Chin Bull Bot ›› 2019, Vol. 54 ›› Issue (3): 378-384.doi: 10.11983/CBB18181

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Tissue Culture and Rapid Propagation of Ardisia gigantifolia

Tang Fengluan,Zhao Jian,Zhao Zhiguo,Xia Ke,Qiu Shuo()   

  1. Guangxi Institute of Botany, Chinese Academy of Sciences and Guangxi Zhuangzu Autonomous Region, Guilin 541006, China
  • Received:2018-08-22 Accepted:2018-12-29 Online:2019-11-24 Published:2019-05-01
  • Contact: Qiu Shuo E-mail:qiushuo001@163.com

Abstract:

In this research, to establish a tissue culture and rapid propagation system of Ardisia gigantifolia, tender stem segments was selected as explants, and axillary buds were used to study proliferation. Culture medium of MS+1.0 mg·L -16-BA+0.2 mg·L -1 NAA and MS+0.5 mg·L -1 ZT had a significant effect on axillary bud induction and subculture of previous generations; the induction rates were 89.3% and 85.7%, respectively. The best culture medium for the multiplication was MS+0.5 mg·L -1 6-BA+0.1 mg·L -1 ZT+0.1 mg·L -1 NAA, and the propagation coefficient was 4.3. The optimal medium for rooting was 1/2MS+1.5 mg·L -1 IAA+1.0 mg·L -1NAA; the rooting rate reached 92.3%. The roots developed well and plantlets grew vigorously. Plantlets were transplanted to garden soil:peat soil:perlite=3:1:1 (v/v/v), and 82% rooted plants survived. The study established a fast propagation system for A. gigantifolia that could be applied to factory production.

Key words: Ardisia gigantifolia, axillary bud, tissue culture

Figure 1

Axillary bud culture, root induction and transplantation of Ardisia gigantifolia(A) The induction of axillary buds; (B) The subculture of axillary buds; (C) The proliferation of buds; (D)-(G) The rooting of plantlets; (H) The transplantation of rooting seedlings. (A) Bar=1 cm, (B)-(H) Bars=2 cm"

Table 1

Design and result of orthogonal test on axillary buds propagation of Ardisia gigantifolia"

No. Plant growth regulator (mg·L-1) Average height
(cm)
Average propagation coefficient State of growth
6-BA ZT NAA
1 0.1 0.1 0 5.5 2.5 Buds grew generally; leaves were medium, dark red
2 0.1 0.2 0.1 8.0 3.5 Buds grew well; leaves were big, dark red
3 0.1 0.4 0.5 8.3 3.5 Buds grew well; leaves were big, dark red
4 0.1 0.8 1.0 6.5 3.0 Buds grew generally; leaves were big, dark red
5 0.5 0.1 0.1 8.6 4.3 Buds grew well; leaves were big, dark red
6 0.5 0.2 0 7.2 3.5 Buds grew well; leaves were medium, dark red
7 0.5 0.4 1.0 7.5 4.0 Buds grew well; leaves were big, dark red
8 0.5 0.8 0.5 6.5 3.2 Buds grew well; leaves were big, dark red
9 1.0 0.1 0.5 5.5 4.0 Buds grew generally; leaves were medium; there are two or four lateral buds
10 1.0 0.2 1.0 6.5 4.5 Buds grew well; leaves were medium; there are two or four lateral buds
11 1.0 0.4 0 4.8 3.5 Buds grew generally; leaves were small; there are one or three lateral buds
12 1.0 0.8 0.1 3.5 3.0 Buds grew generally; leaves were small; there are one or two lateral buds
13 2.0 0.1 1.0 3.0 2.5 Buds grew poorly; leaves were small and yellow; base of seedlings had many calli
14 2.0 0.2 0.5 3.0 1.8 Seedlings had variation; leaves were small and yellow; base of seedlings had many calli
15 2.0 0.4 0.1 2.0 1.5 Buds grew poorly; leaves were very small and some of them had fell off; base of seedlings had many calli
16 2.0 0.8 0 1.8 0.9 Buds grew poorly and died partially

Table 2

Analysis and comparison of average height and propagation coefficient of buds of Ardisia gigantifolia"

Average height Average propagation coefficient
6-BA ZT NAA 6-BA ZT NAA
Range analysis and multiple comparisons
K1 7.1 a 5.7 4.8 3.1 a 3.3 2.6
K2 7.4 a 6.2 5.5 3.8 a 3.3 3.1
K3 5.1 b 5.7 5.8 3.8 a 3.1 3.1
K4 2.5 c 4.8 5.9 1.7 b 2.5 3.5
Range 5.0 1.6 1.1 2.1 0.8 0.9
Variance analysis
Degrees of freedom 3 3 3 3 3 3
DEVSQ 63.1 5.4 2.8 11.5 1.7 1.7
F value 4.1* 0.4 0.2 3.7* 0.6 0.5
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