Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (6): 773-778.DOI: 10.11983/CBB18256

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Plant Regeneration and Rapid Propagation System of Lilium bakerianum var. aureum

Wenting Zhang1,Yanhong He1,Ning Shu1,Jingjing Xing1,Baojun Liu1,Manzhu Bao1,Guofeng Liu2,*()   

  1. 1 Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
    2 Guangzhou Institute of Forestry and Landscape Architecture, Guangzhou 510405, China
  • Received:2018-11-29 Accepted:2019-03-05 Online:2019-11-01 Published:2020-07-09
  • Contact: Guofeng Liu

Abstract:

We investigated the effects of different explants, kinds and concentrations of plant growth regulators on callus induction and plant regeneration from scales of Lilium bakerianum var. aureum at tissue culture. The medium MS+1.0 mg∙L -16-BA+0.1 mg∙L -1NAA+30 g∙L -1 sucrose was suitable for inducing adventitious buds and calli. The medium MS+0.5 mg∙L -16-BA+0.1 mg∙L -1NAA+30 g∙L -1 sucrose was suitable for adventitious bud multiplication. MS+1.0 mg∙L -1NAA+30 g∙L -1 sucrose was optimal for bulblet formation from adventitious buds, and the optimal medium for the growth of bulblets was 1/2MS+0.01 mg∙L -1NAA+60 g∙L -1 sucrose. We provide technical support for germplasm resource protection and rapid propagation of L. bakerianum var. aureum.

Key words: Lilium bakerianum var. aureum, tissue culture, plant regeneration, rapid propagation, calli