植物学报 ›› 2025, Vol. 60 ›› Issue (1): 49-61.DOI: 10.11983/CBB24019  cstr: 32102.14.CBB24019

• 研究报告 • 上一篇    下一篇

甘蓝型油菜转录因子BnaABF2的表征分析及互作蛋白鉴定

杨柳卿,, 王劲,, 燕敬利, 陈芹芹, 程浩坤, 李春, 赵培玉, 杨博, 江元清*()   

  1. 西北农林科技大学生命科学学院, 杨凌 712100
  • 收稿日期:2024-02-05 接受日期:2024-08-20 出版日期:2025-01-10 发布日期:2024-08-22
  • 通讯作者: * 江元清, 男, 汉族, 湖南省桃源县人, 教授, 博士生导师。1998年6月本科毕业于湖南农业大学资环学院; 2001年6月硕士研究生毕业于中国农业大学生物学院生物化学与分子生物学专业。2001.07-2003.08在北京市农林科学院农业生物技术研究中心工作(助理研究员), 从事扁桃基因功能分析相关研究。2003.09-2007.11在加拿大阿尔伯特大学(University of Alberta)生物科学系学习(植物生物学专业), 获博士学位。2007.12-2010.11先后在阿尔伯特大学生物科学系和美国加州大学伯克利分校(University of California-Berkeley)植物与微生物学系做博士后研究。2010年12月就职于西北农林科技大学生命科学学院, 从事教学与科研工作。E-mail: jiangyq@nwafu.edu.cn
  • 作者简介:共同第一作者。
  • 基金资助:
    国家自然科学基金(32171953)

Analysis of Expression Characteristics and Identification of Interaction Proteins of BnaABF2 Transcription Factor in Brassica napus

Liuqing Yang,, Jin Wang,, Jingli Yan, Qinqin Chen, Haokun Cheng, Chun Li, Peiyu Zhao, Bo Yang, Yuanqing Jiang*()   

  1. College of Life Sciences, Northwest A & F University, Yangling 712100, China
  • Received:2024-02-05 Accepted:2024-08-20 Online:2025-01-10 Published:2024-08-22
  • Contact: * E-mail: jiangyq@nwafu.edu.cn
  • About author:These authors contributed equally to this paper

摘要: ABF转录因子是能够特异识别并结合ABA响应元件(ABRE)的碱性亮氨酸拉链蛋白的统称, 参与ABA信号转导。通过对甘蓝型油菜(Brassica napus) BnaABF2基因编码蛋白进行分析, 亚细胞定位结果显示, BnaABF2蛋白定位于细胞核; 酵母系统转录活性分析表明, BnaABF2无转录激活活性; qRT-PCR检测发现, BnaABF2在叶中的表达量最高。此外, 还发现ABA处理、模拟干旱和盐胁迫能够诱导BnaABF2的表达; BiFC结果显示, BnaMPK1/2/6/7/9/12/13能与BnaABF2相互作用。Dual-LUC结果表明, BnaMPK7可能通过磷酸化增强BnaABF2对下游靶基因的转录调控。该研究初步探索了转录因子BnaABF2的基本特性与互作蛋白, 对理解其功能与机制具有一定的理论价值。

关键词: 甘蓝型油菜, ABF2, 亚细胞定位, MPK, 互作蛋白

Abstract: ABF transcription factors are collectively referred to as basic leucine zipper proteins that can specifically recognize and bind to ABA-responsive elements (ABRE), participating in ABA signal transduction and serving as regulators of ABA signal transcriptional responses. This study analyzed the protein encoded by the BnaABF2 gene in Brassica napus. Subcellular localization results showed that the BnaABF2 protein is localized in the nucleus. Analysis of transcriptional activity in the yeast system indicated that BnaABF2 has no transcriptional activation activity; qRT-PCR detection revealed that the expression level of BnaABF2 is highest in leaves. We also found that ABA treatment, simulated drought, and salt stress can induce the expression of BnaABF2; BiFC results showed that BnaMPK1/2/6/7/9/12/13 can interact with BnaABF2. Dual-LUC results suggested that BnaMPK7 may enhance the transcriptional regulation of BnaABF2 on downstream target genes through phosphorylation. This study initially explored the basic characteristics and interacting proteins of the transcription factor BnaABF2, providing theoretical guidance for understanding its functions and mechanisms.

Key words: Brassica napus, ABF2, subcellular localization, MPK, interaction proteins