植物学报 ›› 2023, Vol. 58 ›› Issue (5): 831-842.DOI: 10.11983/CBB22194
• 专题论坛 • 上一篇
王露露1,2,3, 杨智1,2,3, 杨永1,2,3,*()
收稿日期:
2022-08-12
接受日期:
2023-02-09
出版日期:
2023-09-01
发布日期:
2023-09-21
通讯作者:
*E-mail: yangyong@njfu.edu.cn
基金资助:
Wang Lulu1,2,3, Yang Zhi1,2,3, Yang Yong1,2,3,*()
Received:
2022-08-12
Accepted:
2023-02-09
Online:
2023-09-01
Published:
2023-09-21
Contact:
*E-mail: yangyong@njfu.edu.cn
摘要: 物种的准确鉴定是生物资源利用和物种保护的重要基础。传统的物种鉴定依赖于分类学专业人员, 而培养分类学专业人员需要耗费大量时间, 是分类学知识利用的瓶颈。利用多通道电子检索表和智能识别也因个体发育阶段的不同和形态变异而难以准确鉴定物种。DNA条形码是基于DNA序列的鉴定技术, 不受个体发育和形态多态性的影响, 为物种快速鉴定提供了有效的解决方案。该文综述了DNA条形码的类型和发展趋势。DNA条形码的发展历史可分为2个阶段: (1) 以一代测序为序列获取方式的单片段或少量片段时期; (2) 以新一代高通量测序为序列获取方式的超级DNA条形码时期。该文比较了2个发展阶段DNA条形码的主要特点、研究方法和物种鉴定能力, 认为基于质体基因组的超级DNA条形码物种鉴定能力更强, 发展超级DNA条形码将成为植物条形码研究的主要趋势。同时进一步总结了超级DNA条形码发展所面临的困境和解决思路, 指出研究材料仍是当前限制超级DNA条形码发展的关键因素, 提出利用标本组学方法捕获大量历史馆藏标本的基因组序列, 可为开展超级DNA条形码研究提供省时、高效和经济的解决方案。通过挖掘植物基因组数据获得单拷贝和低拷贝核基因并应用于物种条形码研究, 不仅能提升分辨率, 而且可解决杂交和不完全谱系分选等复杂进化历史类群的分类问题。建议利用标本馆保存的珍贵标本资源建立植物超级DNA条形码参考序列数据库。
王露露, 杨智, 杨永. 利用标本组学推进植物超级DNA条形码研究. 植物学报, 2023, 58(5): 831-842.
Wang Lulu, Yang Zhi, Yang Yong. Plant Ultra-barcoding Using Herbariomics. Chinese Bulletin of Botany, 2023, 58(5): 831-842.
图1 Web of Science数据库历年发表的DNA条形码论文统计 (A) 2003-2022年DNA条形码相关文章数量; (B) 不同学科研究方向DNA条形码相关文章数量
Figure 1 Publications of DNA barcodes in the Web of Science database over years (A) Number of articles related to DNA barcoding published in 2003-2022; (B) Number of articles related to DNA barcoding published in different disciplines
测序技术 | 实验成本 | 最终成本 | DNA质量要求 | 同源序列的识别能力 | 生物信息学专门知识 | 是否适用于标本材料 |
---|---|---|---|---|---|---|
简化基因组测序 | 高 | 低 | 中-高 | 难 | 中级/高级 | 否 |
转录组测序 | 低 | 低 | 中-高 | 相对容易 | 中级 | 否 |
杂交捕获测序 | 低 | 中 | 低-中 | 容易 | 中级/高级 | 是 |
扩增子测序 | 低 | 中 | 低-中 | 容易 | 初级 | 是 |
基因组浅层测序 | 低 | 中 | 低-中 | 容易 | 初级 | 是 |
表1 5种常用基因组划分方法的比较
Table 1 Comparison of five commonly used methods for genome partitioning
测序技术 | 实验成本 | 最终成本 | DNA质量要求 | 同源序列的识别能力 | 生物信息学专门知识 | 是否适用于标本材料 |
---|---|---|---|---|---|---|
简化基因组测序 | 高 | 低 | 中-高 | 难 | 中级/高级 | 否 |
转录组测序 | 低 | 低 | 中-高 | 相对容易 | 中级 | 否 |
杂交捕获测序 | 低 | 中 | 低-中 | 容易 | 中级/高级 | 是 |
扩增子测序 | 低 | 中 | 低-中 | 容易 | 初级 | 是 |
基因组浅层测序 | 低 | 中 | 低-中 | 容易 | 初级 | 是 |
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