一个新的水稻花器官突变体的鉴定和基因定位

doi:10.3724/SP.J.1259.2011.00506

植物学报 ›› 2011, Vol. 46 ›› Issue (5): 506-513.DOI: 10.3724/SP.J.1259.2011.00506

一个新的水稻花器官突变体的鉴定和基因定位

罗伟雄¹, 李明², 陈军¹, 罗琼^1*

¹云南农业大学农业生物多样性与病虫害控制教育部重点实验室/云南省植物病理重点实验室, 昆明 650201
²中国科学院遗传与发育生物学研究所, 植物基因组学国家重点实验室, 北京 100101

收稿日期:2011-01-27 修回日期:2011-04-25 出版日期:2011-09-01 发布日期:2018-12-06
通讯作者: 罗琼
基金资助:
国家自然科学基金(No.30760100/30900885)和云南省应用基础研究计划(No.2007C0063M)

Characterization and Gene Mapping of a Novel Mutant in Rice Floral Organs

Weixiong Luo¹, Ming Li², Jun Chen¹, Qiong Luo^1*

¹Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Ministry of Education/Key Laboratory of Plant Pathology, Yunnan Agricultural University, Kunming 650201, China;
²State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

Received:2011-01-27 Revised:2011-04-25 Online:2011-09-01 Published:2018-12-06
Contact: Qiong Luo

摘要/Abstract

摘要： 在水稻(Oryza sativa)品种台中65的组培后代中发现一个花器官发育异常突变体flower organ number 6 (fon6), 其主要表型为: 双子房, 多柱头, 7–8枚雄蕊。遗传分析表明, 该突变表型由一对隐性基因控制。以该突变体与籼稻3037杂交的F₂代分离群体作为定位群体, 利用STS标记将与突变性状相关的基因定位于第6染色体短臂上STS标记PL4和PL5之间约480 kb的范围内。该研究结果为进一步的基因克隆及功能研究奠定了基础。

关键词: 双子房, 花器官, 基因定位, 多柱头, 水稻

Abstract: We obtained a rice floral mutant, inner flower organ number 6 (fon6), from the progenies of tissue culture of a japonica variety Taichung65. The mutant has double ovaries, multi-stigmas, and 7 to 8 stamens. The mutant trait is controlled by a single recessive gene. The F₂ population derived from the cross between Zhongxian 3037 and fon6 was used for gene mapping. FON6 was mapped to the ~480-kb region between the sequence-tagged site markers PL4 and PL5 on chromosome 6. These results will be useful for cloning and functional analysis of the gene.

Key words: double-ovary, flower organ, gene mapping, multi-stigmas, rice

罗伟雄, 李明, 陈军, 罗琼. 一个新的水稻花器官突变体的鉴定和基因定位. 植物学报, 2011, 46(5): 506-513.

Weixiong Luo, Ming Li, Jun Chen, Qiong Luo. Characterization and Gene Mapping of a Novel Mutant in Rice Floral Organs. Chinese Bulletin of Botany, 2011, 46(5): 506-513.

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链接本文: https://www.chinbullbotany.com/CN/10.3724/SP.J.1259.2011.00506

https://www.chinbullbotany.com/CN/Y2011/V46/I5/506

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一个新的水稻花器官突变体的鉴定和基因定位

Characterization and Gene Mapping of a Novel Mutant in Rice Floral Organs

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