水稻响应缺铁的韧皮部汁液蛋白质组学分析

doi:10.11983/CBB18184

摘要/Abstract

摘要：

为鉴定水稻(Oryza sativa)响应缺铁的根冠长距离信号转导物质, 采用TMT标记技术分析了不同浓度铁处理下水稻韧皮部汁液的蛋白质组学变化, 共鉴定出206个差异蛋白, 其中54个蛋白表达丰度上调, 152个蛋白表达丰度下调。差异蛋白的KEGG通路分类主要包括激素信号代谢、谷胱甘肽代谢、碳代谢以及mRNA转运等代谢途径。此外, 对差异蛋白对应的生理指标进行测定, 发现激素、蔗糖、谷胱甘肽和转运蛋白等在缺铁条件下变化显著, 后续对这些差异蛋白的功能研究有助于揭示水稻响应铁素营养的长距离信号途径。

关键词: 水稻, 缺铁, 韧皮部, 长距离信号, TMT

Abstract:

In order to identify long-distance signals under iron (Fe) deficiency in rice (Oryza sativa), TMT label technique was used to study the protein profile of phloem sap under different iron concentrations. A total of 206 differentially ex- pressed proteins were identified: 54 were upregulated and 152 were downregulated. Most of these proteins are involved in hormone signal transduction, carbon metabolism, glutathione metabolism and mRNA transport. In addition, we mea- sured the physiological indicators according to the differentially expressed proteins. It was found that phytohormones, sucrose, glutathione and transporters were significantly changed under iron deficiency. To further study the function of these proteins can help to reveal the long distance signaling pathway of rice in response to Fe deficiency.

Key words: rice (Oryza sativa), iron deficiency, phloem, long-distance signal, TMT

陈琳,林焱,陈鹏飞,王绍华,丁艳锋. 水稻响应缺铁的韧皮部汁液蛋白质组学分析. 植物学报, 2019, 54(2): 194-207.

Lin Chen,Yan Lin,Pengfei Chen,Shaohua Wang,Yanfeng Ding. Effect of Iron Deficiency on the Protein Profile of Rice (Oryza sativa) Phloem Sap. Chinese Bulletin of Botany, 2019, 54(2): 194-207.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB18184

https://www.chinbullbotany.com/CN/Y2019/V54/I2/194

图/表 6

图1 水稻韧皮部汁液差异表达蛋白GO功能分类

Figure 1 Functional classification of the differentially expressed proteins of rice phloem sap via the Gene Ontology (GO) database

表1 缺铁处理下水稻韧皮部汁液差异表达蛋白KEGG通路分类

Table 1 Differentially expressed proteins in phloem exudates of rice under iron deficiency in KEGG pathway

Gene ID	Protein name	Unique peptides	FC (-Fe/+Fe)	P value
Plant hormone signal transduction
4332374	Probable protein phosphatase 2C 30	1	0.81	0.01
4333666	Regulatory protein NPR3 isoform X2	1	1.45	0.01
4337780	Probable indole-3-acetic acid-amido synthetase GH3.6	1	0.83	0.03
4340712	Gibberellin-regulated protein 5	1	1.22	0.00
Carbon metabolism
9268154	Malate synthase	12	0.77	0.03
4349723	Phosphoglycerate mutase-like protein 4	2	0.75	0.01
4345814	Alpha-amylase isozyme 3D	14	0.72	0.00
Stress and defense
4332474	L-ascorbate peroxidase 1, cytosolic	10	0.64	0.00
4336627	Probable phospholipid hydroperoxide glutathione peroxidase	2	0.75	0.00
4330235	Probable phospholipid hydroperoxide glutathione peroxidase	2	0.82	0.00
4349585	Cationic peroxidase 1	2	0.52	0.00
4347962	Peroxidase A2 isoform X2	8	0.80	0.00
4337232	Peroxidase 4	2	0.68	0.02
4332928	Peroxidase 2	1	0.58	0.00
4332175	Peroxidase A2	2	0.76	0.01
4344277	Peroxidase 2-like	5	0.82	0.00
4328425	4-hydroxyphenylpyruvate dioxygenase	5	0.82	0.02
Biosynthesis of amino acids
4342571	Tryptophan synthase alpha chain	1	0.79	0.05
Ribosome
4325555	50S ribosomal protein L13, chloroplastic	2	1.26	0.00
4324671	40S ribosomal protein S24-1	1	1.21	0.04
4334651	50S ribosomal protein L18, chloroplastic	2	1.21	0.04
3131445	Ribosomal protein S7 (plastid)	2	1.21	0.00
4342697	40S ribosomal protein S15a-1	1	1.28	0.02
3131440	Ribosomal protein S18 (plastid)	1	0.74	0.01
mRNA surveillance pathway
4347878	Polyadenylation and cleavage factor homolog 4	2	0.77	0.01
RNA transport
4329091	Eukaryotic translation initiation factor 1A	3	0.79	0.01
4345498	ABC transporter A family member 7	1	1.40	0.00

表1 缺铁处理下水稻韧皮部汁液差异表达蛋白KEGG通路分类

Table 1 Differentially expressed proteins in phloem exudates of rice under iron deficiency in KEGG pathway

Gene ID	Protein name	Unique peptides	FC (-Fe/+Fe)	P value
Plant hormone signal transduction
4332374	Probable protein phosphatase 2C 30	1	0.81	0.01
4333666	Regulatory protein NPR3 isoform X2	1	1.45	0.01
4337780	Probable indole-3-acetic acid-amido synthetase GH3.6	1	0.83	0.03
4340712	Gibberellin-regulated protein 5	1	1.22	0.00
Carbon metabolism
9268154	Malate synthase	12	0.77	0.03
4349723	Phosphoglycerate mutase-like protein 4	2	0.75	0.01
4345814	Alpha-amylase isozyme 3D	14	0.72	0.00
Stress and defense
4332474	L-ascorbate peroxidase 1, cytosolic	10	0.64	0.00
4336627	Probable phospholipid hydroperoxide glutathione peroxidase	2	0.75	0.00
4330235	Probable phospholipid hydroperoxide glutathione peroxidase	2	0.82	0.00
4349585	Cationic peroxidase 1	2	0.52	0.00
4347962	Peroxidase A2 isoform X2	8	0.80	0.00
4337232	Peroxidase 4	2	0.68	0.02
4332928	Peroxidase 2	1	0.58	0.00
4332175	Peroxidase A2	2	0.76	0.01
4344277	Peroxidase 2-like	5	0.82	0.00
4328425	4-hydroxyphenylpyruvate dioxygenase	5	0.82	0.02
Biosynthesis of amino acids
4342571	Tryptophan synthase alpha chain	1	0.79	0.05
Ribosome
4325555	50S ribosomal protein L13, chloroplastic	2	1.26	0.00
4324671	40S ribosomal protein S24-1	1	1.21	0.04
4334651	50S ribosomal protein L18, chloroplastic	2	1.21	0.04
3131445	Ribosomal protein S7 (plastid)	2	1.21	0.00
4342697	40S ribosomal protein S15a-1	1	1.28	0.02
3131440	Ribosomal protein S18 (plastid)	1	0.74	0.01
mRNA surveillance pathway
4347878	Polyadenylation and cleavage factor homolog 4	2	0.77	0.01
RNA transport
4329091	Eukaryotic translation initiation factor 1A	3	0.79	0.01
4345498	ABC transporter A family member 7	1	1.40	0.00

图2 缺铁条件下水稻韧皮部激素信号转导变化示意图红色表示蛋白丰度上调, 黄色表示蛋白丰度下调。实线表示直接作用, 虚线表示中间有省略过程。箭头表示促进作用(正效应), 短竖线表示抑制作用(负效应)。

Figure 2 Schematic representation of plant hormone signal transduction in phloem of rice under iron deficiencyRed represents an increase of protein expression abundance, and yellow represents a decrease. The solid lines indicate direct action and the dotted lines indicate omission process. The arrows mean a positive effect and the short vertical lines mean inhibited effect.

图3 缺铁处理3天后水稻地上部(A)和根系(B)激素含量变化不同小写字母表示缺铁(-Fe)和对照(+Fe)之间差异显著。

Figure 3 The hormone content in rice shoots (A) and roots (B) after three days of iron deficiency treatmentDifferent lowercase letters indicate significant differences between iron deficiency (-Fe) and the control (+Fe).

图4 缺铁条件下水稻韧皮部汁液碳代谢(A)和谷胱甘肽代谢(B)相关蛋白质丰度变化示意图黄色表示蛋白丰度下调。实线表示直接作用, 虚线表示中间有省略过程。箭头表示促进作用(正效应)。

Figure 4 Metabolic pathways of carbon (A) and glutathione (B) related proteins in phloem exudates of rice under iron deficiencyYellow represents a decrease of protein expression abundance. The solid lines indicate direct action and the dotted lines indicate omission process. The arrows mean a positive effect.

图5 水稻缺铁处理3天后根系OsIRT1、OsYSL15、OsNAS1和OsNAS2 (A)以及地上部OsYSL2和OsNAS1 (B)相对表达水平柱形图代表缺铁与对照基因相对表达量的比值。

Figure 5 The relative expression levels of OsIRT1, OsYSL15, OsNAS1 and OsNAS2 in root (A) and the relative expression levels of OsYSL2 and OsNAS1 in shoot (B) after three days of iron deficiency treatmentThe column diagram represents the relative gene expression level ratio of iron deficiency induced to the control.

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