植物学报 ›› 2017, Vol. 52 ›› Issue (6): 733-742.DOI: 10.11983/CBB16218

• 研究报告 • 上一篇    下一篇

葡萄种间杂交砧木育种F1代植株耐盐性分析

付晴晴, 孙鲁龙, 翟衡, 杜远鹏*()   

  1. 山东农业大学园艺科学与工程学院, 作物生物学国家重点实验室, 泰安 271018
  • 收稿日期:2016-11-14 接受日期:2016-12-11 出版日期:2017-11-01 发布日期:2018-02-22
  • 通讯作者: 杜远鹏
  • 基金资助:
    国家现代葡萄产业技术体系建设专项(No.CARS-30)与长江学者和创新团队发展计划(No.IRT15R42)

Salt Tolerant Evaluation of F1-generation Hybrids in Grape

Fu Qingqing, Sun Lulong, Zhai Heng, Du Yuanpeng*()   

  1. Key Laboratory of Crop Biology of Shandong Province/College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
  • Received:2016-11-14 Accepted:2016-12-11 Online:2017-11-01 Published:2018-02-22
  • Contact: Du Yuanpeng

摘要:

以耐盐性较强的砧木1103P为对照品种, 对左山一×SO4杂种砧木F1代的6个株系(A15、A17、A34、A35、A38和A48)及左山一×101-1杂种F1代2个株系(B24和B26)的一年生盆栽扦插苗进行100 mmol·L-1 NaCl胁迫处理, 以各自无盐胁迫为对照处理。20天后, 根据表型计算盐害指数, 测定叶绿素含量、光合气体交换参数、叶绿素荧光参数以及生长量指标; 以各项生长指标的耐盐系数为耐盐指标, 通过主成分分析、相关性分析、隶属函数分析和聚类分析等方法对葡萄株系进行综合评价。结果表明, A34和A35植株无盐害症状, 盐害级数为0; A15和A17植株有少部分叶片边缘焦枯, 盐害级数为1。盐胁迫大幅度降低了1103P和B26等株系的叶绿素含量、光合速率、新梢生长量和生物量; 而A15、A17、A34和A35植株的各项指标降低幅度较小。将生物量等12个单项指标转换成3个相互独立的综合指标, 通过聚类分析, 发现A34、A35、A15和A17植株的耐盐性较强, A38、A48和B24植株的耐盐性中等, 1103P和B26植株的耐盐性较弱, 与盐害分级结果一致。

关键词: 葡萄砧木, 杂交株系, 耐盐性, 鉴定

Abstract:

With rootstock 1103P used as a negative control, annual cuttings of 6 hybrids of Zuoshan1 × SO4 and 2 hybrids of Zuoshan1 × 101-1 were used as materials with 100 mmol·L-1 NaCl irrigation; the chlorophyll content and photosynthetic gas exchange, chlorophyll fluorescence and growth values were measured after 20 days of NaCl treatment (with non-salt treatment as the negative control). The salt tolerance index was evaluated by each growth index; comprehensive assessment was based on principal-component, correlation, membership-function and cluster analyses: A34 and A35 plants showing no salt damage were classified as class 0; A15 and A17 plants showing at a small part of withering at plant leaf margins were classified as class I. Salt stress reduced the chlorophyll content, photosynthetic rate, new shoot growth and biomass in 1103P and B26 but A15, A17, A34 and A35 plants showed less reduction. The biomass and 12 indexes were converted to 3 independent comprehensive indexes. According to cluster analysis, A34, A35, A15 and A17 had relatively higher salt tolerance; A38, A48 and B24 had moderate salt resistance; and 1103P and B26 had more salt sensitivity. These results were consistent with the salt damage classification.

Key words: grape rootstocks, hybrid strains, salt tolerance, evaluation