Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (5): 701-711.DOI: 10.11983/CBB22223

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Bna-miR43 Mediates the Response of Drought Tolerance in Brassica napus

Zhang Yingchuan1, Wu Xiaomingyu1, Tao Baolong1, Chen Li1,2, Lu Haiqin1, Zhao Lun1, Wen Jing1, Yi Bin1, Tu Jinxing1, Fu Tingdong1, Shen Jinxiong1,*()   

  1. 1. National Engineering Research Center of Rapeseed/National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
    2. School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing 408100, China
  • Received:2022-09-16 Accepted:2023-02-25 Online:2023-09-01 Published:2023-09-21
  • Contact: *E-mail:

Abstract: miRNAs are involved in plant growth and response to stress by regulating the expression of target genes. We conducted a functional study on a single miRNA identified by our group, Bna-miR43. The function of Bna-miR43 under drought stress was investigated by constructing a Bna-miR43 overexpression vector. Degradation group sequencing has predicted that the four target genes of Bna-miR43 belonged to the F-box protein family. Under simulated drought conditions, the expression of Bna-miR43 in J572 roots decreased gradually, while the expression pattern of target genes was shown to be opposite to that of Bna-miR43. With the increaseing time of drought treatment, the expression of target genes increased gradually. Expression pattern analysis showed that Bna-miR43 could respond to drought stress by negatively regulating the expression of target genes. Transgenic experiment showed that under drought stress, the Bna-miR43 overexpressed lines were extremely sensitive to drought. It was shown that the germination rate of the overexpressed lines decreased significantly, the plant dehydration was serious, and more MDA and H2O2 were accumulated in the body. After drought treatment, three coding superoxide dismutase (SOD), catalase (CAT) and glycolate oxidase (GOX) were identified in the Bna-miR43 overexpressed lines, and their expressions were down-regulated in the Bna-miR43 overexpressed lines. The results showed that Bna-miR43 plays a key role in regulating plant drought tolerance by regulating the osmotic accumulation and ROS homeostasis in Brassica napus.

Key words: Brassica napus, Bna-miR43, F-box, drought stress