植物学报 ›› 2010, Vol. 45 ›› Issue (05): 548-555.DOI: 10.3969/j.issn.1674-3466.2010.05.004

• 研究报告 • 上一篇    下一篇

拟南芥与大豆疫霉菌的非寄主互作及一个感病突变体的遗传分析

刘秋萍1,4, 曹华2,4, 姚茂金3,4, 马英3,4, 邓斌生3,4, 权军利4, 单卫星1,4*   

  1. 1西北农林科技大学植物保护学院, 杨凌 712100;
    2西北农林科技大学生命科学学院, 杨凌 712100
    3西北农林科技大学农学院, 杨凌 712100;
    4西北农林科技大学陕西省农业分子生物学重点实验室, 杨凌 712100
  • 收稿日期:2009-09-23 修回日期:2009-12-23 出版日期:2010-09-01 发布日期:2010-09-20
  • 通讯作者: 单卫星

Nonhost Interaction of Phytophthora sojae and Arabidopsis thaliana and Genetic Analysis of a Susceptible Mutant

Qiuping Liu1,4, Hua Cao2,4, Maojin Yao3,4, Ying Ma3,4, Binsheng Deng3,4, Junli Quan4, Weixing Shan1,4*   

  1. 1College of Plant Protection, Northwest A & F University, Yangling 712100, China;

    2College of Life Sciences, Northwest A & F University, Yangling 712100, China;

    3College of Agronomy, Northwest A & F University, Yangling 712100, China;

    4Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, China
  • Received:2009-09-23 Revised:2009-12-23 Online:2010-09-01 Published:2010-09-20
  • Contact: Weixing Shan

摘要: 非寄主抗病性是一种普遍的自然现象, 该文通过建立拟南芥-大豆疫霉菌(Arabidopsis thaliana-Phytophthora sojae)非寄主互作系统, 筛选对大豆疫霉菌感病的拟南芥突变体, 为研究植物对卵菌的非寄主抗病性遗传机制奠定基础。以大豆疫霉菌游动孢子接种拟南芥T-DNA插入突变体离体叶片, 从代表12 000个独立转化株系的40 000株T3代T-DNA插入拟南芥突变体中获得一系列对大豆疫霉菌感病的突变体。其中突变体581-51感病性状表现稳定, 离体叶片接菌后3天内出现明显的水渍状病斑, 4–5天后产生大量卵孢子和/或孢子囊。细胞学观察发现有典型的吸器形成。Southern杂交和遗传分析结果表明, 581-51突变体含有4个T-DNA插入事件, 其感病性状可能由隐性单基因控制。

Abstract: Phytophthora sojae and Arabidopsis thaliana were used in this study as a nonhost plant-oomycete interaction system to investigate the genetic basis of nonhost resistance against oomycete pathogens in plants. A collection of more than 40 000 T3 A. thaliana T-DNA mutant plants representing 12 000 independent insertion lines were screened by inoculating detached leaves with P. sojae zoospores, and the susceptible mutant was re-confirmed by pathogen inoculation and cytological characterization. A number of P. sojae-susceptible A. thaliana mutants were successfully obtained, and one of them, mutant 581-51, was shown to be stably susceptible to P. sojae infection. Water-soaked lesions formed on the detached leaves within 3 days, as did oospores and sporangia 4–5 days after inoculation with P. sojae zoospores. Cytological characterization revealed the formation of haustoria-like structures. Southern analysis showed the presence of four T-DNA insertion events in the mutant. Genetic analysis indicated that the susceptibility to infection by the nonhost pathogen P. sojae in the mutant 581-51 was likely controlled by a single recessive gene.