植物学报 ›› 2023, Vol. 58 ›› Issue (1): 132-139.DOI: 10.11983/CBB22169

所属专题: 杂粮生物学专辑 (2023年58卷1期)

• 研究报告 • 上一篇    下一篇

豌豆茎基腐病病原菌分离鉴定及其对杀菌剂的敏感性测定

宋雅静1,2, 欧晋稳2,3, 张古文2, 冯志娟2, 卜远鹏2, 王斌2, 龚亚明2, 徐建强1,*(), 刘娜2,*()   

  1. 1河南科技大学园艺与植物保护学院, 洛阳 471003
    2浙江省农业科学院蔬菜研究所, 农产品质量安全危害因子与风险防控国家重点实验室, 农业农村部南方豆类蔬菜种质创新与分子育种重点实验室, 杭州 310021
    3浙江农林大学园艺科学学院, 杭州 311300
  • 收稿日期:2022-07-27 接受日期:2022-11-14 出版日期:2023-01-01 发布日期:2023-01-05
  • 通讯作者: *E-mail: xujqhust@126.com;ln200811@163.com
  • 基金资助:
    浙江省基础公益研究计划(LGN20C150006);浙江省“十四五”蔬菜新品种选育重大科技专项(2021C02065-6-2);国家自然科学基金(31872114)

Pathogen Identification of Pea Crown Rot and Its Sensitivity to Fungicides

Yajing Song1,2, Jinwen Ou2,3, Guwen Zhang2, Zhijuan Feng2, Yuanpeng Bu2, Bin Wang2, Yaming Gong2, Jianqiang Xu1,*(), Na Liu2,*()   

  1. 1College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471003, China
    2Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Ministry of Agriculture and Rural Affairs, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
    3College of Horticulture Science, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China

摘要: 豌豆(Pisum sativum)是我国重要的豆类经济作物, 病害对豌豆生产造成重大经济损失。通过形态学观察、分子鉴定以及致病性测定, 最终确定引起豌豆茎基腐病的3种病原菌分别为尖孢镰刀菌(Fusarium oxysporum)、芸苔链格孢菌(Alternaria brassicae)和格氏镰刀菌(F. grosmichelii), 优势菌株为尖孢镰刀菌, 分离率为53.6%。室内毒力测定结果表明, 5种供试杀菌剂对3种病原菌的菌丝生长均有抑制作用, 其中咯菌腈和戊唑醇的抑菌效果最好。研究结果为豌豆茎基腐病的防治提供了科学依据。

关键词: 豌豆, 茎基腐病, 分离与鉴定, 杀菌剂, 敏感性测定

Abstract: Pea (Pisum sativum) is one of the most important economic crops of beans in China. The occurrence of diseases will cause great economic losses in pea production. Through morphological observation, molecular identification and pathogenicity determination, the results showed that the three kinds of pathogenic fungi causing pea crown rot were Fusarium oxysporum, Alternaria brassicae and F. grosmichelii, among which F. oxysporum was the most pathogenic, with the isolation frequency of 53.6%. The results of indoor toxicity measurement showed that five fungicides had inhibitory effects on the mycelial growth of three kinds of pathogenic bacteria, among which, the inhibition effect of fludioxonil and tebuconazole have top results. The results provide a scientific basis for the prevention and control of pea crown rot.

Key words: pea, crown rot, isolation and identification, fungicides, sensitivity determination