthomonas oryzae pv. oryzae (Xoo) mediated by bacterial blight-resistant gene Xa21. The expression of OsCBL-1, -3, -5, -9 and -10 changed in incompatible responses. Furthermore, the expression of CBL proteins differed among compatible and incompatible interaction and mock controls, which suggests that CBL proteins may play roles in the interaction between rice and Xoo.

Key words: bacterial blight, calcineurin B-like protein, rice, Xa21

刘雨萌, 兰金苹, 曹英豪, 刘钊, 刘丽娟, 李莉云, 曹振伟, 刘国振. 水稻类钙调磷酸酶亚基B蛋白质在叶片生长和白叶枯病抗性反应中的表达. 植物学报, 2012, 47(5): 483-490.

Yumeng Liu, Jinping Lan, Yinghao Cao, Zhao Liu, Lijuan Liu, Liyun Li, Zhenwei Cao, Guozhen Liu. Expression of Calcineurin B-like Proteins in Rice Leaves and During Interaction Between Rice and Xanthomonas oryzae pv. oryzae. Chinese Bulletin of Botany, 2012, 47(5): 483-490.

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水稻类钙调磷酸酶亚基B蛋白质在叶片生长和白叶枯病抗性反应中的表达

Expression of Calcineurin B-like Proteins in Rice Leaves and During Interaction Between Rice and Xanthomonas oryzae pv. oryzae

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