Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (3): 373-384.DOI: 10.11983/CBB22063

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

AtGH3.17-mediated Regulation of Auxin and Brassinosteroid Response in Arabidopsis thaliana

Shuyao Zhou, Jianming Li, Juan Mao()   

  1. College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
  • Received:2022-04-05 Accepted:2022-07-25 Online:2023-05-01 Published:2023-05-17
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Abstract: Brassinosteroid and auxin play important roles in plant growth and development. There is a complex crosstalk between the two plant hormones. In order to study whether GH3.17 is involved in the crosstalk between auxin and brassinosteroid (BR), we created overexpression and CRISPR gene loss mutants of Arabidopsis GH3.17. The root length of GH3.17 CRISPR mutants was slightly longer than that of wild-type plants. However, the GH3.17 overexpression seedlings had typical auxin deficiency phenotypes with decreased height, curly leaves and shorten roots and petioles. The root length of GH3.17 overexpression seedlings was substantially increased by the application of low concentrations of indole acetic acid (IAA) or brassinolide (BL), and the overexpression plants were more sensitive to IAA and BR than the wild type plants. We also found that the expression of auxin signaling, and BR synthesis pathway-related genes IAA12 and IAA16 was inhibited in the overexpression plants, while the expression abundance of brassinosteroid biosynthetic genes DWF4 and CPD was increased, suggesting that the inhibition of auxin signal transduction caused by the deficiency of active auxin may be compensated by BR compounds accumulation.

Key words: auxin, brassinosteroid, early auxin-response gene, GH3.17