Whole-genome Analysis of CCT Gene Family and Their Responses to Phytohormones in Aegilops tauschii

doi:10.11983/CBB16054

Abstract

Abstract: The control of flowering time is a crucial environmental adaptation in plants; numerous CCT domain genes control flowering in plants. Bioinformatics was used for a genome-wide research of CCT domain genes in Aegilops tauschii. In this study, 26 CCT domain genes were identified in A. tauschii, distributed on seven chromosomes of the A. tauschii genome. The predicted molecular weight of this family was spread over 14.9 to 83.2 kDa, and 25 proteins contain a complete CCT conserved structure domain. Twelve pairs of A. tauschii-Triticum urartu and 9 pairs of A. tauschii-rice CCT proteins were orthologous in the phylogenetic tree. Specific expression and constitutive expression were found in the CCT gene family; nine AetCCT genes were constitutively expressed in all organisms, including AetCCT3, AetCCT4, AetCCT7 and AetCCT9; AetCCT15, AetCCT21 and AetCCT25 were specifically expressed in leaf, seed and roots of A. tauschii, respectively. Moreover, the members responded to phytohormone treatments differently, which suggested a complex function and characteristic in metabolism of this family. Light conditions affect the expression of AetCCT, and this gene family is regulated by photoperiod and vernalization. The results of this paper not only provide useful information for wheat evolution studies, but also provide theory basis for comprehensive understanding of formation and interaction characteristics of important traits.

Key words: Aegilops tauschii, CCT gene family, sequence analysis, responses to phytohormones

Zheng Jun, Qiao Ling, Zhao Jiajia, Qiao Linyi, Zhang Shichang, Chang Jianzhong, Tang Caiguo, Yang Sanwei. Whole-genome Analysis of CCT Gene Family and Their Responses to Phytohormones in Aegilops tauschii[J]. Chinese Bulletin of Botany, 2017, 52(2): 188-201.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB16054

https://www.chinbullbotany.com/EN/Y2017/V52/I2/188

Figures/Tables 10

References 34

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Gene	Length (bp)	CDS	Scaffold	Location	Mapped seq.	Chromosome
AetCCT1	2211	AEGTA18365	Scaffold4528	49937-52147	AT1D0317	1DL
AetCCT2	1836	AEGTA21580	Scaffold71509	42038-43873	AT1D0523	1DL
AetCCT3	1333	AEGTA27323	Scaffold97182	45576-47039	AT1D0969	1DL
AetCCT4	3096	AEGTA10532	Scaffold38896	10923-14018	AT2D1124	2DS
AetCCT5	2241	AEGTA13911	Scaffold30755	94901-97141	AT2D1581	2DS
AetCCT6	2157	AEGTA00203	Scaffold30755	129179-131557	AT2D1581	2DS
AetCCT7	2226	AEGTA31460	Scaffold77907	6990-9215	AT3D2607	3DS
AetCCT8	1134	AEGTA06337	Scaffold4745	59908-61041	AT3D3130	3DL
AetCCT9	2692	AEGTA28548	Scaffold28581	76532-79223	AT4D3632	4DS
AetCCT10	606	AEGTA01709	Scaffold50727	43106-43711	AT4D3683	4DS
AetCCT11	3208	AEGTA21446	Scaffold108	140572-143968	AT4D3728	4DL
AetCCT12	4521	AEGTA31746	Scaffold2864	19243-23723	AT4D3886	4DL
AetCCT13	2234	AEGTA18304	Scaffold98624	23875-26108	BE403305	4DL
AetCCT14	2878	AEGTA13770	Scaffold12030	253420-256287	AT4D4194	4DL
AetCCT15	603	AEGTA33063	Scaffold24714	5731-6333	AT5D4519	5DL
AetCCT16	1059	AEGTA03508	Scaffold185863	19051-20109	AT5D4590	5DL
AetCCT17	2404	AEGTA04421	Scaffold53469	30253-32656	AT5D5030	5DL
AetCCT18	738	AEGTA32221	Scaffold7166	12609-13346	AT5D5201	5DL
AetCCT19	3597	AEGTA05461	Scaffold106936	10967-14734	AT6D5284	6DS
AetCCT20	1188	AEGTA21198	Scaffold137524	2620-3965	AT6D5798	6DL
AetCCT21	2031	AEGTA15475	Scaffold71269	16605-18635	AT7D6397	7DS
AetCCT22	2781	AEGTA31079	Scaffold66553	48279-51059	AT7D6416	7DS
AetCCT23	3075	AEGTA08066	Scaffold6305	52570-55644	AT7D6716	7DS
AetCCT24	1521	AEGTA13638	Scaffold7100	34283-35803	AT7D7002	7DL
AetCCT25	742	AEGTA03492	Scaffold116052	16157-16929	AT7D7027	7DL
AetCCT26	2036	AEGTA22574	Scaffold16211	9228-11710	AT7D7167	7DL

Gene	Length (bp)	CDS	Scaffold	Location	Mapped seq.	Chromosome
AetCCT1	2211	AEGTA18365	Scaffold4528	49937-52147	AT1D0317	1DL
AetCCT2	1836	AEGTA21580	Scaffold71509	42038-43873	AT1D0523	1DL
AetCCT3	1333	AEGTA27323	Scaffold97182	45576-47039	AT1D0969	1DL
AetCCT4	3096	AEGTA10532	Scaffold38896	10923-14018	AT2D1124	2DS
AetCCT5	2241	AEGTA13911	Scaffold30755	94901-97141	AT2D1581	2DS
AetCCT6	2157	AEGTA00203	Scaffold30755	129179-131557	AT2D1581	2DS
AetCCT7	2226	AEGTA31460	Scaffold77907	6990-9215	AT3D2607	3DS
AetCCT8	1134	AEGTA06337	Scaffold4745	59908-61041	AT3D3130	3DL
AetCCT9	2692	AEGTA28548	Scaffold28581	76532-79223	AT4D3632	4DS
AetCCT10	606	AEGTA01709	Scaffold50727	43106-43711	AT4D3683	4DS
AetCCT11	3208	AEGTA21446	Scaffold108	140572-143968	AT4D3728	4DL
AetCCT12	4521	AEGTA31746	Scaffold2864	19243-23723	AT4D3886	4DL
AetCCT13	2234	AEGTA18304	Scaffold98624	23875-26108	BE403305	4DL
AetCCT14	2878	AEGTA13770	Scaffold12030	253420-256287	AT4D4194	4DL
AetCCT15	603	AEGTA33063	Scaffold24714	5731-6333	AT5D4519	5DL
AetCCT16	1059	AEGTA03508	Scaffold185863	19051-20109	AT5D4590	5DL
AetCCT17	2404	AEGTA04421	Scaffold53469	30253-32656	AT5D5030	5DL
AetCCT18	738	AEGTA32221	Scaffold7166	12609-13346	AT5D5201	5DL
AetCCT19	3597	AEGTA05461	Scaffold106936	10967-14734	AT6D5284	6DS
AetCCT20	1188	AEGTA21198	Scaffold137524	2620-3965	AT6D5798	6DL
AetCCT21	2031	AEGTA15475	Scaffold71269	16605-18635	AT7D6397	7DS
AetCCT22	2781	AEGTA31079	Scaffold66553	48279-51059	AT7D6416	7DS
AetCCT23	3075	AEGTA08066	Scaffold6305	52570-55644	AT7D6716	7DS
AetCCT24	1521	AEGTA13638	Scaffold7100	34283-35803	AT7D7002	7DL
AetCCT25	742	AEGTA03492	Scaffold116052	16157-16929	AT7D7027	7DL
AetCCT26	2036	AEGTA22574	Scaffold16211	9228-11710	AT7D7167	7DL