Chinese Bulletin of Botany ›› 2013, Vol. 48 ›› Issue (2): 184-191.DOI: 10.3724/SP.J.1259.2013.00184
Previous Articles Next Articles
Ran Li†, Jiancai Li†, Guoxin Zhou, Yonggen Lou*
Received:
Revised:
Online:
Published:
Contact:
Supported by:
973
Abstract: Quantitative real-time PCR is popularly used to study gene transcription levels in plants. Validation of candidate reference genes is the key step to ensure relative quantification. Here, we selected 5 reference genes (eEF-1α, 18S rRNA, 25S rRNA, Actin, and UBQ5) and tested their expression stability in rice under various treatments. eEF-1α showed the most stable expression with mechanical wounding, 25S rRNA with stripped stem borer treatment, and Actin with leaf folder treatment. UBQ5 showed the most stable expression on treatment with 2 phloem-feeding herbivores, brown plant hopper and white-backed plant hopper. We validated the expression profiles of these candidate reference genes by studying that of OsHI-LOX. We offer an available reference for studying gene transcription levels with herbivore infestation in rice.
Ran Li, Jiancai Li, Guoxin Zhou, Yonggen Lou. Validation of Rice Candidate Reference Genes for Herbivore-induced Quantitative Real-time PCR Analysis[J]. Chinese Bulletin of Botany, 2013, 48(2): 184-191.
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.chinbullbotany.com/EN/10.3724/SP.J.1259.2013.00184
https://www.chinbullbotany.com/EN/Y2013/V48/I2/184