植物学报 ›› 2016, Vol. 51 ›› Issue (1): 9-15.doi: 10.11983/CBB14188

• 研究报告 • 上一篇    下一篇

AtCPL1调控拟南芥开花的机制

亓钰莹, 展妍丽, 王萃铂, 陈发棣, 蒋甲福*()   

  1. 南京农业大学园艺学院, 南京 210095
  • 收稿日期:2014-10-27 接受日期:2015-03-11 出版日期:2016-01-01 发布日期:2016-02-01
  • 通讯作者: 蒋甲福 E-mail:jiangjiafu@njau.edu.cn
  • 作者简介:

    ? 共同第一作者

  • 基金资助:
    基金项目: 国家自然科学基金(No.31372100)、教育部新世纪优秀人才支持计划(No;NCET-12-0890)、江苏省“青蓝工程”中青年学术带头人培养计划和江苏省“双创计划”人才资助

Mechanism of AtCPL1 in Regulating Flowering of Arabidopsis

Yuying Qi, Yanli Zhan, Cuibo Wang, Fadi Chen, Jiafu Jiang*   

  1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2014-10-27 Accepted:2015-03-11 Online:2016-01-01 Published:2016-02-01
  • Contact: Jiang Jiafu E-mail:jiangjiafu@njau.edu.cn
  • About author:

    ? These authors contributed equally to this paper

摘要:

RNA聚合酶II CTD磷酸化酶1 (CPL1)作为影响RNA聚合酶II磷酸化水平的重要因子, 在植物逆境响应、离子吸收以及成花诱导等生命过程中扮演重要角色。为深入探究CPL1参与植物开花时间调控的作用机制, 以拟南芥(Arabidopsis thaliana) AtCPL1突变体cpl1-3fry2-1为研究材料, 观察了长日照条件下突变体与野生型的开花时间, 并利用荧光定量PCR技术对突变体中开花相关基因的表达情况进行了检测。结果表明: 在长日照条件下, 突变体cpl1-3fry2-1抽薹时的莲座叶数目均显著多于野生型, 且表现出明显的开花时间延迟现象; 荧光定量PCR分析显示, 突变体cpl1-3fry2-1中开花抑制因子miR156aTOEsSMZ基因的表达量较野生型显著升高, 而开花促进因子FTFDCOmiR172基因的表达量则显著降低。由此推测, AtCPL1通过调控miR156miR172的表达水平进而影响下游开花相关基因TOEsSMZFTFDCO等的表达, 从而实现对拟南芥开花时间的调控。

Abstract:

CTD-phosphatase-like 1 (CPL1), the RNA polymerase II, plays an important role in plant stress responses, ion absorption and floral induction by regulating the phosphorylation of RNA polymerase II. To study the mechanism of CPL1 involved in regulation of flowering, two AtCPL1 mutants, cpl1-3 and fry2-1, were used for observing differences in flowering phenotype. Deletion of AtCPL1 caused flowering delay in Arabidopsis under long-day conditions. Moreover, the expression of genes related to flowering regulatory pathways in cpl1-3 and fry2-1 mutants were analyzed by qRT-PCR. The expression of FT, FD, CO and miR172 was significantly upregulated in cpl1-3 and fry2-1 mutants, but that of miR156a, TOEs and SMZ was significantly downregulated. AtCPL1 may be involved in the regulation of flowering in Arabidopsis as a positive regulatory factor, perhaps by regulating the expression of miR156 and miR172, which affect the expression of flowering-related genes.

表1

引物序列"

Primer name Sequence (5'-3') Annotation
cpl1-3-F TTGTCCTCATATTTGTGAGTTTTGG
cpl1-3-R CCTCAAATGAGCGCATGGTATT
fry2-1- F CTCAACTCAGTCTGACAGGATGCT
fry2-1-R TCCCAATACCCAAGTCAGTGATT
UBQ10-F GGCCTTGTATAATCCCTGATGAATAAG Liu et al., 2011
UBQ10-R AAAGAGATAACAGGAACGGAAACATAGT
FT-F AGACGTTCTTGATCCGTTTA Jung et al., 2007
FT-R GTAGATCTCAGCAAACTCGC
CO-F CCGGGTCTGCGAGTCATG Yu et al., 2012
CO-R GGCATCATCTGCCTCACACA
SOC1-F GGTTTCTGAAGAAAATATGCAGCATTTG Yang et al., 2010
SOC1-R GGCTACTCTCTTCATCACCTCTTCCAG
FD-F GCGCTAGGAAACAGGAATGCTTATACAAA Yang et al., 2010
FD-R AGCTGTGGAAGACCGTTGAAGTGTG
SMZ-F AGGGAGAAGGAGCCATGAAGTTTGGTG Yu et al., 2012
SMZ-R GTCTTCAGAGGTTTCATGGTTGCCATG
miR156a-F CTCAAGTTCATTGCCATTTTTAGG Wang et al., 2009
miR156a-R GAGAGATTGAGACATAGAGAACGAAGA
miR172a1-F GGGTTTGAGTTTGAGTTTGA Jung et al., 2007
miR172a1-R TGGATGGAATCCAAATAATC
miR172a2-F TCGGCGGATCCATGGAAGAAAGCTC Jung et al., 2007
miR172a2-R TTTCTCAAGCTTTAGGTATTTGTAG
miR172b1-F CCGTCTTGAGTCTTGAAAAG Jung et al., 2007
miR172b1-R GAAATACCTCCGATCTGTGA
TOE1-F CGAGTTATAATAATCCCGCCGAG Yu et al., 2012
TOE1-R TTAAGGGTGTGGATAAAAGT
TOE2-F ATGGAGAACCACATGGCTGC Yu et al., 2012
TOE2-R GGTGCTGTAGCTGCTACGGC
TOE3-F ACGAGGAACGGTCATAATCTTG Yu et al., 2012
TOE3-R ATTCCCACCACTCGATTTCCT
FLC-F AGCCAAGAAGACCGAACTCA Yu et al., 2012
FLC-R TTTGTCCAGCAGGTGACATC

图1

长日照条件下cpl1-3和fry2-1突变体的开花表型 (A), (B) 突变体cpl1-3开花时间; (C), (D) 突变体fry2-1开花时间; (E), (F) cpl1-3和fry2-1突变体开花时的莲座叶数。数据用平均值±标准差表示; *和**分别表示在P≤0.05和P≤0.01水平上差异显著。"

图2

长日照条件下cpl1-3 (A)和fry2-1 (B)突变体中开花相关基因的表达量 数据为平均值±标准差; *和**分别表示在P≤0.05和P≤0.01水平上差异显著。"

图3

AtCPL1参与拟南芥开花调控的可能途径"

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