Experimental Teaching Design for Gene Editing in Rice Breeding by CRISPR/Cas9 Technology

doi:10.11983/CBB25080

Abstract

Abstract: CRISPR/Cas9 technology, as an efficient genome editing tool, has gained increasingly widespread application in the life sciences. Incorporating this technology into university experimental teaching enables students to deepen their understanding of cutting-edge research advances while enhancing their innovative research capabilities and practical skills. In this paper, we have designed an innovative teaching experiment, “Using CRISPR/Cas9 technology to study the effect of knocking out rice SSA1 (seedling stage albino1) gene on phenotype”. This experiment helps students to deepen their understanding of the principles and experimental procedures of CRISPR/Cas9 technology and to recognize the important role played by the SSA1 gene in rice growth and development. The experiment can also improve students’ ability of experimental operation and rigorous scientific research thinking, and cultivate students’ exploratory skills and innovative spirit.

Key words: CRISPR/Cas9, gene editing, rice, experimental instructional design

Lü Ruotong, Kou Yunxia, Xiao Yujia, Ai Pengfei, Wang Yanwei. Experimental Teaching Design for Gene Editing in Rice Breeding by CRISPR/Cas9 Technology[J]. Chinese Bulletin of Botany, 2026, 61(5): 925-935.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB25080

https://www.chinbullbotany.com/EN/Y2026/V61/I5/925

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