植物学报 ›› 2016, Vol. 51 ›› Issue (1): 81-88.DOI: 10.11983/CBB14196
收稿日期:
2014-11-15
接受日期:
2015-04-14
出版日期:
2016-01-01
发布日期:
2016-02-01
通讯作者:
朱根发
作者简介:
? 共同第一作者
基金资助:
Guiping Ren1,2, Xiaojing Wang1, Genfa Zhu2*
Received:
2014-11-15
Accepted:
2015-04-14
Online:
2016-01-01
Published:
2016-02-01
Contact:
Zhu Genfa
About author:
? These authors contributed equally to this paper
摘要:
以蝴蝶兰(Phalaenopsis ssp.)栽培种绿熊(Green Bear)和大辣椒(Big Chilli)为培养材料, 研究不同光质的发光二极管(LED)组合对组织培养过程中增殖及生根的影响。结果表明, 红光更有利于蝴蝶兰单芽增殖、干重、鲜重以及株高的增加, 但不利于叶片叶绿素的积累; 蓝光有利于叶片叶绿素的积累, 并能提高根系活力; 远红光则对根长和根系活力的增加作用更显著。增殖扩繁阶段的最适LED为暖白, 2种蝴蝶兰芽增殖系数分别比白色荧光灯(对照)高出53.17%和46.37%。生根诱导阶段的最适LED组合为红:蓝:远红=3:6:1, 根长及根系活力均较对照显著增加。该研究结果为LED光源在蝴蝶兰组织培养中的应用奠定了基础。
任桂萍, 王小菁, 朱根发. 不同光质的LED对蝴蝶兰组织培养增殖及生根的影响. 植物学报, 2016, 51(1): 81-88.
Guiping Ren, Xiaojing Wang, Genfa Zhu. Effect of LED in Different Light Qualities on Growth of Phalaenopsis Plantlets. Chinese Bulletin of Botany, 2016, 51(1): 81-88.
Light treatment | Factors | Wavelength |
---|---|---|
RED(630) | Red | 630 nm |
R(630)8B2 | Red:Blue=8:2 | 630 nm/450-480 nm |
R(630)7B3 | Red:Blue=7:3 | 630 nm/450-480 nm |
R(630)5B5 | Red:Blue=5:5 | 630 nm/450-480 nm |
R(630)3B7 | Red:Blue=3:7 | 630 nm/450-480 nm |
R(630)2B8 | Red:Blue=2:8 | 630 nm/450-480 nm |
BLUE | Blue | 450-480 nm |
RED(660) | Red | 660 nm |
R(660)7B3 | Red:Blue=7:3 | 660 nm/450-480 nm |
R(660)5B5 | Red:Blue=5:5 | 660 nm/450-480 nm |
CW5R(630)5 | Cold white:Red=5:5 | 630 nm/400-780 nm |
Warm W | Warm white LED | 400 nm-780 nm (the range of 500-780 nm accounts for 91.80%) |
R(630)6B3FR1 | Red:Blue:Far red=6:3:1 | 630 nm/450-480 nm/730 nm |
R(630)4B4FR2 | Red:Blue:Far red=4:4:2 | 630 nm/450-480 nm/730 nm |
R(630)3B6FR1 | Red:Blue:Far red=3:6:1 | 630 nm/450-480 nm/730 nm |
WFL | White fluorescent tube | 360-750 nm |
表1 LED不同光质配比
Table 1 The ratio of different light qualities of LED
Light treatment | Factors | Wavelength |
---|---|---|
RED(630) | Red | 630 nm |
R(630)8B2 | Red:Blue=8:2 | 630 nm/450-480 nm |
R(630)7B3 | Red:Blue=7:3 | 630 nm/450-480 nm |
R(630)5B5 | Red:Blue=5:5 | 630 nm/450-480 nm |
R(630)3B7 | Red:Blue=3:7 | 630 nm/450-480 nm |
R(630)2B8 | Red:Blue=2:8 | 630 nm/450-480 nm |
BLUE | Blue | 450-480 nm |
RED(660) | Red | 660 nm |
R(660)7B3 | Red:Blue=7:3 | 660 nm/450-480 nm |
R(660)5B5 | Red:Blue=5:5 | 660 nm/450-480 nm |
CW5R(630)5 | Cold white:Red=5:5 | 630 nm/400-780 nm |
Warm W | Warm white LED | 400 nm-780 nm (the range of 500-780 nm accounts for 91.80%) |
R(630)6B3FR1 | Red:Blue:Far red=6:3:1 | 630 nm/450-480 nm/730 nm |
R(630)4B4FR2 | Red:Blue:Far red=4:4:2 | 630 nm/450-480 nm/730 nm |
R(630)3B6FR1 | Red:Blue:Far red=3:6:1 | 630 nm/450-480 nm/730 nm |
WFL | White fluorescent tube | 360-750 nm |
Light treatment | Green Bear | Big Chilli | ||||
---|---|---|---|---|---|---|
Multiplication coefficient | Ratio (%) (number of shoot≥3) | Ratio (%) (number of shoot≥4) | Multiplication coefficient | Ratio (%) (number of shoot≥3) | ||
RED(630) | 2.85±0.16 c | 51.39 | 25.00 | 2.20±0.14 g | 34.55 | |
R(630)8B2 | 2.26±0.13 a | 38.46 | 11.54 | 1.85±0.11 cdef | 18.52 | |
R(630)7B3 | 2.33±0.14 a | 36.84 | 13.16 | 1.78±0.12 bcdef | 12.73 | |
R(630)5B5 | 2.19±0.15 a | 32.43 | 9.46 | 1.63±0.09 abcde | 9.26 | |
R(630)3B7 | 2.14±0.12 a | 25.71 | 10.00 | 1.69±0.11 abcdef | 14.55 | |
R(630)2B8 | 2.37±0.11 a | 36.90 | 11.90 | 1.50±0.08 abc | 3.57 | |
BLUE | 2.27±0.13 a | 33.33 | 14.67 | 1.66±0.13 abcdef | 16.00 | |
RED(660) | 2.80±0.15 bc | 56.25 | 25.00 | 2.00±0.12 fg | 32.14 | |
R(660)7B3 | 2.13±0.13 a | 35.94 | 9.38 | 1.86±0.10 defg | 21.57 | |
R(660)5B5 | 2.39±0.14 ab | 40.26 | 18.18 | 1.75±0.11 bcdef | 19.23 | |
CW5R(630)5 | 2.43±0.15 ab | 38.67 | 20.00 | 1.94±0.12 efg | 20.75 | |
Warm W | 3.14±0.17 c | 62.16 | 36.49 | 2.02±0.12 fg | 26.98 | |
R(630)6B3FR1 | 2.32±0.17 a | 34.78 | 14.49 | 1.49±0.09 ab | 6.38 | |
R(630)4B4FR2 | 2.29±0.19 a | 31.82 | 13.64 | 1.56±0.09 abcd | 8.33 | |
R(630)3B6FR1 | 2.35±0.11 a | 37.84 | 10.81 | 1.45±0.09 ab | 5.45 | |
WFL | 2.05±0.12 a | 22.08 | 6.49 | 1.38±0.07 a | 1.82 |
表2 不同光质的LED处理下蝴蝶兰单芽增殖系数
Table 2 Multiplication coefficient of Phalaenopsis shoot under different light qualities of LED
Light treatment | Green Bear | Big Chilli | ||||
---|---|---|---|---|---|---|
Multiplication coefficient | Ratio (%) (number of shoot≥3) | Ratio (%) (number of shoot≥4) | Multiplication coefficient | Ratio (%) (number of shoot≥3) | ||
RED(630) | 2.85±0.16 c | 51.39 | 25.00 | 2.20±0.14 g | 34.55 | |
R(630)8B2 | 2.26±0.13 a | 38.46 | 11.54 | 1.85±0.11 cdef | 18.52 | |
R(630)7B3 | 2.33±0.14 a | 36.84 | 13.16 | 1.78±0.12 bcdef | 12.73 | |
R(630)5B5 | 2.19±0.15 a | 32.43 | 9.46 | 1.63±0.09 abcde | 9.26 | |
R(630)3B7 | 2.14±0.12 a | 25.71 | 10.00 | 1.69±0.11 abcdef | 14.55 | |
R(630)2B8 | 2.37±0.11 a | 36.90 | 11.90 | 1.50±0.08 abc | 3.57 | |
BLUE | 2.27±0.13 a | 33.33 | 14.67 | 1.66±0.13 abcdef | 16.00 | |
RED(660) | 2.80±0.15 bc | 56.25 | 25.00 | 2.00±0.12 fg | 32.14 | |
R(660)7B3 | 2.13±0.13 a | 35.94 | 9.38 | 1.86±0.10 defg | 21.57 | |
R(660)5B5 | 2.39±0.14 ab | 40.26 | 18.18 | 1.75±0.11 bcdef | 19.23 | |
CW5R(630)5 | 2.43±0.15 ab | 38.67 | 20.00 | 1.94±0.12 efg | 20.75 | |
Warm W | 3.14±0.17 c | 62.16 | 36.49 | 2.02±0.12 fg | 26.98 | |
R(630)6B3FR1 | 2.32±0.17 a | 34.78 | 14.49 | 1.49±0.09 ab | 6.38 | |
R(630)4B4FR2 | 2.29±0.19 a | 31.82 | 13.64 | 1.56±0.09 abcd | 8.33 | |
R(630)3B6FR1 | 2.35±0.11 a | 37.84 | 10.81 | 1.45±0.09 ab | 5.45 | |
WFL | 2.05±0.12 a | 22.08 | 6.49 | 1.38±0.07 a | 1.82 |
Light treatment | Green Bear | Big Chilli | |||||
---|---|---|---|---|---|---|---|
FW (g) | DW (mg) | Height (mm) | FW (g) | DW (mg) | Height (mm) | ||
RED(630) | 0.94±0.05 bcde | 49.56±2.81 abc | 38.16±1.31 ef | 0.74±0.04 bc | 41.28±5.24 ab | 42.77±1.11 f | |
R(630)8B2 | 0.90±0.05 bcde | 49.58±2.81 abc | 36.63±1.13 cde | 0.81±0.05 bcd | 42.01±2.45 ab | 36.61±1.03 cd | |
R(630)7B3 | 0.84±0.05 abc | 47.30±3.33 ab | 33.15±1.00 ab | 0.75±0.05 bc | 42.82±2.59 abc | 34.21±0.99 bc | |
R(630)5B5 | 0.79±0.05 ab | 47.86±3.34 ab | 34.59±0.88 bcd | 0.74±0.04 bcd | 41.89±2.19 ab | 34.20±0.86 bc | |
R(630)3B7 | 0.71±0.05 a | 43.16±2.70 a | 32.94±0.77 ab | 0.73±0.04 bc | 46.04±2.37 bc | 28.98±1.09 a | |
R(630)2B8 | 0.80±0.06 ab | 45.01±3.61 a | 30.94±0.78 a | 0.76±0.05 bcd | 43.65±2.53 bc | 32.53±0.85 b | |
BLUE | 0.79±0.05 ab | 53.30±3.35 abcd | 39.68±0.89 f | 0.74±0.04 bc | 41.88±2.46 ab | 37.79±1.01 de | |
RED(660) | 0.83±0.05 abc | 47.80±2.49 ab | 37.41±0.90 def | 0.89±0.05 d | 53.17±7.59 c | 42.78±1.26 f | |
R(660)7B3 | 1.04±0.05 e | 56.86±1.77 bcde | 37.18±0.92 def | 0.68±0.04 b | 40.16±2.74 ab | 32.92±1.13 b | |
R(660)5B5 | 0.95±0.05 bcde | 58.26±3.12 cde | 32.93±0.76 ab | 0.83±0.05 cd | 42.41±2.14 ab | 34.37±1.14 bc | |
CW5R(630)5 | 0.99±0.05 cde | 56.80±3.06 bcde | 34.94±0.96 bcd | 0.75±0.04 bcd | 47.30±2.94 bc | 38.83±1.14 de | |
Warm W | 1.04±0.06 e | 60.25±3.61 de | 36.37±1.08 cde | 0.81±0.05 bc | 41.76±2.27 ab | 40.70±1.19 ef | |
R(630)6B3FR1 | 0.88±0.05 abcde | 50.44±2.82 abcd | 34.96±0.98 bcd | 0.70±0.04 bc | 43.67±2.11 bc | 32.41±0.87 b | |
R(630)4B4FR2 | 1.04±0.08 de | 63.37±4.68 e | 34.47±1.22 bcd | 0.81±0.03 bcd | 49.82±2.56 bc | 33.02±1.16 b | |
R(630)3B6FR1 | 0.86±0.04 abcd | 51.89±2.46 abcd | 34.69±0.91 bcd | 0.69±0.04 bc | 42.69±1.96 abc | 31.55±0.98 ab | |
WFL | 0.87±0.06 abcde | 50.63±3.17 abcd | 33.74±0.83 abc | 0.53±0.03 a | 32.59±1.60 a | 31.32±0.98 ab |
表3 不同光质的LED对蝴蝶兰增殖培养中形态指标的影响
Table 3 Effects of different light qualities of LED on morphology of Phalaenopsis
Light treatment | Green Bear | Big Chilli | |||||
---|---|---|---|---|---|---|---|
FW (g) | DW (mg) | Height (mm) | FW (g) | DW (mg) | Height (mm) | ||
RED(630) | 0.94±0.05 bcde | 49.56±2.81 abc | 38.16±1.31 ef | 0.74±0.04 bc | 41.28±5.24 ab | 42.77±1.11 f | |
R(630)8B2 | 0.90±0.05 bcde | 49.58±2.81 abc | 36.63±1.13 cde | 0.81±0.05 bcd | 42.01±2.45 ab | 36.61±1.03 cd | |
R(630)7B3 | 0.84±0.05 abc | 47.30±3.33 ab | 33.15±1.00 ab | 0.75±0.05 bc | 42.82±2.59 abc | 34.21±0.99 bc | |
R(630)5B5 | 0.79±0.05 ab | 47.86±3.34 ab | 34.59±0.88 bcd | 0.74±0.04 bcd | 41.89±2.19 ab | 34.20±0.86 bc | |
R(630)3B7 | 0.71±0.05 a | 43.16±2.70 a | 32.94±0.77 ab | 0.73±0.04 bc | 46.04±2.37 bc | 28.98±1.09 a | |
R(630)2B8 | 0.80±0.06 ab | 45.01±3.61 a | 30.94±0.78 a | 0.76±0.05 bcd | 43.65±2.53 bc | 32.53±0.85 b | |
BLUE | 0.79±0.05 ab | 53.30±3.35 abcd | 39.68±0.89 f | 0.74±0.04 bc | 41.88±2.46 ab | 37.79±1.01 de | |
RED(660) | 0.83±0.05 abc | 47.80±2.49 ab | 37.41±0.90 def | 0.89±0.05 d | 53.17±7.59 c | 42.78±1.26 f | |
R(660)7B3 | 1.04±0.05 e | 56.86±1.77 bcde | 37.18±0.92 def | 0.68±0.04 b | 40.16±2.74 ab | 32.92±1.13 b | |
R(660)5B5 | 0.95±0.05 bcde | 58.26±3.12 cde | 32.93±0.76 ab | 0.83±0.05 cd | 42.41±2.14 ab | 34.37±1.14 bc | |
CW5R(630)5 | 0.99±0.05 cde | 56.80±3.06 bcde | 34.94±0.96 bcd | 0.75±0.04 bcd | 47.30±2.94 bc | 38.83±1.14 de | |
Warm W | 1.04±0.06 e | 60.25±3.61 de | 36.37±1.08 cde | 0.81±0.05 bc | 41.76±2.27 ab | 40.70±1.19 ef | |
R(630)6B3FR1 | 0.88±0.05 abcde | 50.44±2.82 abcd | 34.96±0.98 bcd | 0.70±0.04 bc | 43.67±2.11 bc | 32.41±0.87 b | |
R(630)4B4FR2 | 1.04±0.08 de | 63.37±4.68 e | 34.47±1.22 bcd | 0.81±0.03 bcd | 49.82±2.56 bc | 33.02±1.16 b | |
R(630)3B6FR1 | 0.86±0.04 abcd | 51.89±2.46 abcd | 34.69±0.91 bcd | 0.69±0.04 bc | 42.69±1.96 abc | 31.55±0.98 ab | |
WFL | 0.87±0.06 abcde | 50.63±3.17 abcd | 33.74±0.83 abc | 0.53±0.03 a | 32.59±1.60 a | 31.32±0.98 ab |
图1 不同光质LED对蝴蝶兰叶片叶绿素含量的影响 (A) 绿熊叶绿素含量; (B) 大辣椒叶绿素含量。WFL: 白色荧光灯
Figure 1 Effects of different light qualities of LED on chlorophyll content of Phalaenopsis leaves (A) Chlorophyll content of Green Bear (B) Chlorophyll content of Big Chilli. WFL: white fluorescent tube
图2 部分LED光质下蝴蝶兰丛生芽增殖及幼苗生长状况 (A)-(D) 增殖培养60天后绿熊的丛生芽; (E)-(H) 增殖培养60天后大辣椒的丛生芽; (I)-(L) 生根培养60天后绿熊的幼苗; (M)-(P) 生根培养60天后大辣椒的幼苗。Bar=2 cm
Figure 2 The growth conditions of partial Phalaenopsis’s plantlet under different light quality of LED(A)-(D) Multiple shoots of Green Bear after 60 day’s multiplication culture; (E)-(H) Multiple shoots of Big Chilli after 60 day’s multiplication culture; (I)-(L) Plantlet of Green Bear after 60 day’s rooting culture; (M)-(P) Plantlet of Big Chilli after 60 day’s rooting culture. Bar=2 cm
Light treatment | Green Bear | Big Chilli | ||||||
---|---|---|---|---|---|---|---|---|
Root length (mm) | Number of root | Root activity (mg·g-1·h-1) | Root length (mm) | Number of root | Root activity (mg·g-1·h-1) | |||
RED(630) | 19.11±0.76 a | 3.78±0.22 cde | 92.08±12.84 a | 12.50±0.89 a | 2.67±0.16 a | 118.74±15.12 a | ||
R(630)8B2 | 20.34±0.99 ab | 3.36±0.20 abcd | 106.1±16.47 ab | 25.33±1.39 b | 2.68±0.11 a | 177.04±22.40 abc | ||
R(630)7B3 | 20.50±0.86 ab | 3.92±0.18 de | 115.53±10.06 abc | 30.65±1.42 cd | 3.02±0.14 abc | 185.91±20.58 abc | ||
R(630)5B5 | 21.77±0.94 abc | 2.88±0.16 a | 148.8±26.45 abcd | 37.38±1.88 fg | 3.30±0.12 cde | 201.35±30.95 abc | ||
R(630)3B7 | 23.65±0.91 bcde | 4.03±0.19 e | 180.87±17.96 cd | 36.20±1.38 efg | 3.43±0.12 def | 201.76±38.83 abc | ||
R(630)2B8 | 21.08±1.02 abc | 3.73±0.23 cde | 211.99±18.74 de | 36.56±1.75 efg | 3.25±0.14 cde | 271.84±49.93 cde | ||
BLUE | 22.80±1.18 bcd | 3.07±0.20 ab | 253.49±11.79 e | 36.50±1.73 efg | 3.42±0.14 def | 323.20±73.13 de | ||
RED(660) | 20.93±0.97 ab | 3.76±0.19 cde | 166.72±3.95 bcd | 33.77±1.51 def | 3.82±0.14 f | 148.01±16.17 ab | ||
R(660)7B3 | 23.69±1.27 bcde | 3.25±0.18 abc | 182.35±25.72 cd | 27.40±1.46 bc | 3.19±0.14 cd | 183.29±12.02 abc | ||
R(660)5B5 | 20.82±0.95 ab | 3.71±0.22 cde | 259.68±29.63 e | 31.12±2.77 cde | 3.09±0.20 bc | 261.83±39.75 bcde | ||
CW5R(630)5 | 20.74±0.97 ab | 3.53±0.16 bcde | 171.98±33.48 bcd | 31.54±1.90 cde | 3.46±0.15 def | 188.82±20.36 abc | ||
Warm W | 22.29±0.92 abcd | 3.73±0.21 cde | 211.05±17.48 de | 30.66±2.00 cd | 3.57±0.14 def | 225.28±40.13 abcd | ||
R(630)6B3FR1 | 24.27±1.01 cde | 3.86±0.19 cde | 260.63±29.06 e | 39.22±1.83 fg | 3.67±0.11 ef | 351.65±24.40 ef | ||
R(630)4B4FR2 | 26.40±1.29 e | 3.75±0.15 cde | 349.41±31.61 f | 39.88±2.17 g | 3.56±0.15 def | 366.20±29.03 ef | ||
R(630)3B6FR1 | 25.04±0.96 de | 3.74±0.19 cde | 369.49±36.35 f | 40.35±2.08 g | 3.58±0.15 def | 449.72±57.66 f | ||
WFL | 20.53±1.06 ab | 3.49±0.18 bcde | 172.52±11.89 bcd | 24.58±1.61 b | 2.64±0.13 a | 171.67±23.38 abc |
表4 不同光质的LED对蝴蝶兰组培苗生根的影响
Table 4 Effects of different light qualities of LED on rooting of Phalaenopsis plantlet
Light treatment | Green Bear | Big Chilli | ||||||
---|---|---|---|---|---|---|---|---|
Root length (mm) | Number of root | Root activity (mg·g-1·h-1) | Root length (mm) | Number of root | Root activity (mg·g-1·h-1) | |||
RED(630) | 19.11±0.76 a | 3.78±0.22 cde | 92.08±12.84 a | 12.50±0.89 a | 2.67±0.16 a | 118.74±15.12 a | ||
R(630)8B2 | 20.34±0.99 ab | 3.36±0.20 abcd | 106.1±16.47 ab | 25.33±1.39 b | 2.68±0.11 a | 177.04±22.40 abc | ||
R(630)7B3 | 20.50±0.86 ab | 3.92±0.18 de | 115.53±10.06 abc | 30.65±1.42 cd | 3.02±0.14 abc | 185.91±20.58 abc | ||
R(630)5B5 | 21.77±0.94 abc | 2.88±0.16 a | 148.8±26.45 abcd | 37.38±1.88 fg | 3.30±0.12 cde | 201.35±30.95 abc | ||
R(630)3B7 | 23.65±0.91 bcde | 4.03±0.19 e | 180.87±17.96 cd | 36.20±1.38 efg | 3.43±0.12 def | 201.76±38.83 abc | ||
R(630)2B8 | 21.08±1.02 abc | 3.73±0.23 cde | 211.99±18.74 de | 36.56±1.75 efg | 3.25±0.14 cde | 271.84±49.93 cde | ||
BLUE | 22.80±1.18 bcd | 3.07±0.20 ab | 253.49±11.79 e | 36.50±1.73 efg | 3.42±0.14 def | 323.20±73.13 de | ||
RED(660) | 20.93±0.97 ab | 3.76±0.19 cde | 166.72±3.95 bcd | 33.77±1.51 def | 3.82±0.14 f | 148.01±16.17 ab | ||
R(660)7B3 | 23.69±1.27 bcde | 3.25±0.18 abc | 182.35±25.72 cd | 27.40±1.46 bc | 3.19±0.14 cd | 183.29±12.02 abc | ||
R(660)5B5 | 20.82±0.95 ab | 3.71±0.22 cde | 259.68±29.63 e | 31.12±2.77 cde | 3.09±0.20 bc | 261.83±39.75 bcde | ||
CW5R(630)5 | 20.74±0.97 ab | 3.53±0.16 bcde | 171.98±33.48 bcd | 31.54±1.90 cde | 3.46±0.15 def | 188.82±20.36 abc | ||
Warm W | 22.29±0.92 abcd | 3.73±0.21 cde | 211.05±17.48 de | 30.66±2.00 cd | 3.57±0.14 def | 225.28±40.13 abcd | ||
R(630)6B3FR1 | 24.27±1.01 cde | 3.86±0.19 cde | 260.63±29.06 e | 39.22±1.83 fg | 3.67±0.11 ef | 351.65±24.40 ef | ||
R(630)4B4FR2 | 26.40±1.29 e | 3.75±0.15 cde | 349.41±31.61 f | 39.88±2.17 g | 3.56±0.15 def | 366.20±29.03 ef | ||
R(630)3B6FR1 | 25.04±0.96 de | 3.74±0.19 cde | 369.49±36.35 f | 40.35±2.08 g | 3.58±0.15 def | 449.72±57.66 f | ||
WFL | 20.53±1.06 ab | 3.49±0.18 bcde | 172.52±11.89 bcd | 24.58±1.61 b | 2.64±0.13 a | 171.67±23.38 abc |
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