植物学报 ›› 2014, Vol. 49 ›› Issue (3): 246-253.DOI: 10.3724/SP.J.1259.2014.00246 cstr: 32102.14.SP.J.1259.2014.00246
李春宏, 付三雄, 戚存扣*
收稿日期:
2013-08-05
修回日期:
2013-10-20
出版日期:
2014-05-01
发布日期:
2014-06-03
通讯作者:
戚存扣
基金资助:
CRABS CLAW(CRC)在油菜花发育过程中的调控机理研究;甘蓝型油菜突变体FS-M1雌性不育机理及其相关基因克隆
Chunhong Li, Sanxiong Fu, Cunkou Qi*
Received:
2013-08-05
Revised:
2013-10-20
Online:
2014-05-01
Published:
2014-06-03
Contact:
Cunkou Qi
摘要: 以甘蓝型油菜(Brassica napus)野生型(宁油10号)及其柱头授粉功能缺失突变体FS-M1为材料, 使用油菜基因表达谱芯片筛选甘蓝型油菜柱头特异表达基因。在含有16 540个基因的油菜基因表达谱芯片中(43 803探针), 获得了4 410条差异表达探针, 选择部分差异表达基因进行实时定量PCR, 所得结果与芯片检测结果相吻合。其中, 野生型较FS-M1显著上调且获得209个功能注释的探针, 对应198个基因, 这些特异表达的基因主要富集在水解酶、转移酶、氧化还原酶和转录因子中; 涉及较大的基因家族包括: 细胞色素P450基因、GDSL脂肪酶/水解酶基因、ABC转运蛋白基因、myb转录因子基因、bHLH转录因子基因、过氧化物酶家族和受体激酶基因等。推测这些基因与甘蓝型油菜柱头发育及授粉功能有关。
李春宏, 付三雄, 戚存扣. 应用基因芯片分析甘蓝型油菜柱头特异表达基因. 植物学报, 2014, 49(3): 246-253.
Chunhong Li, Sanxiong Fu, Cunkou Qi. Microarray Analysis of Genes Expressed in Brassica napus Stigma. Chinese Bulletin of Botany, 2014, 49(3): 246-253.
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