QTL Exploration of Bacterial Leaf Streak and Their Gene Expression in Rice

doi:10.11983/CBB17250

Abstract

Abstract: Bacterial leaf streak (BLS) is a common disease in rice and a serious threat to the high and stable production of China’s grain. We used a pair of typical indica TN1 and japonica CJ06 double haploid (DH) rice and inoculated them with Xoc BLS256, then measured and quantified the length of diseased plaques of parents and next generations. We used the molecular linkage map of this population for quantitative trait loci (QTL) mapping analysis. We found 4 QTLs that were able to distinguish the effect values located on chromosomes 2, 4, 5 and 8. We analyzed the expression of genes resistant to BLS in two large QTL intervals on chromosomes 2 and 5 and found that these genes had different degrees of expression before and after treatment. These genes might be the target genes responding to the difference in resistance to BLS of TN1 and CJ06. The results of this study lay a solid foundation for further cloning QTL for resistance to rice BLS.

Key words: bacterial leaf streak, QTL analysis, resistance, rice

Ma Lu, Fang Yuan, Xiao Saqing, Zhou Chun, Jin Zhelun, Ye Wenlan, Rao Yuchun. QTL Exploration of Bacterial Leaf Streak and Their Gene Expression in Rice[J]. Chinese Bulletin of Botany, 2018, 53(4): 468-476.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB17250

https://www.chinbullbotany.com/EN/Y2018/V53/I4/468

Figures/Tables 8

References 36

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Marker	Forward primer (5′-3′)	Reverse primer (5′-3′)
OsActin1	TGGCATCTCTCAGCACATTCC	TGCACAATGGATGGGTCAGA
LOC_Os02g10580	GGAGAGAGGCATCGTCAGTC	GCCGGAGGAGTAACCAAACA
LOC_Os02g11760	GACGGTCTTCCTGCGTACAA	CGAGCTCCGCAAATCCATTG
LOC_Os02g16250	GTACGGACAGAAGCCCATCAA	CACCCCTTCGAGCTACTGC
LOC_Os02g16540	GTACGGACAGAAGCCCATCAA	CACCCCTTCGAGCTACTGC
LOC_Os02g17190	CTGGAGCAACATCTGGCGAA	TATGGGCAACCTGAAGGAGG
LOC_Os02g18000	CGGGACATTGGAACCCTTCA	CAATGCGTCGTGTGATGGTC
LOC_Os02g18510	CCCTATGCTTCGACGGGTTT	GAGACCCACCAGTTTGTCGT
LOC_Os05g44400	TGTGCGAGCCGTATGAACAA	AAGGTCTCCTCGCCCATGTA

Marker	Forward primer (5′-3′)	Reverse primer (5′-3′)
OsActin1	TGGCATCTCTCAGCACATTCC	TGCACAATGGATGGGTCAGA
LOC_Os02g10580	GGAGAGAGGCATCGTCAGTC	GCCGGAGGAGTAACCAAACA
LOC_Os02g11760	GACGGTCTTCCTGCGTACAA	CGAGCTCCGCAAATCCATTG
LOC_Os02g16250	GTACGGACAGAAGCCCATCAA	CACCCCTTCGAGCTACTGC
LOC_Os02g16540	GTACGGACAGAAGCCCATCAA	CACCCCTTCGAGCTACTGC
LOC_Os02g17190	CTGGAGCAACATCTGGCGAA	TATGGGCAACCTGAAGGAGG
LOC_Os02g18000	CGGGACATTGGAACCCTTCA	CAATGCGTCGTGTGATGGTC
LOC_Os02g18510	CCCTATGCTTCGACGGGTTT	GAGACCCACCAGTTTGTCGT
LOC_Os05g44400	TGTGCGAGCCGTATGAACAA	AAGGTCTCCTCGCCCATGTA

Chromosome No.	QTL position (cM)	Marker interval	LOD score	Variation explained (%)	Additive effect	Dominant
2	41.5	RM145-RM521	3.1272	19.2549	-0.1729	2.6315
4	80	RM252-RM241	2.3362	10.1937	-0.3362	1.3523
5	106.6	RM6972-RM3321	2.4984	18.0963	0.18	-1.5646
8	11.5	RM6925-RM1235	2.5792	14.6327	-0.4059	-2.0904

Chromosome No.	QTL position (cM)	Marker interval	LOD score	Variation explained (%)	Additive effect	Dominant
2	41.5	RM145-RM521	3.1272	19.2549	-0.1729	2.6315
4	80	RM252-RM241	2.3362	10.1937	-0.3362	1.3523
5	106.6	RM6972-RM3321	2.4984	18.0963	0.18	-1.5646
8	11.5	RM6925-RM1235	2.5792	14.6327	-0.4059	-2.0904

Gene ID	Putative function	Gene ID	Putative function
LOC_Os02g10370	hrpN-interacting protein from Malus	LOC_Os02g17150	OsSub18-putative subtilisin homologue
LOC_Os02g11760	Pleiotropic drug resistance protein	LOC_Os02g17190	MYB family transcription factor
LOC_Os02g13890	FYVE zinc finger domain containing protein	LOC_Os02g17990	Leucine rich repeat family protein
LOC_Os02g15160	OsFBX40-F-box domain containing protein	LOC_Os02g18000	Disease resistance protein RGA2
LOC_Os02g15280	VQ domain containing protein	LOC_Os02g18140	NBS type disease resistance protein
LOC_Os02g15594	Protein phosphatase 2C	LOC_Os02g18410	Salt stress root protein RS1
LOC_Os02g15870	Molybdenu cofactor biosynthesis protein 1	LOC_Os02g18510	Stripe rust resistance protein Yr10
LOC_Os02g16070	Lr1 disease resistance protein	LOC_Os05g43820	Ras-related protein
LOC_Os02g16540	WRKY transcription factor	LOC_Os05g44550	Zinc finger family protein
LOC_Os02g16680	BZIP transcription factor	LOC_Os05g44770	Receptor-like protein kinase 5 precursor