一个新的水稻花器官突变体的鉴定和基因定位

doi:10.3724/SP.J.1259.2011.00506

植物学报 ›› 2011, Vol. 46 ›› Issue (5): 506-513.DOI: 10.3724/SP.J.1259.2011.00506 cstr: 32102.14.SP.J.1259.2011.00506

一个新的水稻花器官突变体的鉴定和基因定位

罗伟雄¹, 李明², 陈军¹, 罗琼^1*

¹云南农业大学农业生物多样性与病虫害控制教育部重点实验室/云南省植物病理重点实验室, 昆明 650201
²中国科学院遗传与发育生物学研究所, 植物基因组学国家重点实验室, 北京 100101

收稿日期:2011-01-27 修回日期:2011-04-25 出版日期:2011-09-01 发布日期:2018-12-06
通讯作者: 罗琼
基金资助:
国家自然科学基金(No.30760100/30900885)和云南省应用基础研究计划(No.2007C0063M)

Characterization and Gene Mapping of a Novel Mutant in Rice Floral Organs

Weixiong Luo¹, Ming Li², Jun Chen¹, Qiong Luo^1*

¹Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Ministry of Education/Key Laboratory of Plant Pathology, Yunnan Agricultural University, Kunming 650201, China;
²State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

Received:2011-01-27 Revised:2011-04-25 Online:2011-09-01 Published:2018-12-06
Contact: Qiong Luo

摘要/Abstract

摘要： 在水稻(Oryza sativa)品种台中65的组培后代中发现一个花器官发育异常突变体flower organ number 6 (fon6), 其主要表型为: 双子房, 多柱头, 7–8枚雄蕊。遗传分析表明, 该突变表型由一对隐性基因控制。以该突变体与籼稻3037杂交的F₂代分离群体作为定位群体, 利用STS标记将与突变性状相关的基因定位于第6染色体短臂上STS标记PL4和PL5之间约480 kb的范围内。该研究结果为进一步的基因克隆及功能研究奠定了基础。

关键词: 双子房, 花器官, 基因定位, 多柱头, 水稻

Abstract: We obtained a rice floral mutant, inner flower organ number 6 (fon6), from the progenies of tissue culture of a japonica variety Taichung65. The mutant has double ovaries, multi-stigmas, and 7 to 8 stamens. The mutant trait is controlled by a single recessive gene. The F₂ population derived from the cross between Zhongxian 3037 and fon6 was used for gene mapping. FON6 was mapped to the ~480-kb region between the sequence-tagged site markers PL4 and PL5 on chromosome 6. These results will be useful for cloning and functional analysis of the gene.

Key words: double-ovary, flower organ, gene mapping, multi-stigmas, rice

罗伟雄, 李明, 陈军, 罗琼. 一个新的水稻花器官突变体的鉴定和基因定位. 植物学报, 2011, 46(5): 506-513.

Weixiong Luo, Ming Li, Jun Chen, Qiong Luo. Characterization and Gene Mapping of a Novel Mutant in Rice Floral Organs. Chinese Bulletin of Botany, 2011, 46(5): 506-513.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.chinbullbotany.com/CN/10.3724/SP.J.1259.2011.00506

https://www.chinbullbotany.com/CN/Y2011/V46/I5/506

参考文献

计慎敏, 张大兵 (2007). 水稻花器官特征决定以及数量控制的分子机理. 植物学通报 24, 284–292.
张向前, 邹金, 朱海涛, 李晓燕, 曾瑞珍 (2008). 水稻早熟突变体fon5的遗传分析和基因定位．遗传30, 1349–1355.
Agrawal GK, Abe K, Yamazaki M, Miyao A and Hirochika H (2005). Conservation of the E-function for floral organ identity in rice revealed by the analysis of tissue culture-induced loss-of-function mutants of the OsMADS1 gene. Plant Mol Bio 59, 125–135.
Arora R, Agarwal P, Ray S, Singh AK, Singh VP, Tyagi AK and Kapoor S (2007). MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress. BMC Genomics 8, 242.
Chu HW, Qian Q, Liang WQ, Yin CS, Tan HX, Yao X, Yuan Z, Yang J, Huang H, Luo D, Ma H and Zhang DB (2006).The FLORAL ORGAN NUMBER4 gene encoding a putative ortholog of Arabidopsis CLAVATA3 regulates apical meristem size in rice. Plant Physiology 142 1039–1052.
Clark SE, Running MP, Meyerowitz EM (1993). CLAVATA1, a regulator of meristem and flower development in Arabidopsis. Development 119, 397-418.
Coen ES, Meyewitz EM (1991). The war of the whorls: genetic interactions controlling flower development. Natural 353, 31–37.
Coen ES, Romero JM, Doyle S, Elliott R, Murphy G and Carpenter R (1990). floricaula: A homeotic gene required for flower development in Antirrhinum majus. Cell 63, 1311–1322.
Colombo L, Franken J, Koetje E, Van Went J, Dons H, Angenent G and Van Tunen A (1995). The petunia MADS box gene FBP11 determines ovule identity. Plant Cell 7,1859–1868.
Fletcher JC, Brand U, Running MP, Simon R, Meyerowitz EM (1999). Signaling of cell fate decisions by CLAVATA3 in Arabidopsis shoot meristems. Science 283, 1911-1914.
Fornara F, Marziani GA, Mizzi L, Kater M and Colombo L (2003). MADS-box genes controlling flower development in rice. Plant Boil 5, 16–22.
Goto K, Meyerowitz EM (1994). Function and regulation of the Arabidopsis floral homeotic gene PISTILLATA. Genes Dev 8, 1548–1560.
Hong LL, Qian Q, Zhu KM, Tang D, Huang ZJ, Gao L, Li M, Gu MH and Cheng ZK (2010). ELE restrains empty glumes from developing into lemmas. J Genet Genomics 37, 101–115.
Jack T, Brochman LL, and Meyerowitz EM (1992). The homeotic gene APETALA3 of Arabidopsis thaliana encodes a MADS box and is expressed in petals and stamens. Cell 68, 683–697.
Jeon J, Lee S, Jung K, Yang W, Yi G, Oh B and An G (2000). Production of transgenic rice plants showing reduced heading date and plant height by ectopic expression of rice MADS-box genes. Mol Breed 6, 581–592.
Jiang L, Qian Q, Mao L, Zhou QY and Zhai WX (2005). Characterization of the rice floral organ number mutant fon3. Journal of Integrative Plant Biology 47, 100?106.
Lee S, Kim J, Son JS, Nam J, Jeong DH, Lee K, Jang S, Yoo J, Lee J, Lee DY, Kang HG, An G (2003). Systematic reverse genetic screening of T-DNA tagged genes in rice for functional genomic analyses: MADS-box genes as a test case. Plant Cell Physiol 44, 1403-1411.
Li HF, Liang WQ, Jia RD, Yin CS, Zong J and Zhang DB (2010). The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice. Cell Research 20, 299–313.
Li Y, Xu PZ, Zhang HY, Peng H, Zhang QF, Wang XD and Wu XJ (2007). Characterization and identification of a novel mutant fon(t) on floral organ number and floral organ identity in rice. J Genet Genomics 34, 730–737.
Lim J, Moon YH, An G and Jang S K (2000). Two rice MADS domain proteins interact with OsMADS1. Plant Mol Biol 44, 513–527.
Mandel MA, Gustafson-Brown C, Savidge B, and Yanofsky M (1992). Molecular characterization of the Arabidopsis floral homeotic gene APETALA1. Nature 360, 273–277.
Moon Y, Kang H, Jung J, Jeon J, Sung S and An G(1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiology 120, 1193–1204.
Murray MG, Thompson WF (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8, 4321–4326.
Nagasawa N, Miyoshi M, Sano Y, Satoh H, Sakai H and Nagato Y (2003). SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice. Development 130, 705–718.
Nam J, Kim J, Lee S, An G, Ma H and Nei M (2004). Type I MADS-box genes have experienced faster birth-and death evolution than II MADS-box genes in angiosperms. PNAS 101, 1910-1915.
Pelaz S, Ditta G, Baumann E, Wisman E and Yanofsky M (2000). B and C floral organ identity functions require SEPALLATA MADS-box genes. Nature 405, 200–203.
Sommer SZ, Huijser P, Nacken W, Saedler H and Sommer H (1990). Genetic control of flower development by homeotic genes in Antirrhinum majus. Science 250, 931–936.
Sun QW, Zhou DX (2008). Rice jmjC domain-containing gene JMJ706 encodes H3K9 demethylase required for floral organ development. PNAS 105, 13679–13684.
Suzaki T, Ohneda M, Toriba T, Yoshida A and Hirano HY (2009). FON2 SPARE1 redundantly regulates floral meristem maintenance with FLORAL ORGAN NUMBER2 in Rice. Plos Genetics 5, e1000693.
Suzaki T, Sato M, Ashikari M, Nagato Y and Hirano HY (2004). The gene FLORAL ORGAN NUMBER1 regulates floral meristem size in rice and encodes a leucine-rich repeat receptor kinase orthologous to Arabidopsis CLAVATA1. Development 131, 5649?5657.
Theissen G, Saedler H (2001). Floral quartets. Nature 409, 469–471.
Weigel D, Meyerowitz EM (1994). The ABCS of floral homeotic genes. Cell 78, 203–209.
Yamaguchi T, Hirano HY (2006). Function and diversification of MADS-box genes in rice. TSW Development & Embryology 1, 99–108.
Yanofsky M, Ma H, Bowman J, Drews G, Feldmann K and Meyerowitz E (1990). The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors. Nature 346, 35–39.

[1]	刘欣宇, 戴思兰. 生长素响应因子(ARFs)介导的植物花器官发育调控网络与作用机制[J]. 植物学报, 2026, 61(3): 369-385.
[2]	阳锦华, 王冰. 独脚金内酯受体精准改造助力水稻抗病毒“防卫战”[J]. 植物学报, 2026, 61(3): 357-368.
[3]	樊月玲, 蒋正德, 叶佳舒, 郑立臣, 陈欣. 2005-2015年下辽河平原农田长期观测样地主要农作物收获期性状和产量数据集[J]. 植物生态学报, 2025, 49(8): 1271-1282.
[4]	陈钧, 徐江民, 周逸楠, 江亚楠, 胡程翔, 金芊芸, 赵蓓蓓, 朱哲楠, 徐雨青, 张璐怡, 刘笑妍, 刘隽, 李三峰, 王跃星, 饶玉春. 水稻白叶枯病抗性QTL的挖掘与候选基因分析[J]. 植物学报, 2025, 60(5): 831-845.
[5]	江亚楠, 徐雨青, 魏毅铤, 陈钧, 张容菀, 赵蓓蓓, 林宇翔, 饶玉春. 水稻抗病调控机制研究进展[J]. 植物学报, 2025, 60(5): 734-748.
[6]	赵蔓雅, 孙倩楠, 徐晶晶, 段恬妮, 蔡锦涛, 周婧, 范婷婷, 萧浪涛, 王若仲. 一个新的黄瓜叶色突变体鉴定、初定位及转录组分析[J]. 植物学报, 2025, 60(4): 515-532.
[7]	叶灿, 姚林波, 金莹, 高蓉, 谭琪, 李旭映, 张艳军, 陈析丰, 马伯军, 章薇, 张可伟. 水稻水杨酸代谢突变体高通量筛选方法的建立与应用[J]. 植物学报, 2025, 60(4): 586-596.
[8]	赵凌, 管菊, 梁文化, 张勇, 路凯, 赵春芳, 李余生, 张亚东. 基于高密度Bin图谱的水稻苗期耐热性QTL定位[J]. 植物学报, 2025, 60(3): 342-353.
[9]	李新宇, 谷月, 徐非非, 包劲松. 水稻胚乳淀粉合成相关蛋白的翻译后修饰研究进展[J]. 植物学报, 2025, 60(2): 256-270.
[10]	李建国, 张怡, 张文君. 水稻根系铁膜形成及对磷吸收的影响[J]. 植物学报, 2025, 60(1): 132-143.
[11]	姚瑞枫, 谢道昕. 水稻独脚金内酯信号感知的激活和终止[J]. 植物学报, 2024, 59(6): 873-877.
[12]	连锦瑾, 唐璐瑶, 张伊诺, 郑佳兴, 朱超宇, 叶语涵, 王跃星, 商文楠, 傅正浩, 徐昕璇, 吴日成, 路梅, 王长春, 饶玉春. 水稻抗氧化性状遗传位点挖掘及候选基因分析[J]. 植物学报, 2024, 59(5): 738-751.
[13]	黄佳慧, 杨惠敏, 陈欣雨, 朱超宇, 江亚楠, 胡程翔, 连锦瑾, 芦涛, 路梅, 张维林, 饶玉春. 水稻突变体pe-1对弱光胁迫的响应机制[J]. 植物学报, 2024, 59(4): 574-584.
[14]	周俭民. 收放自如的明星战车[J]. 植物学报, 2024, 59(3): 343-346.
[15]	陈雯, 周颖盈, 罗平, 崔永一. 被子植物花朵重瓣化分子调控机制[J]. 植物学报, 2024, 59(2): 257-277.

一个新的水稻花器官突变体的鉴定和基因定位

Characterization and Gene Mapping of a Novel Mutant in Rice Floral Organs

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价