Chinese Bulletin of Botany ›› 2020, Vol. 55 ›› Issue (2): 177-181.DOI: 10.11983/CBB19238

• INVITED PROTOCOL • Previous Articles     Next Articles

A Non-invasive Method for Measuring and Analyzing Circadian Phenotype in Living Plants

Yingjun Yu1,2,Hang Xu1,2,Lei Wang1,2,*()   

  1. 1 Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    2 University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2019-12-13 Accepted:2020-01-23 Online:2020-03-01 Published:2020-02-12
  • Contact: Lei Wang

Abstract: The plant circadian clock is a time-keeping molecular system, with a cycle of ~ 24 h. It was evolved to adapt to the diel rhythmic environmental cues generated by the self-rotation of the Earth. In addition to the time-keeping function, the circadian clock also regulates a plethora of plant growth and development processes by synchronizing the endogenous energy and metabolomic status. By sensing and integrating the dynamics of external environmental cues, circadian clock can coordinate gene expression at multiple levels, thus to increase the fitness of plants. Recently, there is an increasingly demand for measuring and assessing circadian phenotype for many non-circadian research field. Here, we summarized the currently available methods for detecting circadian rhythm, and show one most commonly used standard procedure for evaluating circadian phenotype in plants, which may help to provide the applicable technical assistance for the study of the circadian clock.

Key words: circadian clock, phenotype analysis, FFT-NLLS, BRASS