Chinese Bulletin of Botany ›› 2020, Vol. 55 ›› Issue (2): 192-198.DOI: 10.11983/CBB19223

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Optimization of Tissue Culture and Plant Regeneration System of Mature Embryo of Leymus chinensis

Yan Xiao1,Zhenxing Wang1,Dongming Li2,Yanhua Qi2, Enhebayaer1()   

  1. 1 College of Life Science and Technology, Inner Mongolia Normal University, Huhehot 010022, China
    2 Key Laboratory of Herbage and Endemic Crop Biotechnology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Huhehot 010000, China
  • Received:2019-11-17 Accepted:2020-02-26 Online:2020-03-01 Published:2020-02-26
  • Contact: Enhebayaer

Abstract: Leymus chinensis is a heterotetraploid grass. It is hard to use genetic transformation method for improving breeding since the efficiency of regeneration using callus induced from mature embryo is very low. In this study, we optimized the protocol of culturing mature embryo with L. chinensis as explants by screening optimum plant hormone concentrations, light conditions and culturing temperatures at different culturing stages including callus induction, differentiation, rooting and transplanting. Our results showed at the callus induction stage, the induction rate could reach up to 74.1% under 2.0 mg·L -12,4-D, variable temperature and dark culture. The differentiation rate could reach up to 57.1% with optimum condition of 1.0 mg·L -16-BA and 1.0 mg·L -1NAA. In the rooting stage, the survival rate was 100% when cultured with 0.25 mg·L -1NAA.

Key words: mature embryo, Leymus chinensis, callus, plant hormone, plant regeneration