Transformation of RNAi Vector in Cucumber (Cucumis sativus) In Vitro by Agrobacterium tumefaciens-mediated Transfection

doi:10.3724/SP.J.1259.2014.00183

Abstract

Abstract: The RNAi vector of the target gene was transfected into cucumber (Cucumis sativus) cotyledons and cotyledon nodes by Agrobacterium tumefaciens-mediated transfection. A total of 99 regeneration plants were hygromycin-resistant. PCR and Southern-blot analysis revealed that target gene was integrated into the genome of 10 regenerated plants. The transformation efficiency in the cucumber cotyledon and cotyledon node was significantly increased from 4.1% to 13.9% and 3.1% to 6.9%, respectively, when using different hygromycin concentrations in the selection cultivation. We have optimized the genetic transformation system with an RNA interference vector in cucumber and successfully obtained transgenic cucumber plants.

Key words: cucumber, RNAi vector, cotyledon, cotyledon node, transformation

Ye Wang, Xingfang Gu, Shengping Zhang, Han Miao, Guohua Chen, Bingyan Xie. Transformation of RNAi Vector in Cucumber (Cucumis sativus) In Vitro by Agrobacterium tumefaciens-mediated Transfection[J]. Chinese Bulletin of Botany, 2014, 49(2): 183-189.

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URL: https://www.chinbullbotany.com/EN/10.3724/SP.J.1259.2014.00183

https://www.chinbullbotany.com/EN/Y2014/V49/I2/183

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