Chin Bull Bot ›› 2019, Vol. 54 ›› Issue (6): 711-722.doi: 10.11983/CBB19042

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Expression Characterization of Rice OsPR10A and Its Function in Response to Drought Stress

Zhang Tong1,Guo Yalu1,2,Chen Yue1,Ma Jinjiao1,Lan Jinping1,3,Yan Gaowei1,Liu Yuqing1,Xu Shan1,Li Liyun1,Liu Guozhen1,*(),Dou Shijuan1,*()   

  1. 1 College of Life Sciences, Hebei Agricultural University, Baoding 071001, China
    2 Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518116, China
    3 Research Center for Life Sciences, Hebei North University, Zhangjiakou 075000, China
  • Received:2019-02-27 Accepted:2019-05-06 Online:2020-07-09 Published:2019-11-01
  • Contact: Liu Guozhen,Dou Shijuan E-mail:gzhliu@hebau.edu.cn;dsj75@126.com

Abstract:

In this study, we surveyed the expression patterns of rice OsPR10A protein by using western blot analysis for protein samples extracted from different tissues at different developmental stages and a variety of abiotic stresses. The expression of OsPR10A was upregulated significantly under drought, salt stress and hormone treatments such as methyl jasmonate and abscisic acid, which suggests that OsPR10A protein plays a role in drought and salt stress response. To test this hypothesis, we constructed a vector for overexpression of OsPR10A protein and obtained homozygous transgenic lines via Agrobacterium-mediated rice transformation. Phenotypic investigation in field experiments revealed shorter plant height and spike length as well as lower seed setting rate in transgenic lines than controls. At seed germination stage, drought stress treatment was applied by using 20% PEG6000. Root and shoot length was significantly longer in OsPR10A overexpression lines than the wild-type control, which supports that overexpression of OsPR10A protein enhanced the drought tolerance of rice during germination. The study increases our understanding of the function of rice OsPR10A protein.

Key words: rice, drought stress, pathogenesis-related protein, western blot, antibody-based proteomics

Figure 1

Expression of OsPR10A during rice growth and development (A) Expression of OsPR10A in different growth stages and tissue parts of rice (Sd: Seedling stage; Ti: Tillering stage; Bt: Booting stage; Fw: Flowering stage); (B) Expression of OsPR10A in seeds during germination; (C) Expression of OsPR10A in tissues during flowering stage (Sp: Spike; Ss: Spike-stalk; An: Anther; Husk: Husk; Low: Lower part of leaf; Mid: Middle part of leaf; Up: Upper part of leaf); (D) Expression of OsPR10A in seedling and flag leaf"

Table 1

Transcriptome analysis of the OsPR10A gene"

Library description FPKM
Shoots 50.1661
Leaves-20 day 144.7290
Pre-emergence inflorescence 4.1989
Post-emergence inflorescence 88.9886
Anther 0.5100
Pistil 2.3483
Seed-5 DAP 77.4876
Seed-10 DAP 0.0000
Embryo-25 DAP 1.5052
Endosperm-25 DAP 2.3371

Figure 2

Dynamic expression of rice OsPR10A under abiotic stress (A) Expression of OsPR10A under drought stress; (B) Expression of OsPR10A under salt stress; (C) Expression of OsPR10A modulated by exogenous MeJA; (D) Expression of OsPR10A modulated by exogenous ABA. MeJA: Methyl jasmonate; ABA: Abscisic acid"

Figure 3

Identification of OsPR10A overexpression transgenic rice A721, A726, A728 and A730 represent different transgenic lines, respectively; Lane 1-7 or 1-8 represent different plants of the same transgenic line, respectively; WT: Wildtype"

Figure 4

Phenotype of OsPR10A overexpression transgenic rice (A) Mature plant (Bar=10 cm); (B) Spike (Bar=4 cm); (C)-(F) Represent plant height, spike length, seed setting rate and tiller number, respectively. WT: Wildtype. * P<0.05; ** P<0.01"

Figure 5

Overexpression of OsPR10A enhanced the drought tolerance at rice seed germination stage (A) The germinated rice seeds at 7 days treated with 20% PEG6000 (Bar=1 cm); (B) Root length; (C) Shoot length. WT: Wildtype. * P<0.05; ** P<0.01"

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