Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (6): 711-722.DOI: 10.11983/CBB19042

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Expression Characterization of Rice OsPR10A and Its Function in Response to Drought Stress

Tong Zhang1,Yalu Guo1,2,Yue Chen1,Jinjiao Ma1,Jinping Lan1,3,Gaowei Yan1,Yuqing Liu1,Shan Xu1,Liyun Li1,Guozhen Liu1,*(),Shijuan Dou1,*()   

  1. 1 College of Life Sciences, Hebei Agricultural University, Baoding 071001, China
    2 Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518116, China
    3 Research Center for Life Sciences, Hebei North University, Zhangjiakou 075000, China
  • Received:2019-02-27 Accepted:2019-05-06 Online:2019-11-01 Published:2020-07-09
  • Contact: Guozhen Liu,Shijuan Dou

Abstract:

In this study, we surveyed the expression patterns of rice OsPR10A protein by using western blot analysis for protein samples extracted from different tissues at different developmental stages and a variety of abiotic stresses. The expression of OsPR10A was upregulated significantly under drought, salt stress and hormone treatments such as methyl jasmonate and abscisic acid, which suggests that OsPR10A protein plays a role in drought and salt stress response. To test this hypothesis, we constructed a vector for overexpression of OsPR10A protein and obtained homozygous transgenic lines via Agrobacterium-mediated rice transformation. Phenotypic investigation in field experiments revealed shorter plant height and spike length as well as lower seed setting rate in transgenic lines than controls. At seed germination stage, drought stress treatment was applied by using 20% PEG6000. Root and shoot length was significantly longer in OsPR10A overexpression lines than the wild-type control, which supports that overexpression of OsPR10A protein enhanced the drought tolerance of rice during germination. The study increases our understanding of the function of rice OsPR10A protein.

Key words: rice, drought stress, pathogenesis-related protein, western blot, antibody-based proteomics