甘蓝型油菜转录因子BnaABF2的表征分析及互作蛋白鉴定

doi:10.11983/CBB24019

摘要/Abstract

摘要： ABF转录因子是能够特异识别并结合ABA响应元件(ABRE)的碱性亮氨酸拉链蛋白的统称, 参与ABA的信号转导并作为ABA信号转录应答的调节因子。本研究对甘蓝型油菜(Brassica napus) BnaABF2基因编码的蛋白进行了分析。亚细胞定位结果显示, BnaABF2蛋白定位于细胞核中; 酵母系统转录活性分析表明, BnaABF2无转录激活活性; qRT-PCR检测发现, BnaABF2的表达量在叶中最高。我们还发现ABA处理、模拟干旱、盐逆境能够诱导BnaABF2的表达; BiFC结果显示, BnaMPK1/2/6/7/9/12/13能与BnaABF2互作。Dual-LUC结果表明, BnaMPK7可能通过磷酸化增强BnaABF2对下游靶基因的转录调控。本研究初步探索了转录因子基因BnaABF2的基本特性与互作蛋白, 对于理解其功能与机制具有一定的理论指导意义。

关键词: 甘蓝型油菜, ABF2, 亚细胞定位, MPK, 互作蛋白

Abstract: ABF transcription factors are collectively referred to as basic leucine zipper proteins that can specifically recognize and bind to ABA-responsive elements (ABREs), participating in ABA signal transduction and serving as regulators of ABA signal transcriptional responses. This study analyzed the protein encoded by the BnaABF2 gene in Brassica napus. Subcellular localization results showed that the BnaABF2 protein is localized in the nucleus. Analysis of transcriptional activity in the yeast system indicated that BnaABF2 has no transcriptional activation activity. qRT-PCR detection revealed that the expression level of BnaABF2 is highest in leaves. We also found that ABA treatment, simulated drought, and salt stress can induce the expression of BnaABF2. BiFC results showed that BnaMPK1/2/6/7/9/12/13 can interact with BnaABF2. Dual-LUC results suggested that BnaMPK7 may enhance the transcriptional regulation of BnaABF2 on downstream target genes through phosphorylation. This study initially explored the basic characteristics and interacting proteins of the transcription factor gene BnaABF2, providing theoretical guidance for understanding its functions and mechanisms.

Key words: Brassica napus, ABF2, Subcellular localization, MPK, interaction proteins

杨柳卿, 王劲, 燕敬利, 陈芹芹, 程浩坤, 李春, 赵培玉, 杨博, 江元清. 甘蓝型油菜转录因子BnaABF2的表征分析及互作蛋白鉴定. 植物学报, DOI: 10.11983/CBB24019.

Liuqing Yang, Jing Wang, Jingli Yan, Qinqin Chen, Haokun Chen, Chun Li, Peiyu Zhao, Bo Yang, Yuanqing Jiang. Analysis of Expression Characteristics and Identification of Interaction Proteins of BnaABF2 Transcription Factor in Brassica napus . Chinese Bulletin of Botany, DOI: 10.11983/CBB24019.

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