Cloning and Expression Analysis of Calcium-dependent Phosphokinase Genes in Chenopodium album Under Stresses
Shasha Chen, Zhuanzhuan He, Shengxiu Jiang, Jiajia Xing, Xiuyun Lü, Haiyan Lan
Chinese Bulletin of Botany. 2014, 49(2):
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By homology cloning, we obtained the core sequences of 7 calcium-dependent protein kinase (CDPK) genes, CaCPK1 to CaCPK7, from salt-tolerant Chenopodium album. Quantitative real-time PCR revealed that CaCPK1, CaCPK2, CaCPK3 and CaCPK4 were significantly induced by salt stress with different patterns. The expression pattern of CaCPK1, CaCPK2 and CaCPK3 was also changed in C. album under both H2O2 and abscisic acid (ABA) stresses. Diphenylene iodonium (DPI, inhibitor of H2O2 synthesis) and sodium tungstate (Na2WO4, inhibitor of ABA synthesis) significantly inhibited the expression of CaCPK1, CaCPK2 and CaCPK3 on exposure to 300 mmol·L–1NaCl. We obtained the full-length sequences of 3 CDPKs―CaCPK1, CaCPK2 and CaCPK3―by 3′- and 5′-RACE, which contain open reading frames of 1 632, 1 704, and 1 590 bp, respectively. The production of ABA and H2O2 induced by salt may be involved in induction of CaCPK1, CaCPK2 and CaCPK3, facilitating the analysis of the function of CDPK gene family in C. album.