Chinese Bulletin of Botany ›› 2022, Vol. 57 ›› Issue (1): 42-55.DOI: 10.11983/CBB21158

• RESEARCH PAPER • Previous Articles     Next Articles

Identification and Mapping of a Rice Male Sterility Mutant ms102

Xia Wang1, Wei Yan1, Zhiqin Zhou2, Zhenyi Chang1, Minting Zheng1, Xiaoyan Tang1,2, Jianxin Wu1,*()   

  1. 1Guangdong Provincial Key Laboratory of Biotechnology for Plant Development/School of Life Sciences, South China Normal University, Guangzhou 510631, China
    2Shenzhen Institute of Molecular Crop Design, Shenzhen 518107, China
  • Received:2021-09-14 Accepted:2021-11-17 Online:2022-01-01 Published:2022-01-17
  • Contact: Jianxin Wu

Abstract: The recessive nuclear male sterility line is the base of the third-generation hybrid rice breeding technology. To explore new male sterility lines suitable for the breeding technology, we screened an ethyl methylsulfonate (EMS) mutant library derived from the elite xian variety Huanghuazhan (HHZ) and identified male sterility mutant 102 (ms102). The ms102 showed a normal vegetative development, but exhibited defective anther dehiscence and pollen abortion. Cytological analyses revealed that the degradation of the tapetum was abnormal in ms102, resulting in the abortion of microspores. Genetic analyses showed that the male sterile phenotype of ms102 was resulted from the mutation of a known male sterility gene Defective Pollen Wall 2 (DPW2), which encodes an acyl transferase. Thus, this study identified a recessive nuclear male sterility mutant, and further confirmed the function of DPW2 in rice pollen development.

Key words: rice, male sterility, gene cloning