Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (6): 733-743.DOI: 10.11983/CBB18235

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Identifying Genes Responsive to Jasmonates in Apple Based on Transcriptome Analysis

Na Zhang1,2,Xiuxia Liu1,Xuesen Chen1,*(),Shujing Wu1,*()   

  1. 1 State Key Laboratory of Crop Biology, College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
    2 College of Biology and Brewing Engineering, Taishan University, Tai’an 271018, China
  • Received:2018-11-04 Accepted:2019-02-11 Online:2019-11-01 Published:2020-07-09
  • Contact: Xuesen Chen,Shujing Wu

Abstract: To study the molecular mechanism of resistance induced by exogenous methyljasmonate (MeJA) in apple, tissue cultured seedlings of Gala grown for 30 days were used as materials. The leaves were treated with 100 μmol∙L -1 MeJA for 12 h. RNA sequencing and bioinformatics analysis were conducted to identify differentially expressed genes induced by MeJA. Exogenous MeJA mainly affected the biosynthesis of sesquiterpenoids, triterpenoids and flavonoids in apple leaves and induced disease resistance by brassinolide signal transduction pathways indirectly. The key genes regulating sesquiterpenoid and triterpenoid biosynthesis and flavonoids biosynthesis are MDP0000702120 and MDP0000692178; MDP0000123379 was the key regulatory gene linked to the brassinosteroid signaling pathway and plant-pathogen interaction pathway.

Key words: jasmonates, disease resistance, signal transduction, transcriptome, apple