不同光质的LED对蝴蝶兰组织培养增殖及生根的影响

doi:10.11983/CBB14196

植物学报 ›› 2016, Vol. 51 ›› Issue (1): 81-88.DOI: 10.11983/CBB14196

不同光质的LED对蝴蝶兰组织培养增殖及生根的影响

任桂萍^1,², 王小菁¹, 朱根发^2,^*()

¹华南师范大学生命科学学院, 广东省植物发育生物工程重点实验室, 广州 510631
²广东省农业科学院环境园艺研究所, 广东省园林花卉种质创新与利用重点实验室, 广州 510640

收稿日期:2014-11-15 接受日期:2015-04-14 出版日期:2016-01-01 发布日期:2016-02-01
通讯作者: 朱根发
作者简介:? 共同第一作者
基金资助:
基金项目: 广东省科技攻关重点项目(No.2011A020102007)和广东省战略新兴产业核心技术攻关项目(No.2012A020800003)

Effect of LED in Different Light Qualities on Growth of Phalaenopsis Plantlets

Guiping Ren^1,2, Xiaojing Wang¹, Genfa Zhu^2*

¹Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China
²Guangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences,Guangzhou 510640, China

Received:2014-11-15 Accepted:2015-04-14 Online:2016-01-01 Published:2016-02-01
Contact: Zhu Genfa
About author:? These authors contributed equally to this paper

摘要/Abstract

摘要： 以蝴蝶兰(Phalaenopsis ssp.)栽培种绿熊(Green Bear)和大辣椒(Big Chilli)为培养材料, 研究不同光质的发光二极管(LED)组合对组织培养过程中增殖及生根的影响。结果表明, 红光更有利于蝴蝶兰单芽增殖、干重、鲜重以及株高的增加, 但不利于叶片叶绿素的积累; 蓝光有利于叶片叶绿素的积累, 并能提高根系活力; 远红光则对根长和根系活力的增加作用更显著。增殖扩繁阶段的最适LED为暖白, 2种蝴蝶兰芽增殖系数分别比白色荧光灯(对照)高出53.17%和46.37%。生根诱导阶段的最适LED组合为红:蓝:远红=3:6:1, 根长及根系活力均较对照显著增加。该研究结果为LED光源在蝴蝶兰组织培养中的应用奠定了基础。

关键词: 发光二极管, 光质, 蝴蝶兰, 组织培养

Abstract: We used the aseptic seedlings of 2 cultivars (Phalaenopsis ssp. cv. ‘Green Bear’ and Phalaenopsis ssp. cv. ‘Big Chilli’) to investigate the effect of LED in different light qualities on multiplication and rooting in vitro. The experimental design included 15 different light sources composed of six kinds of LED light, namely RED(630), R(630)8B2, R(630)7B3, R(630)5B5, R(630)3B7, R(630)2B8, BLUE, RED(660), R(660)7B3, R(660)5B5, CW5R(630)5, Warm W, R(630)6B3FR1, R(630)4B4FR2, R(630)3B6FR1, and WFL (control). Under red light illumination, seeding growth and the number of shoots were promoted, but chlorophyll content of leaves was reduced, but it was increased under the mixture of blue and red LEDs. Blue light had a positive effect on chlorophyll content. Root activity was increased with increasing ratio of blue in the combined red and blue LEDs and increased under the three light sources supplemented with far-red light. Blue and far-red light were more effective in root length. Shoots could be more successfully induced under Warm W light. Compared with WFL light, the number of shoots of the 2 varieties was increased by 53.17% and 46.37%, respectively. Therefore, the best light recipe for the multiplication and rooting of Phalaenopsis were Warm W and R(630)3B6FR1 light. The present study provides a proof of application of LEDs in Phalaenopsis tissue culture.

Key words: light-emitting diode, light quality, Phalaenopsis, tissue culture

任桂萍, 王小菁, 朱根发. 不同光质的LED对蝴蝶兰组织培养增殖及生根的影响. 植物学报, 2016, 51(1): 81-88.

Guiping Ren, Xiaojing Wang, Genfa Zhu. Effect of LED in Different Light Qualities on Growth of Phalaenopsis Plantlets. Chinese Bulletin of Botany, 2016, 51(1): 81-88.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB14196

https://www.chinbullbotany.com/CN/Y2016/V51/I1/81

图/表 6

参考文献 18

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Light treatment	Factors	Wavelength
RED(630)	Red	630 nm
R(630)8B2	Red:Blue=8:2	630 nm/450-480 nm
R(630)7B3	Red:Blue=7:3	630 nm/450-480 nm
R(630)5B5	Red:Blue=5:5	630 nm/450-480 nm
R(630)3B7	Red:Blue=3:7	630 nm/450-480 nm
R(630)2B8	Red:Blue=2:8	630 nm/450-480 nm
BLUE	Blue	450-480 nm
RED(660)	Red	660 nm
R(660)7B3	Red:Blue=7:3	660 nm/450-480 nm
R(660)5B5	Red:Blue=5:5	660 nm/450-480 nm
CW5R(630)5	Cold white:Red=5:5	630 nm/400-780 nm
Warm W	Warm white LED	400 nm-780 nm (the range of 500-780 nm accounts for 91.80%)
R(630)6B3FR1	Red:Blue:Far red=6:3:1	630 nm/450-480 nm/730 nm
R(630)4B4FR2	Red:Blue:Far red=4:4:2	630 nm/450-480 nm/730 nm
R(630)3B6FR1	Red:Blue:Far red=3:6:1	630 nm/450-480 nm/730 nm
WFL	White fluorescent tube	360-750 nm

Light treatment	Factors	Wavelength
RED(630)	Red	630 nm
R(630)8B2	Red:Blue=8:2	630 nm/450-480 nm
R(630)7B3	Red:Blue=7:3	630 nm/450-480 nm
R(630)5B5	Red:Blue=5:5	630 nm/450-480 nm
R(630)3B7	Red:Blue=3:7	630 nm/450-480 nm
R(630)2B8	Red:Blue=2:8	630 nm/450-480 nm
BLUE	Blue	450-480 nm
RED(660)	Red	660 nm
R(660)7B3	Red:Blue=7:3	660 nm/450-480 nm
R(660)5B5	Red:Blue=5:5	660 nm/450-480 nm
CW5R(630)5	Cold white:Red=5:5	630 nm/400-780 nm
Warm W	Warm white LED	400 nm-780 nm (the range of 500-780 nm accounts for 91.80%)
R(630)6B3FR1	Red:Blue:Far red=6:3:1	630 nm/450-480 nm/730 nm
R(630)4B4FR2	Red:Blue:Far red=4:4:2	630 nm/450-480 nm/730 nm
R(630)3B6FR1	Red:Blue:Far red=3:6:1	630 nm/450-480 nm/730 nm
WFL	White fluorescent tube	360-750 nm

Light treatment	Green Bear			Big Chilli
Light treatment	Multiplication coefficient	Ratio (%) (number of shoot≥3)	Ratio (%) (number of shoot≥4)	Multiplication coefficient	Ratio (%) (number of shoot≥3)
RED(630)	2.85±0.16 c	51.39	25.00	2.20±0.14 g	34.55
R(630)8B2	2.26±0.13 a	38.46	11.54	1.85±0.11 cdef	18.52
R(630)7B3	2.33±0.14 a	36.84	13.16	1.78±0.12 bcdef	12.73
R(630)5B5	2.19±0.15 a	32.43	9.46	1.63±0.09 abcde	9.26
R(630)3B7	2.14±0.12 a	25.71	10.00	1.69±0.11 abcdef	14.55
R(630)2B8	2.37±0.11 a	36.90	11.90	1.50±0.08 abc	3.57
BLUE	2.27±0.13 a	33.33	14.67	1.66±0.13 abcdef	16.00
RED(660)	2.80±0.15 bc	56.25	25.00	2.00±0.12 fg	32.14
R(660)7B3	2.13±0.13 a	35.94	9.38	1.86±0.10 defg	21.57
R(660)5B5	2.39±0.14 ab	40.26	18.18	1.75±0.11 bcdef	19.23
CW5R(630)5	2.43±0.15 ab	38.67	20.00	1.94±0.12 efg	20.75
Warm W	3.14±0.17 c	62.16	36.49	2.02±0.12 fg	26.98
R(630)6B3FR1	2.32±0.17 a	34.78	14.49	1.49±0.09 ab	6.38
R(630)4B4FR2	2.29±0.19 a	31.82	13.64	1.56±0.09 abcd	8.33
R(630)3B6FR1	2.35±0.11 a	37.84	10.81	1.45±0.09 ab	5.45
WFL	2.05±0.12 a	22.08	6.49	1.38±0.07 a	1.82

Light treatment	Green Bear			Big Chilli
Light treatment	Multiplication coefficient	Ratio (%) (number of shoot≥3)	Ratio (%) (number of shoot≥4)	Multiplication coefficient	Ratio (%) (number of shoot≥3)
RED(630)	2.85±0.16 c	51.39	25.00	2.20±0.14 g	34.55
R(630)8B2	2.26±0.13 a	38.46	11.54	1.85±0.11 cdef	18.52
R(630)7B3	2.33±0.14 a	36.84	13.16	1.78±0.12 bcdef	12.73
R(630)5B5	2.19±0.15 a	32.43	9.46	1.63±0.09 abcde	9.26
R(630)3B7	2.14±0.12 a	25.71	10.00	1.69±0.11 abcdef	14.55
R(630)2B8	2.37±0.11 a	36.90	11.90	1.50±0.08 abc	3.57
BLUE	2.27±0.13 a	33.33	14.67	1.66±0.13 abcdef	16.00
RED(660)	2.80±0.15 bc	56.25	25.00	2.00±0.12 fg	32.14
R(660)7B3	2.13±0.13 a	35.94	9.38	1.86±0.10 defg	21.57
R(660)5B5	2.39±0.14 ab	40.26	18.18	1.75±0.11 bcdef	19.23
CW5R(630)5	2.43±0.15 ab	38.67	20.00	1.94±0.12 efg	20.75
Warm W	3.14±0.17 c	62.16	36.49	2.02±0.12 fg	26.98
R(630)6B3FR1	2.32±0.17 a	34.78	14.49	1.49±0.09 ab	6.38
R(630)4B4FR2	2.29±0.19 a	31.82	13.64	1.56±0.09 abcd	8.33
R(630)3B6FR1	2.35±0.11 a	37.84	10.81	1.45±0.09 ab	5.45
WFL	2.05±0.12 a	22.08	6.49	1.38±0.07 a	1.82

Light treatment	Green Bear			Big Chilli
Light treatment	FW (g)	DW (mg)	Height (mm)	FW (g)	DW (mg)	Height (mm)
RED(630)	0.94±0.05 bcde	49.56±2.81 abc	38.16±1.31 ef	0.74±0.04 bc	41.28±5.24 ab	42.77±1.11 f
R(630)8B2	0.90±0.05 bcde	49.58±2.81 abc	36.63±1.13 cde	0.81±0.05 bcd	42.01±2.45 ab	36.61±1.03 cd
R(630)7B3	0.84±0.05 abc	47.30±3.33 ab	33.15±1.00 ab	0.75±0.05 bc	42.82±2.59 abc	34.21±0.99 bc
R(630)5B5	0.79±0.05 ab	47.86±3.34 ab	34.59±0.88 bcd	0.74±0.04 bcd	41.89±2.19 ab	34.20±0.86 bc
R(630)3B7	0.71±0.05 a	43.16±2.70 a	32.94±0.77 ab	0.73±0.04 bc	46.04±2.37 bc	28.98±1.09 a
R(630)2B8	0.80±0.06 ab	45.01±3.61 a	30.94±0.78 a	0.76±0.05 bcd	43.65±2.53 bc	32.53±0.85 b
BLUE	0.79±0.05 ab	53.30±3.35 abcd	39.68±0.89 f	0.74±0.04 bc	41.88±2.46 ab	37.79±1.01 de
RED(660)	0.83±0.05 abc	47.80±2.49 ab	37.41±0.90 def	0.89±0.05 d	53.17±7.59 c	42.78±1.26 f
R(660)7B3	1.04±0.05 e	56.86±1.77 bcde	37.18±0.92 def	0.68±0.04 b	40.16±2.74 ab	32.92±1.13 b
R(660)5B5	0.95±0.05 bcde	58.26±3.12 cde	32.93±0.76 ab	0.83±0.05 cd	42.41±2.14 ab	34.37±1.14 bc
CW5R(630)5	0.99±0.05 cde	56.80±3.06 bcde	34.94±0.96 bcd	0.75±0.04 bcd	47.30±2.94 bc	38.83±1.14 de
Warm W	1.04±0.06 e	60.25±3.61 de	36.37±1.08 cde	0.81±0.05 bc	41.76±2.27 ab	40.70±1.19 ef
R(630)6B3FR1	0.88±0.05 abcde	50.44±2.82 abcd	34.96±0.98 bcd	0.70±0.04 bc	43.67±2.11 bc	32.41±0.87 b
R(630)4B4FR2	1.04±0.08 de	63.37±4.68 e	34.47±1.22 bcd	0.81±0.03 bcd	49.82±2.56 bc	33.02±1.16 b
R(630)3B6FR1	0.86±0.04 abcd	51.89±2.46 abcd	34.69±0.91 bcd	0.69±0.04 bc	42.69±1.96 abc	31.55±0.98 ab
WFL	0.87±0.06 abcde	50.63±3.17 abcd	33.74±0.83 abc	0.53±0.03 a	32.59±1.60 a	31.32±0.98 ab

不同光质的LED对蝴蝶兰组织培养增殖及生根的影响

Effect of LED in Different Light Qualities on Growth of Phalaenopsis Plantlets

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Light treatment	Green Bear			Big Chilli
Light treatment	Root length (mm)	Number of root	Root activity (mg·g^-1·h^-1)	Root length (mm)	Number of root	Root activity (mg·g^-1·h^-1)
RED(630)	19.11±0.76 a	3.78±0.22 cde	92.08±12.84 a	12.50±0.89 a	2.67±0.16 a	118.74±15.12 a
R(630)8B2	20.34±0.99 ab	3.36±0.20 abcd	106.1±16.47 ab	25.33±1.39 b	2.68±0.11 a	177.04±22.40 abc
R(630)7B3	20.50±0.86 ab	3.92±0.18 de	115.53±10.06 abc	30.65±1.42 cd	3.02±0.14 abc	185.91±20.58 abc
R(630)5B5	21.77±0.94 abc	2.88±0.16 a	148.8±26.45 abcd	37.38±1.88 fg	3.30±0.12 cde	201.35±30.95 abc
R(630)3B7	23.65±0.91 bcde	4.03±0.19 e	180.87±17.96 cd	36.20±1.38 efg	3.43±0.12 def	201.76±38.83 abc
R(630)2B8	21.08±1.02 abc	3.73±0.23 cde	211.99±18.74 de	36.56±1.75 efg	3.25±0.14 cde	271.84±49.93 cde
BLUE	22.80±1.18 bcd	3.07±0.20 ab	253.49±11.79 e	36.50±1.73 efg	3.42±0.14 def	323.20±73.13 de
RED(660)	20.93±0.97 ab	3.76±0.19 cde	166.72±3.95 bcd	33.77±1.51 def	3.82±0.14 f	148.01±16.17 ab
R(660)7B3	23.69±1.27 bcde	3.25±0.18 abc	182.35±25.72 cd	27.40±1.46 bc	3.19±0.14 cd	183.29±12.02 abc
R(660)5B5	20.82±0.95 ab	3.71±0.22 cde	259.68±29.63 e	31.12±2.77 cde	3.09±0.20 bc	261.83±39.75 bcde
CW5R(630)5	20.74±0.97 ab	3.53±0.16 bcde	171.98±33.48 bcd	31.54±1.90 cde	3.46±0.15 def	188.82±20.36 abc
Warm W	22.29±0.92 abcd	3.73±0.21 cde	211.05±17.48 de	30.66±2.00 cd	3.57±0.14 def	225.28±40.13 abcd
R(630)6B3FR1	24.27±1.01 cde	3.86±0.19 cde	260.63±29.06 e	39.22±1.83 fg	3.67±0.11 ef	351.65±24.40 ef
R(630)4B4FR2	26.40±1.29 e	3.75±0.15 cde	349.41±31.61 f	39.88±2.17 g	3.56±0.15 def	366.20±29.03 ef
R(630)3B6FR1	25.04±0.96 de	3.74±0.19 cde	369.49±36.35 f	40.35±2.08 g	3.58±0.15 def	449.72±57.66 f
WFL	20.53±1.06 ab	3.49±0.18 bcde	172.52±11.89 bcd	24.58±1.61 b	2.64±0.13 a	171.67±23.38 abc

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