应用基因芯片分析甘蓝型油菜柱头特异表达基因

doi:10.3724/SP.J.1259.2014.00246

植物学报 ›› 2014, Vol. 49 ›› Issue (3): 246-253.DOI: 10.3724/SP.J.1259.2014.00246

应用基因芯片分析甘蓝型油菜柱头特异表达基因

李春宏, 付三雄, 戚存扣^*

江苏省农业科学院经济作物研究所, 国家油菜改良中心南京分中心, 南京 210014

收稿日期:2013-08-05 修回日期:2013-10-20 出版日期:2014-05-01 发布日期:2014-06-03
通讯作者: 戚存扣
基金资助:
CRABS CLAW(CRC)在油菜花发育过程中的调控机理研究;甘蓝型油菜突变体FS-M1雌性不育机理及其相关基因克隆

Microarray Analysis of Genes Expressed in Brassica napus Stigma

Chunhong Li, Sanxiong Fu, Cunkou Qi^*

Nanjing Sub-center of National Rapeseed Development Center, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2013-08-05 Revised:2013-10-20 Online:2014-05-01 Published:2014-06-03
Contact: Cunkou Qi

摘要/Abstract

摘要： 以甘蓝型油菜(Brassica napus)野生型(宁油10号)及其柱头授粉功能缺失突变体FS-M1为材料, 使用油菜基因表达谱芯片筛选甘蓝型油菜柱头特异表达基因。在含有16 540个基因的油菜基因表达谱芯片中(43 803探针), 获得了4 410条差异表达探针, 选择部分差异表达基因进行实时定量PCR, 所得结果与芯片检测结果相吻合。其中, 野生型较FS-M1显著上调且获得209个功能注释的探针, 对应198个基因, 这些特异表达的基因主要富集在水解酶、转移酶、氧化还原酶和转录因子中; 涉及较大的基因家族包括: 细胞色素P450基因、GDSL脂肪酶/水解酶基因、ABC转运蛋白基因、myb转录因子基因、bHLH转录因子基因、过氧化物酶家族和受体激酶基因等。推测这些基因与甘蓝型油菜柱头发育及授粉功能有关。

Abstract: Genes expressed in stigma were screened for a pollination-deficient mutant FS-M1 in Brassica napus and its wild type (NY10) by using B. napus gene microarray. Among the 16 540 genes (43 803 probes) represented on the array, 4 410 showed significant differential expression; the differential expression of selected genes was validated by qRT-PCR. We identified 198 genes (209 probes) that were significantly upregulated in the wild-type stigma and were markedly enriched in function of hydrolase activity, transferase activity, oxidoreductase activity and transcription factors, and some considerable gene families were involved, including cytochrome P450, GDSL-motif lipase, ABC transporter, myb transcription factor, bHLH transcription factor, peroxidase and receptor kinase, which may be related to development and pollination of B. napus stigma.

李春宏, 付三雄, 戚存扣. 应用基因芯片分析甘蓝型油菜柱头特异表达基因. 植物学报, 2014, 49(3): 246-253.

Chunhong Li, Sanxiong Fu, Cunkou Qi. Microarray Analysis of Genes Expressed in Brassica napus Stigma. Chinese Bulletin of Botany, 2014, 49(3): 246-253.

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应用基因芯片分析甘蓝型油菜柱头特异表达基因

Microarray Analysis of Genes Expressed in Brassica napus Stigma

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