Chinese Bulletin of Botany ›› 2017, Vol. 52 ›› Issue (2): 188-201.DOI: 10.11983/CBB16054

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Whole-genome Analysis of CCT Gene Family and Their Responses to Phytohormones in Aegilops tauschii

Zheng Jun1, Qiao Ling1, Zhao Jiajia1, Qiao Linyi2, Zhang Shichang2, Chang Jianzhong2, Tang Caiguo3,*(), Yang Sanwei1,*()   

  1. 1Institute of Wheat Research, Shanxi Academy of Agricultural Sciences, Linfen 041000, China
    2Shanxi Key Laboratory of Crop Genetics and Molecular Improvement, Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China
    3Hefei Institutes of Physical Science, Institute of Technical Biology & Agriculture Engineering, Chinese Academy of Sciences, Hefei 230031, China
  • Received:2016-03-18 Accepted:2016-08-04 Online:2017-03-01 Published:2017-04-05
  • Contact: Tang Caiguo,Yang Sanwei
  • About author:# Co-first authors


The control of flowering time is a crucial environmental adaptation in plants; numerous CCT domain genes control flowering in plants. Bioinformatics was used for a genome-wide research of CCT domain genes in Aegilops tauschii. In this study, 26 CCT domain genes were identified in A. tauschii, distributed on seven chromosomes of the A. tauschii genome. The predicted molecular weight of this family was spread over 14.9 to 83.2 kDa, and 25 proteins contain a complete CCT conserved structure domain. Twelve pairs of A. tauschii-Triticum urartu and 9 pairs of A. tauschii-rice CCT proteins were orthologous in the phylogenetic tree. Specific expression and constitutive expression were found in the CCT gene family; nine AetCCT genes were constitutively expressed in all organisms, including AetCCT3, AetCCT4, AetCCT7 and AetCCT9; AetCCT15, AetCCT21 and AetCCT25 were specifically expressed in leaf, seed and roots of A. tauschii, respectively. Moreover, the members responded to phytohormone treatments differently, which suggested a complex function and characteristic in metabolism of this family. Light conditions affect the expression of AetCCT, and this gene family is regulated by photoperiod and vernalization. The results of this paper not only provide useful information for wheat evolution studies, but also provide theory basis for comprehensive understanding of formation and interaction characteristics of important traits.

Key words: Aegilops tauschii, CCT gene family, sequence analysis, responses to phytohormones