Activation and Termination of Strigolactone Signal Perception in Rice

doi:10.11983/CBB24163

Abstract

Abstract: Strigolactone (SL) is a novel plant hormone that regulates important growth and developmental processes such as plant branching. In rice, the SL receptor D14 perceives SL signals, binds with the F-box protein D3, and recruits the transcriptional repressor D53, inducing the ubiquitination and degradation of D53, thereby triggering signal transduction and inhibiting tillering. A recent study discovered that nitrogen limitation induces SL biosynthesis in rice to activate the receptor D14, triggering SL signal transduction. Concurrently, nitrogen limitation also induces phosphorylation of the N-terminal disordered region (NTD) of D14, reducing the ubiquitination and degradation of receptor D14, thereby further enhancing SL perception. Through these two synergistic mechanisms, nitrogen limitation stimulates SL signal transduction, strongly inhibiting tillering and enabling rice to adapt to low nitrogen stress conditions. The study also found that the D14-D3 interaction induced by SL promotes the ubiquitination and degradation of D14, thereby mediating the termination of SL signal perception. These significant findings elucidate the mechanisms of activation and termination of SL perception in rice, revealing the crucial regulatory role of SL signals in controlling rice tillering under low nitrogen stress. This would provide key insights into plant adaptation to nutrient scarcity and guide the precise improvement of crop architecture and molecular breeding of rice for reduced fertilizer use and increased yield.

Key words: rice, strigolactone, receptor D14, nitrogen limitation, phosphorylation, ubiquitination

Ruifeng Yao, Daoxin Xie. Activation and Termination of Strigolactone Signal Perception in Rice[J]. Chinese Bulletin of Botany, 2024, 59(6): 873-877.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB24163

https://www.chinbullbotany.com/EN/Y2024/V59/I6/873

Figures/Tables 1

Figure 1 Model of activation and termination of strigolactone perception in rice Under nitrogen deficiency, rice induces the biosynthesis of strigolactone (SL), which binds to and induces a conformational change in the receptor D14 from an "open" to a "closed" pocket. This forms a complex with the F-box protein D3 and the transcriptional repressor D53, promoting the ubiquitination and degradation of D53, thereby activating SL signal transduction. Concurrently, nitrogen limitation induces phosphorylation of the N-terminal disordered region (NTD) of D14, reducing its ubiquitination and degradation, and further enhancing SL perception. Through these two synergistic mechanisms, low nitrogen signaling ensures the activation of SL signal transduction, inhibiting rice tillering. The SL-triggered D14-D3 interaction also promotes the ubiquitination and degradation of D14, thus mediating the termination of SL signal transduction.

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