Analysis on the Evolution and Transcription Activation Activity of ABI4 in Brassicaceae

doi:10.11983/CBB21036

Abstract

Abstract: ABI4 is an important component of the ABA signal transduction pathway. It not only acts as a key player in ABA and GA antagonism, but also plays important roles in various aspects of ABA crosstalk with other signal chemicals. Genes regulated by ABI4 are involved in diverse processes. In this study, we got 27 homologs from 19 species in the family Brassicaceae using AthABI4 as a query, and explored their evolutionary history based on sequence polymorphism analysis, phylogenetic reconstruction, genomic synteny assay, and transcription activation activity comparison. The result revealed that ABI4 are highly conserved and might be not work as transcriptional activators independently in the Brassicaceae, which implies that the irreplaceable roles of ABI4 in plants and further research was needed to explore the molecular mechanism of its biological function.

Key words: ABI4, Brassicaceae, sequence evolution, transcriptional activation activity

Wenjing Zhang, Xiaomeng Yang, Kan Gao, Xinyi Wei, Xuetong Shi, Ruixuan Wang, Fengxia Wu, Juqing Kang. Analysis on the Evolution and Transcription Activation Activity of ABI4 in Brassicaceae[J]. Chinese Bulletin of Botany, 2021, 56(6): 676-686.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB21036

https://www.chinbullbotany.com/EN/Y2021/V56/I6/676

Figures/Tables 3

Table 1 Primers for 7 ABI4 genes in the Brassicaceae

Primer name	Primer sequence (5'-3')
AthABI4F	ACGCGTCGACATGGACCCTTTAGCTTCCCAACATCAAC
AthABI4R	ATAAGAATGCGGCCGCTTAATAGAATTCCCCCAAGATGGGATCA
AlyABI4F	ACGCGTCGACATGGACCCTTTAGCTTCCCAACAACACC
AlyABI4R	ATAAGAATGCGGCCGCTCAATAGAATTCCCTTCCTCCTTGTTCCTG
CruABI4F	ACGCGTCGACATGGACCCTTTAGCTTCCAAACAACAACAACA
CruABI4R	ATAAGAATGCGGCCGCTCAATAGAATTCTCCCAAGATAGGATCAATGA
BstABI4F	ACGCGTCGACATGGACCCTTTAGCTTCCAAACAACACC
BstABI4R	ATAAGAATGCGGCCGCTCAGTAGAATTCCCCCAAGATAGGATCA
BraABI4#1F	ACGCGTCGACATGGACCCTTTACCTTCCCAACAACAAC
BraABI4#1R	ATAAGAATGCGGCCGCTCAAAAGTCAAGCAAGAAGGGATCAACA
BraABI4#2F	ACGCGTCGACATGGACCCTTTACCTTCCCAACAAGAAC
BraABI4#2R	ATAAGAATGCGGCCGCTCAAATGTCAAGCAAGGGATCAATAAAA
EsaABI4F	ACGCGTCGACATGGACCCTTTACCTTCCCAAAAACACC
EsaABI4R	ATAAGAATGCGGCCGCTCAAAAGTCAAGCAAGAAGGGATCAACA

Figure 1 The identity and similarity of the amino acid sequences of ABI4 homologs in the Brassicaceae The upper triangle shows the identity of amino acid sequences and the lower triangle shows the similarity of amino acid sequences. Aar: Aethionema arabicum; Aly: Arabidopsis lyrata; Ath: Ar. thaliana; Bst: Boechera stricta; Bca: Brassica carinata; Bna: Br. napus; Bol: Br. oleracea var. oleracea; Bra: Br. rapa; Csa: Camelina sativa; Cgr: Capsella grandiflora; Cru: Cap. rubella; Chi: Cardamine hirsuta; Epa: Eutrema parvulum; Esa: E. salsugineum; Mer: Microthlaspi erraticum; Rra: Raphanus raphanistrum; Rsa: R. sativus; Sal: Sinapis alba; Sir: Sisymbrium irio; Tar: Thlaspi arvense

Figure 2 Phylogenetic relationships of ABI4's homologs in 20 Brassicaceae species and transcriptional activation activity of ABI4 in 6 species (A) Phylogenetic relationships (the number above the nodes indicate value in 100 represents of maximum likdihood analysis.), protein motif structure prediction and collinearity analysis of genomic mapping of ABI4's homologs in 20 Brassicaceae species; (B) The protein structure, the qualitative and quantitative detection of transcriptional activation activity of ABI4 in 6 species. Species abbreviations shown in Figure 1; Cpa: Carica papaya

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