Chin Bull Bot ›› 2019, Vol. 54 ›› Issue (4): 486-496.doi: 10.11983/CBB18139

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Expression Analysis of Key Genes in Gibberellin Biosynthesis and Related Phytohormonal Dynamics During Sugarcane Internode Elongation

Fan Yegeng1,Qiu Lihang1,Huang Xing1,Zhou Huiwen1,Gan Chongkun2,Li Yangrui1,Yang Rongzhong1,Wu Jianming1,*(),Chen Rongfa1,*()   

  1. 1 Key Laboratory of Sugarcane Biotechnology and Genetic Improvement, Ministry of Agriculture/Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Guangxi Zhuang Autonomous Region Academy of Agricultural Sciences, Nanning 530007, China
    2 Chongzuo Jiangzhou Soil Fertility Station, Chongzuo 532200, China
  • Received:2018-06-19 Accepted:2018-12-10 Online:2020-01-08 Published:2019-07-01
  • Contact: Wu Jianming,Chen Rongfa E-mail:wujianming2004@126.com;389001826@qq.com

Abstract:

In this study, Saccharum officinarum GT42 was used as the research material. Second internode tissue of sugarcane at the non-elongation stage (Ls1), early elongation stage (Ls2) and elongation stage (Ls3) was collected to measure plant hormones such as gibberellic acid (GA), indole acetic acid (IAA), brassinosteroids (BR), cytokinin (CTK), ethylene (ETH) and abscisic acid (ABA). The differential expression of the key genes GA20-Oxidase1, GID1 and GAI was analyzed by qRT-PCR. During the elongation stage, the contents of GA and IAA showed an upward trend, but CTK and ABA contents showed a downward trend. ETH content was increased at first and then decreased, whereas BR content did not change. The expression of GA20-Oxidase 1 and GID1 increased, and that of GAI decreased, which was closely related to the changes in GA content. Therefore, we considered that the internode elongation of sugarcane is mainly related to GA and IAA, then are CTK and ABA, and ETH is regulated by IAA to affect internode elongation. The interaction between these phytohormones regulates the expression of GA20-Oxidase 1, GID1 and GAI, which affects the content and signal transduction of GA, thereby affecting the internode elongation of sugarcane. The study clarifies the differential expression of key genes in the gibberellin biosynthesis pathway and signal transduction process, along with the dynamic changes of plant hormone content during internode elongation of sugarcane.

Key words: phytohormone, gibberellin, gene expression analysis, internode elongation, sugarcane

Table 1

Primers for real-time quantitative PCR"

Genes Sequences of primers (5'-3') Tm (°C) Product length (bp)
25S rRNA F: CGAATACGAACCGTGAAAGC 58.4 164
R: ACAATGATAGGAAGAGCCGACA 59.0
GA20-Oxidase1 F: CCGTCGTGGACTACTTCG 54.0 205
R: CACGGCGGGTAGTAGTTGAG 58.3
GID1 F: GGTCATCCTCTTCTTCCACGG 60.8 119
R: AGTTGACGGACACCACGACG 61.3
GAI F: GCGACGACAAGGATGAGGA 58.8 186
R: TGAAGCGGTCAAGGAATGTG 59.2

Table 2

The elongation of sugarcane at different stages (means±SD)"

Period Plant height (cm) Elongation rate (cm·d-1) Internode number Average internode length (cm)
Ls1 39.38±1.10 0.44±0.01 4±1 6.09±0.28
Ls2 68.37±1.25** 0.97±0.07** 7±1** 8.05±0.18**
Ls3 147.13±0.85** 2.63±0.06** 12±1** 11.43±0.07**

Figure 1

Changes of sugarcane elongation at different stages (A) The sugarcane plant height of non-elongation stage (Ls1); (B) The sugarcane plant height of early elongation stage (Ls2); (C) The sugarcane plant height of elongation stage (Ls3). Bars=10 cm"

Figure 2

Changes of endogenous hormone contents in sugarcane internode during different elongation stages (A) Indole acetic acid (IAA); (B) Brassinosteroids (BR); (C) Cytokinin (CTK); (D) Abscisic acid (ABA); (E) Ethylene (ETH). The different lowercase letters indicate significant differences (P<0.05), and the different capital letters indicate extremely significant differences (P<0.01). Ls1-Ls3 see Table 2."

Figure 3

The changes of gibberellin content and genes differential expression of synthetic pathways during sugarcane internode elongation (A) The content of gibberellic acid (GA); (B) The expression level of GA20-Oxidase1; (C) The expression level of GID1; (D) The expression level of GAI. The different lowercase letters indicate significant differences (P<0.05), and the different capital letters indicate extremely significant differences (P<0.01). Ls1-Ls3 see Table 2."

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