Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (3): 385-393.DOI: 10.11983/CBB22077

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Cloning of Brassica napus EXA1 Gene and Its Regulation on Plant Disease Resistance

Nan Wu1, Lei Qin1, Kan Cui1, Haiou Li1, Zhongsong Liu2, Shitou Xia1()   

  1. 1Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
    2Hunan Branch of National Oilseed Crops Improvement, College of Agronomy, Hunan Agricultural University, Changsha 410128, China
  • Received:2022-04-17 Accepted:2022-07-03 Online:2023-05-01 Published:2023-05-17
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Abstract: Loss function of EXA1 in Arabidopsis thaliana has been shown to increase PR gene expression and enhance resistance to pathogens. Here, we cloned BnaEXA1 in Brassica napus and ectopically overexpressed in A. thaliana exa1-2 mutant. We found that overexpressed BnaEXA1 not only restored the morphological defects of the exa1-2 mutant, but also reduced the expression of PR1 and PR2, resulting in the susceptibility to Sclerotinia sclerotiorum and oomycetes H.a. Noco2 in exa1-2 mutant, indicating that BnaEXA1 plays an important role in regulating the basic resistance of plants.

Key words: Brassica napus, disease resistance, EXA1, PR gene expression, Sclerotinia sclerotiorum