褪黑素对铅胁迫下虎舌红和朱砂根生理响应及DNA损伤的调控效应

doi:10.11983/CBB21191

植物学报 ›› 2022, Vol. 57 ›› Issue (2): 171-181.DOI: 10.11983/CBB21191

褪黑素对铅胁迫下虎舌红和朱砂根生理响应及DNA损伤的调控效应

艾金祥¹, 宋嘉怡¹, 严浙楠¹, 王志超¹, 陈文倩¹, 吴玉环²^,³, 王燕燕¹, 潘蕾蕾¹, 许俞韬¹, 刘鹏¹^,^*()

¹浙江师范大学植物学实验室, 金华 321004
²杭州师范大学生命与环境科学学院, 杭州 310036
³中国科学院沈阳应用生态研究所, 沈阳 110016

收稿日期:2021-11-10 接受日期:2022-02-07 出版日期:2022-03-01 发布日期:2022-03-24
通讯作者: 刘鹏
作者简介:*E-mail: sky79@zjnu.cn
基金资助:
国家自然科学基金(41571049);国家自然科学基金(30540056);浙江省自然科学基金(Y5100390);浙江省公益技术农业项目(2015C32127)

Effects of Exogenous Melatonin on Physiological Response and DNA Damage of Ardisia mamillata and A. crenata Under Lead Stress

Jinxiang Ai¹, Jiayi Song¹, Zhenan Yan¹, Zhichao Wang¹, Wenqian Chen¹, Yuhuan Wu²^,³, Yanyan Wang¹, Leilei Pan¹, Yutao Xu¹, Peng Liu¹^,^*()

¹Botany Laboratory, Zhejiang Normal University, Jinhua 321004, China
²College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China
³Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China

Received:2021-11-10 Accepted:2022-02-07 Online:2022-03-01 Published:2022-03-24
Contact: Peng Liu

摘要/Abstract

摘要： 铅胁迫是影响植物生长的主要非生物因素之一。以二年生虎舌红(Ardisia mamillata)和朱砂根(A. crenata)为实验材料, 探究外源褪黑素(MT)处理对不同浓度铅胁迫下2种植物生理响应及DNA损伤的调控效应。结果表明, 相同处理时间内, 随着铅胁迫浓度的升高, 虎舌红与朱砂根的3种抗氧化酶活性、脯氨酸(Pro)及可溶性蛋白(SP)含量均先升高后降低, 而丙二醛(MDA)含量先降低后升高, 根尖胼胝质含量持续升高, 根系DNA损伤加剧。施加适宜浓度的外源褪黑素(MT)后, 不同浓度铅胁迫下2种植物的抗氧化酶活性得到有效增强, 且均在100 µmol∙L^-1 MT处理时达到最大值, Pro和SP含量均显著升高, MDA含量则先显著降低而后缓慢升高, 根尖胼胝质含量持续增加, 根系DNA损伤得到改善; 随着MT浓度的持续升高, 其缓解作用逐渐减弱。相较于虎舌红, 朱砂根对铅胁迫的抗性更强, 生理响应更稳定。施加外源MT可有效缓解铅胁迫对虎舌红和朱砂根的毒害作用(缓解效果朱砂根>虎舌红), 增强二者对铅毒的耐受性, 其中100 µmol∙L^-1 MT处理下缓解效果最佳。研究揭示了虎舌红和朱砂根抗铅性的优劣及外源MT对铅毒的缓解效应, 为紫金牛属植物抗铅性研究提供理论参考。

关键词: 虎舌红, 朱砂根, 铅胁迫, 褪黑素, DNA损伤

Abstract: Lead (Pb) is a major abiotic constraint affecting plant growth. To compare the anti-lead ability of two Ardisiaspecies and investigate the physiological responses and DNA damage to the treatments of exogenous melatonin (MT) under lead stress, hydroponic experiment was carried out with 2-year-old seedlings of A. mamillata and A. crenata. The results showed that with the increasing of the lead stress concentration in the same period of time, the three antioxidant enzyme activities, proline (Pro) and soluble protein (SP) contents of the A. mamillata and A. crenata were all increased first and then decreased, while malondialdehyde (MDA) content decreased at first and then increased, the root tip callosin content continues to rise, DNA damage in roots was increased. After the application of appropriate concentrations of exogenous MT, the activities of antioxidant enzymes in both plants were effectively enhanced and reached a maximum at 100 µmol∙L^-1 MT treatment under different concentrations of Pb, and the contents of Pro and SP were significantly increased. However, the MDA content decreased significantly at first and then increased slowly. The root tip callosin content continues to increase and the root DNA damage was improved. With the increasing of MT concentration, the alleviating effect of melatonin on A. mamillata and A. crenata gradually weakened. A. crenata is more resistant to lead stress and its physiological response is more stable than A. mamillata. Exogenous application of MT can effectively alleviate the toxic effect of lead stress on A. mamillata and A. crenata (relief effect of A. crenata > A. mamillata), and enhance its tolerance to lead toxicity, among which 100 µmol∙L^-1 MT treatment has the best mitigation effect. We revealed the superiority of lead resistance of A. mamillata and A. crenata, and the alleviating effect of exogenous MT on lead poisoning, thus providing a theoretical reference for the study of lead resistance of Ardisia.

Key words: Ardisia mamillata, A. crenata, lead stress, melatonin, DNA damage

艾金祥, 宋嘉怡, 严浙楠, 王志超, 陈文倩, 吴玉环, 王燕燕, 潘蕾蕾, 许俞韬, 刘鹏. 褪黑素对铅胁迫下虎舌红和朱砂根生理响应及DNA损伤的调控效应. 植物学报, 2022, 57(2): 171-181.

Jinxiang Ai, Jiayi Song, Zhenan Yan, Zhichao Wang, Wenqian Chen, Yuhuan Wu, Yanyan Wang, Leilei Pan, Yutao Xu, Peng Liu. Effects of Exogenous Melatonin on Physiological Response and DNA Damage of Ardisia mamillata and A. crenata Under Lead Stress. Chinese Bulletin of Botany, 2022, 57(2): 171-181.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB21191

https://www.chinbullbotany.com/CN/Y2022/V57/I2/171

图/表 6

图1 不同浓度褪黑素处理对铅胁迫下虎舌红(A-C)和朱砂根(D-F)抗氧化酶系统的影响 Pb0、Pb100和Pb600表示铅浓度分别为0、100和600 µmol∙L-1; MT0、MT50、MT100、MT150和MT200表示褪黑素处理浓度分别为0、50、100、150和200 µmol∙L-1。不同小写字母表示相同时期不同处理组间在0.05水平差异显著。

Figure 1 Effects of different concentrations of melatonin on antioxidant enzyme system of Ardisia mamillata (A-C) and A. crenata (D-F) under lead stress Pb0, Pb100, and Pb600 represent 0, 100, and 600 µmol∙L-1 of Pb, respectively; MT0, MT50, MT100, MT150, and MT200 represent 0, 50, 100, 150, and 200 µmol∙L-1 of melatonin, respectively. Different lowercase letters indicate significant differences among the treatments during the same stage at 0.05 level.

图2 不同浓度褪黑素对铅胁迫下虎舌红与朱砂根叶片丙二醛(MDA) (A, B)、脯氨酸(Pro) (C, D)和可溶性蛋白(SP) (E, F)含量的影响 (A), (C), (E) 虎舌红; (B), (D), (F) 朱砂根。Pb0、Pb100、Pb600、MT0、MT50、MT100、MT150和MT200同图1。不同小写字母表示相同时期不同处理组间在0.05水平差异显著。

Figure 2 Effects of different concentrations of melatonin on the contents of malondialdehyde (MDA) (A, B), proline (Pro) (C, D) and soluble protein (SP) (E, F) in leaves of Ardisia mamillata and A. crenata under lead stress (A), (C), (E) Ardisia mamillata; (B), (D), (F) A. crenata. Pb0, Pb100, Pb600, MT0, MT50, MT100, MT150, and MT200 are the same as shown in Figure 1. Different lowercase letters indicate significant differences among the treatments during the same stage at 0.05 level.

图3 不同浓度褪黑素对铅胁迫下虎舌红与朱砂根根尖胼胝质含量的影响 Pb0、Pb100、Pb600、MT0、MT50、MT100、MT150和MT200同图1。不同小写字母表示相同时期不同处理组间在0.05水平差异显著。

Figure 3 Effects of different concentrations of melatonin on callose content in root tips of Ardisia mamillata and A. crenata under lead stress Pb0, Pb100, Pb600, MT0, MT50, MT100, MT150, and MT200 are the same as shown in Figure 1. Different lowercase letters indicate significant differences among the treatments during the same stage at 0.05 level.

图4 不同浓度褪黑素对铅胁迫下虎舌红和朱砂根彗星尾长(A)、尾部DNA含量(B)及尾距(C)的影响 Pb0、Pb100、Pb600、MT0、MT50、MT100、MT150和MT200同图1。不同小写字母表示相同时期不同处理组间在0.05水平差异显著。

Figure 4 Effect of different concentrations of melatonin on tail length (A), tail DNA content (B), and tail moment (C) of Ardisia mamillata and A. crenata under lead stress Pb0, Pb100, Pb600, MT0, MT50, MT100, MT150, and MT200 are the same as shown in Figure 1. Different lowercase letters indicate significant differences among the treatments during the same stage at 0.05 level.

图5 不同浓度褪黑素对铅胁迫下虎舌红(A)和朱砂根(B)根系DNA损伤的影响 Bars=20 μm

Figure 5 Effects of different concentrations of melatonin on DNA damage in roots of Ardisia mamillata (A) and A. crenata (B) under lead stress Bars=20 μm

图6 不同浓度褪黑素对铅胁迫下虎舌红(A)和朱砂根(B)根系Olive尾距(OTM)值的影响 Pb0、Pb100、Pb600、MT0、MT50、MT100、MT150和MT200同图1。不同小写字母表示相同时期不同处理组间在0.05水平差异显著。

Figure 6 Effects of different concentrations of melatonin on Olive tail moment (OTM) value in roots of Ardisia mamillata (A) and A. crenata (B) under lead stress Pb0, Pb100, Pb600, MT0, MT50, MT100, MT150, and MT200 are the same as in Figure 1. Different lowercase letters indicate significant differences among the treatments during the same stage at 0.05 level.

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褪黑素对铅胁迫下虎舌红和朱砂根生理响应及DNA损伤的调控效应

Effects of Exogenous Melatonin on Physiological Response and DNA Damage of Ardisia mamillata and A. crenata Under Lead Stress

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